Abstract
The SUPPRESSOR OF rps4-RLD1 (SRFR1) gene was identified based on enhanced AvrRps4-triggered resistance in the naturally susceptible Arabidopsis accession RLD. No other phenotypic effects were recorded, and the extent of SRFR1 involvement in regulating effector-triggered immunity was unknown. Here we show that mutations in SRFR1 in the accession Columbia-0 (Col-0) lead to severe stunting and constitutive expression of the defense gene PR1. These phenotypes were temperature-dependent. A cross between srfr1-1 (RLD background) and srfr1-4 (Col-0) showed that stunting was caused by a recessive locus in Col-0. Mapping and targeted crosses identified the Col-0-specific resistance gene SNC1 as the locus that causes stunting. SRFR1 was proposed to function as a transcriptional repressor, and SNC1 is indeed overexpressed in srfr1-4. Interestingly, co-regulated genes in the SNC1 cluster are also upregulated in the srfr1-4 snc1-11 double mutant, indicating that the overexpression of SNC1 is not a secondary effect of constitutive defense activation. In addition, a Col-0 RPS4 mutant showed full susceptibility to bacteria expressing avrRps4 at 24°C but not at 22°C, while RLD susceptibility was not temperature-dependent. The rps4-2 snc1-11 double mutant showed increased, but not full, susceptibility at 22°C, indicating that additional cross-talk between resistance pathways may exist. Intriguingly, when transiently expressed in Nicotiana benthamiana, SRFR1, RPS4 and SNC1 are in a common protein complex in a cytoplasmic microsomal compartment. Our results highlight SRFR1 as a convergence point in at least a subset of TIR-NBS-LRR protein-mediated immunity in Arabidopsis. Based on the cross-talk evident from our results, they also suggest that reports of constitutive resistance phenotypes in Col-0 need to consider the possible involvement of SNC1.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
SRFR1 mutations in Col-0 caused severe stunting.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- Col-0e0
- Treatment
- mutations in SRFR1e0
- Gene
- SRFR1e0
- Outcome
- severe stuntinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in SRFR1 in the accession Columbia-0 (Col-0) lead to severe stunting”
t0“Arabidopsis”
Claim · model-written summary
SRFR1 mutations in Col-0 caused constitutive PR1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutations in SRFR1e0
- Gene
- PR1e0
- Outcome
- constitutive expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in SRFR1 in the accession Columbia-0 (Col-0) lead to severe stunting and constitutive expression of the defense gene PR1”
t0“Arabidopsis”
Claim · model-written summary
The stunting and PR1 expression phenotypes depended on temperature.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- phenotypese0
- Qualifier
- temperature-dependente0
Not reportedSettingDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These phenotypes were temperature-dependent”
t0“Arabidopsis”
Claim · model-written summary
A recessive Col-0 locus caused stunting.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Col-0e0
- Outcome
- stuntinge0
- Qualifier
- recessive locuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stunting was caused by a recessive locus in Col-0”
t0“Arabidopsis”
Claim · model-written summary
Mapping identified SNC1 as the Col-0 locus causing stunting.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SNC1e0
- Outcome
- stuntinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Col-0-specific resistance gene SNC1 as the locus that causes stunting”
t0“Arabidopsis”
Claim · model-written summary
SNC1 was overexpressed in srfr1-4.
- Taxon
- ArabidopsisArabidopsist0
- Material
- srfr1-4e0
- Gene
- SNC1e0
- Outcome
- overexpressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNC1 is indeed overexpressed in srfr1-4”
t0“Arabidopsis”
Claim · model-written summary
SNC1 cluster coregulated genes were upregulated in the double mutant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- srfr1-4 snc1-11 double mutante0
- Gene
- SNC1e0
- Outcome
- co-regulated genese0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-regulated genes in the SNC1 cluster are also upregulated in the srfr1-4 snc1-11 double mutant”
t0“Arabidopsis”
Claim · model-written summary
The Col-0 RPS4 mutant was fully susceptible to avrRps4-expressing bacteria at 24°C.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Col-0 RPS4 mutante0
- Gene
- RPS4e0
- Outcome
- full susceptibilitye0
- Qualifier
- 24°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Col-0 RPS4 mutant showed full susceptibility to bacteria expressing avrRps4 at 24°C but not at 22°C”
t0“Arabidopsis”
Claim · model-written summary
The Col-0 RPS4 mutant was not fully susceptible at 22°C.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Col-0 RPS4 mutante0
- Gene
- RPS4e0
- Outcome
- susceptibilitye0
- Qualifier
- not at 22°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Col-0 RPS4 mutant showed full susceptibility to bacteria expressing avrRps4 at 24°C but not at 22°C”
t0“Arabidopsis”
Claim · model-written summary
RLD susceptibility was temperature-independent.
- Taxon
- ArabidopsisArabidopsist0
- Material
- RLDe0
- Outcome
- susceptibilitye0
- Qualifier
- not temperature-dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RLD susceptibility was not temperature-dependent”
t0“Arabidopsis”
Claim · model-written summary
The rps4-2 snc1-11 double mutant had increased but incomplete susceptibility at 22°C.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rps4-2 snc1-11 double mutante0
- Outcome
- susceptibilitye0
- Direction
- increasede0
- Qualifier
- but not fulle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rps4-2 snc1-11 double mutant showed increased, but not full, susceptibility at 22°C”
t0“Arabidopsis”
Claim · model-written summary
SRFR1, RPS4 and SNC1 shared a cytoplasmic microsomal protein complex when expressed in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- SRFR1, RPS4 and SNC1e0
- Outcome
- common protein complexe0
- Qualifier
- cytoplasmic microsomal compartmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when transiently expressed in Nicotiana benthamiana, SRFR1, RPS4 and SNC1 are in a common protein complex in a cytoplasmic microsomal compartment”
Claim · model-written summary
The authors recommend considering SNC1 involvement when interpreting constitutive resistance in Col-0.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Col-0e0
- Gene
- SNC1e0
- Outcome
- consider the possible involvement of SNC1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reports of constitutive resistance phenotypes in Col-0 need to consider the possible involvement of SNC1”
t0“Arabidopsis”
The same questions in other papers
loss of function · molecular: expression · ArabidopsisPositive and negative results
37 papers in the same direction as this paper · 159 in the opposite direction
- negative
- negative
- negative
193 papers more in this group
See all 197 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.