Abstract
A salt‐tolerant line of alfalfa ( Medicago sativa L.) cells was selected for use in a comparative study examining the cellular traits associated with the capacity to tolerate salt. Suspension cultures of alfalfa cells were plated onto agarsolidified media containing 1% (w/v) NaCl. Within 2 months of exposure, more than 99% of these plated cells exhibited arrested growth and discoloration. A small number of cells exposed to salt, however, continued to grow and maintain a healthy appearance. These variants were subcultured onto media containing 1% NaCl every 3 to 4 weeks for 8 months. Several differences were observed between a salt‐selected cell line and a nonselected cell line from the original population in response to a gradient of NaCl concentrations in the nutrient media. Callus growth rates and ionic uptake by the cell lines differed. The selected line grew better than the unselected culture at high levels of NaCl, suggesting an increased NaCl tolerance. This selected line performed poorly in the absence of NaCl and the data suggest that it required a substantial amount of NaCl for optimal growth. A general shift towards a halophytic mode of salt tolerance within the salt‐selected cell line is suggested.
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Claims 8
Claim · model-written summary
More than 99% of plated alfalfa cells arrested growth and discolored after salt exposure.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- 1% (w/v) NaCle0
- Outcome
- arrested growth and discoloratione0
- Value
- more than 99%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suspension cultures of alfalfa cells were plated onto agarsolidified media containing 1% (w/v) NaCl. Within 2 months of exposure, more than 99% of these plated cells exhibited arrested growth and discoloration”
Claim · model-written summary
A small number of salt-exposed alfalfa cells continued growing and appeared healthy.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- salte0
- Outcome
- continued to grow and maintain a healthy appearancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A small number of cells exposed to salt, however, continued to grow and maintain a healthy appearance”
t0“Alfalfa”
Claim · model-written summary
Callus growth rates differed between selected and nonselected alfalfa cell lines across NaCl concentrations.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- Callus growth ratese0
- Direction
- differede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“Callus growth rates and ionic uptake by the cell lines differed”
Claim · model-written summary
Ionic uptake differed between selected and nonselected alfalfa cell lines.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- ionic uptakee0
- Direction
- differede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“Callus growth rates and ionic uptake by the cell lines differed”
Claim · model-written summary
The selected alfalfa line grew better than the unselected culture at high NaCl levels.
- Taxon
- AlfalfaMedicago sativat0
- Material
- selected linee0
- Treatment
- high levels of NaCle0
- Outcome
- grewe0
- Direction
- bettere0
- Comparator
- unselected culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“The selected line grew better than the unselected culture at high levels of NaCl”
Claim · model-written summary
The selected alfalfa line performed poorly without NaCl.
- Taxon
- AlfalfaMedicago sativat0
- Material
- selected linee0
- Outcome
- performed poorlye0
- Direction
- poorlye0
- Qualifier
- in the absence of NaCle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“This selected line performed poorly in the absence of NaCl”
Claim · model-written summary
The authors suggest the selected line requires substantial NaCl for optimal growth.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- NaCle0
- Outcome
- optimal growthe0
- Qualifier
- a substantial amount of NaCle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“the data suggest that it required a substantial amount of NaCl for optimal growth”
Claim · model-written summary
The authors suggest the salt-selected line shifted toward a halophytic mode of salt tolerance.
- Taxon
- AlfalfaMedicago sativat0
- Material
- salt‐selected cell linee0
- Outcome
- shift towards a halophytic mode of salt tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“A general shift towards a halophytic mode of salt tolerance within the salt‐selected cell line is suggested”
The same questions in other papers
sodium chloride · growth · Medicago sativa (alfalfa)Results differ
38 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.