Abstract
As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restrained by increased H2 O2 concentration stress. Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels. A total of 3,425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteins. H2 O2 -responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism. It is clear that up-regulated expression of signal transduction and stress/defence/detoxification-related proteins under H2 O2 stress, such as plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutase, could contribute to H2 O2 tolerance of wheat seedlings. Increased gluconeogenesis (phosphoenol-pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H2 O2 tolerance of wheat seedlings. A metabolic pathway of wheat seedling growth under H2 O2 stress is presented.
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Claims 7
Claim · model-written summary
Increasing hydrogen peroxide significantly restrained wheat seedling and root growth.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- increased H2 O2 concentration stresse0
- Outcome
- growth of seedlings and rootse0
- Direction
- restrainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of seedlings and roots was significantly restrained by increased H2 O2 concentration stress.”
e1“proteomic analysis of wheat seedling growth”
Claim · model-written summary
Malondialdehyde, soluble sugar and proline contents increased with increasing hydrogen peroxide.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- increasing H2 O2 levelse0
- Outcome
- Malondialdehyde, soluble sugar, and proline contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels.”
t0“wheat seedling growth”
Claim · model-written summary
Peroxidase activity increased with increasing hydrogen peroxide.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- increasing H2 O2 levelse0
- Outcome
- peroxidase activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels.”
t0“wheat seedling growth”
Claim · model-written summary
The proteomic analysis identified 3,425 proteins, including 157 differentially expressed proteins and 44 newly identified hydrogen peroxide responsive proteins.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- proteins were identified by iTRAQe0
- Value
- 3,425 proteinse0
- Qualifier
- 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 3,425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteins.”
t0“wheat seedling growth”
Claim · model-written summary
Hydrogen peroxide responsive proteins were mainly associated with stress response, signal transduction and carbohydrate metabolism.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- stress/defense/detoxification, signal transduction, and carbohydrate metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2 O2 -responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism.”
t0“wheat seedling growth”
Claim · model-written summary
Upregulated signal transduction and defense proteins could contribute to hydrogen peroxide tolerance.
- Taxon
- wheat seedlingsTriticum aestivume0
- Gene
- plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutasee0
- Outcome
- H2 O2 tolerance of wheat seedlingse0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulated expression of signal transduction and stress/defence/detoxification-related proteins under H2 O2 stress, such as plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutase, could contribute to H2 O2 tolerance of wheat seedlings.”
Claim · model-written summary
Increased gluconeogenesis and decreased pyruvate kinase proteins were potentially related to higher hydrogen peroxide tolerance.
- Taxon
- wheat seedlingsTriticum aestivume0
- Gene
- phosphoenol-pyruvate carboxykinasee0
- Outcome
- H2 O2 tolerance of wheat seedlingse0
- Direction
- highere0
- Qualifier
- potentiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased gluconeogenesis (phosphoenol-pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H2 O2 tolerance of wheat seedlings.”
The same questions in other papers
hydrogen peroxide · growth · Triticum aestivum (wheat)Results differ
4 papers in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.