Abstract
Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase complexes, which we have designated SCF(COI1). COI1(E22A), a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the SCF(COI1) complexes and results in loss of the JA response. AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression. Furthermore, we show that the AtCUL1 component of SCF(COI1) complexes is modified in planta, where mutations in AXR1 decrease the abundance of the modified AtCUL1 of SCF(COI1) and lead to a reduction in JA response. Finally, we demonstrate that the axr1 and coi1 mutations display a synergistic genetic interaction in the double mutant. These results suggest that the COI1-mediated JA response is dependent on the SCF(COI1) complexes in Arabidopsis and that the AXR1-dependent modification of the AtCUL1 subunit of SCF(COI1) complexes is important for JA signaling.
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Claims 10
Claim · model-written summary
COI1 physically associates with AtCUL1, AtRbx1 and ASK1 or ASK2 in SCF(COI1) complexes.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- COI1e0
- Outcome
- assemble ubiquitin-ligase complexese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase complexes, which we have designated SCF(COI1).”
Claim · model-written summary
COI1(E22A) abolished complex formation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- COI1(E22A)e0
- Gene
- COI1(E22A)e0
- Outcome
- formation of the SCF(COI1) complexese0
- Direction
- abolishese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COI1(E22A), a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the SCF(COI1) complexes and results in loss of the JA response.”
t0“Arabidopsis”
Claim · model-written summary
COI1(E22A) caused loss of JA response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- COI1(E22A)e0
- Gene
- COI1(E22A)e0
- Outcome
- JA responsee0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“COI1(E22A), a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the SCF(COI1) complexes and results in loss of the JA response.”
t0“Arabidopsis”
Claim · model-written summary
AtRbx1 interference reduced AtRbx1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- AtRbx1 double-stranded RNA-mediated genetic interferencee0
- Gene
- AtRbx1e0
- Outcome
- AtRbx1 expressione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression.”
t0“Arabidopsis”
Claim · model-written summary
AtRbx1 interference affected JA-inducible gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- AtRbx1 double-stranded RNA-mediated genetic interferencee0
- Gene
- AtRbx1e0
- Outcome
- JA-inducible gene expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression.”
t0“Arabidopsis”
Claim · model-written summary
AtCUL1 in SCF(COI1) is modified in planta.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtCUL1e0
- Outcome
- modified in plantae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the AtCUL1 component of SCF(COI1) complexes is modified in planta”
t0“Arabidopsis”
Claim · model-written summary
AXR1 mutations decreased modified AtCUL1 abundance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutations in AXR1e0
- Gene
- AXR1e0
- Outcome
- abundance of the modified AtCUL1e0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in AXR1 decrease the abundance of the modified AtCUL1 of SCF(COI1)”
t0“Arabidopsis”
Claim · model-written summary
AXR1 mutations reduced JA response.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutations in AXR1e0
- Gene
- AXR1e0
- Outcome
- JA responsee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in AXR1 decrease the abundance of the modified AtCUL1 of SCF(COI1) and lead to a reduction in JA response.”
t0“Arabidopsis”
Claim · model-written summary
axr1 and coi1 mutations had synergistic interaction in the double mutant.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- axr1 and coi1e0
- Outcome
- synergistic genetic interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the axr1 and coi1 mutations display a synergistic genetic interaction in the double mutant.”
t0“Arabidopsis”
Claim · model-written summary
Authors infer that JA response depends on SCF(COI1) and AXR1-dependent AtCUL1 modification.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtCUL1e0
- Outcome
- COI1-mediated JA responsee0
- Qualifier
- AXR1-dependent modificatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the COI1-mediated JA response is dependent on the SCF(COI1) complexes in Arabidopsis and that the AXR1-dependent modification of the AtCUL1 subunit of SCF(COI1) complexes is important for JA signaling.”
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