Abstract
Extracellular components released from mycelia of the alpha and beta races of the bean pathogen, Colletotrichum lindemuthianum, inhibited proton uptake in sealed vesicles prepared from bean hypocotyls. Differential sensitivity of ATP-driven proton transport to nitrate, vanadate, N,N'-dicyclohexylcarbodiimide, diethylstilbestrol, and oligomycin suggested the vesicles were enriched for tonoplast. Anion stimulation of proton transport, by enhancement of ATPase activity and dissipation of the membrane potential, was consistent with this conclusion. Although fungal components inhibited the formation of a pH gradient, the membrane potential was unaffected and the ATPase activity slightly stimulated. These data suggest that the fungal components produce an electroneutral proton exchange. Proton transport in Dark Red Kidney bean tonoplast vesicles was inhibited by mycelial preparations from the incompatible alpha race and compatible beta race. Elicitor activity, however, was greater in the alpha race fractions. Elicitor purified from alpha race culture filtrate did not inhibit proton transport in vesicles isolated from Dark Red Kidney bean. Consequently, elicitor activity need not be associated with an ability to impair tonoplast function.
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Claims 11
Claim · model-written summary
Extracellular components from mycelia of the alpha and beta races of Colletotrichum lindemuthianum inhibited proton uptake in sealed vesicles from bean hypocotyls.
- Taxon
- beane0
- Organ / stage
- hypocotylse0
- Treatment
- Extracellular components released from mycelia of the alpha and beta racese0
- Interactor
- Colletotrichum lindemuthianum
Colletotrichum lindemuthianume0pathogen - Outcome
- proton uptakee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extracellular components released from mycelia of the alpha and beta races of the bean pathogen, Colletotrichum lindemuthianum, inhibited proton uptake in sealed vesicles prepared from bean hypocotyls”
Claim · model-written summary
Differential sensitivity of ATP-driven proton transport to nitrate, vanadate, dicyclohexylcarbodiimide, diethylstilbestrol and oligomycin suggested the bean hypocotyl vesicles were enriched for tonoplast.
Verbatim
e0“Differential sensitivity of ATP-driven proton transport to nitrate, vanadate, N,N'-dicyclohexylcarbodiimide, diethylstilbestrol, and oligomycin suggested the vesicles were enriched for tonoplast”
t0“Bean Hypocotyl”
Claim · model-written summary
Anion stimulation of proton transport, by enhancement of ATPase activity and dissipation of the membrane potential, was consistent with the vesicles being enriched for tonoplast.
Verbatim
e0“Anion stimulation of proton transport, by enhancement of ATPase activity and dissipation of the membrane potential, was consistent with this conclusion”
t0“Bean Hypocotyl”
Claim · model-written summary
Fungal components inhibited the formation of a pH gradient in the bean vesicles.
Not reportedComparator
Verbatim
e0“fungal components inhibited the formation of a pH gradient”
t0“Bean Hypocotyl”
Claim · model-written summary
The membrane potential of the bean vesicles was unaffected by the fungal components.
Not reportedTreatmentComparator
Verbatim
e0“the membrane potential was unaffected”
t0“Bean Hypocotyl”
Claim · model-written summary
The ATPase activity of the bean vesicles was slightly stimulated by the fungal components.
Not reportedTreatmentComparator
Verbatim
e0“the ATPase activity slightly stimulated”
t0“Bean Hypocotyl”
Claim · model-written summary
The authors suggest that the fungal components produce an electroneutral proton exchange.
Verbatim
e0“These data suggest that the fungal components produce an electroneutral proton exchange”
t0“Bean Hypocotyl”
Claim · model-written summary
Proton transport in Dark Red Kidney bean tonoplast vesicles was inhibited by mycelial preparations from the incompatible alpha race and the compatible beta race.
- Taxon
- beane0
- Material
- Dark Red Kidney beane0
- Organ / stage
- tonoplast vesiclese0
- Treatment
- mycelial preparations from the incompatible alpha race and compatible beta racee0
- Outcome
- Proton transporte0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Proton transport in Dark Red Kidney bean tonoplast vesicles was inhibited by mycelial preparations from the incompatible alpha race and compatible beta race”
Claim · model-written summary
Elicitor activity was greater in the alpha race fractions than in the beta race fractions.
Verbatim
e0“Elicitor activity, however, was greater in the alpha race fractions”
t0“Bean Hypocotyl”
Claim · model-written summary
Elicitor purified from alpha race culture filtrate did not inhibit proton transport in vesicles isolated from Dark Red Kidney bean.
- Taxon
- beane0
- Material
- Dark Red Kidney beane0
- Treatment
- Elicitor purified from alpha race culture filtratee0
- Outcome
- proton transporte0
- Direction
- did not inhibite0
Not reportedComparator
Verbatim
e0“Elicitor purified from alpha race culture filtrate did not inhibit proton transport in vesicles isolated from Dark Red Kidney bean”
Claim · model-written summary
The authors conclude that elicitor activity need not be associated with an ability to impair tonoplast function.
Verbatim
e0“elicitor activity need not be associated with an ability to impair tonoplast function”
t0“Bean Hypocotyl”
The same questions in other papers
resistance inducer · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →pathogen product · molecular: metabolites · Phaseolus vulgaris (common bean)Results agree
2 papers in the same direction as this paper
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.