Abstract
SUMMARY Callus cultures, established from cultivars of Medicago sativa L., Dupuits and Spredor2, were used to investigate salt‐induced changes in metabolism. With increase in NaCl concentration there was a progressive reduction in growth rate but this effect was ameliorated by increase in the Ca 2+ concentration. The concentration of Na + in the callus increased in response to NaCl in the medium but not proportionally, while the K + concentration was lowest in calli grown at the highest NaCl concentration. High Ca 2+ partially reversed the salt‐induced decrease in K + , so overall, the effect of Ca 2+ was to decrease the Na + /K + ratio. Proline concentration in the callus increased in response to NaCl. With callus cultures grown in the absence of supplemental NaCl there was no correlation between growth and proline concentration, whereas at the highest NaCl concentration used (150 mol m −3 ) there was a strong positive correlation between growth and proline concentration of individual calli. Increased Ca 2+ increased the proline concentration in callus of both cultivars, and at the highest salt concentration resulted in higher proline concentration and increased growth. It is suggested that proline accumulation is important for cell growth only when a certain level of salt‐stress is attained, and this level will depend on the presence or absence of other protectant mechanisms in the tissue.
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Claims 12
Claim · model-written summary
Increasing NaCl progressively reduced callus growth rate.
- Taxon
- Medicago sativa L.
Medicago sativat0 - Treatment
- NaCl concentratione0
- Outcome
- growth ratee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With increase in NaCl concentration there was a progressive reduction in growth rate”
t0“Medicago sativa L.”
Claim · model-written summary
Increasing calcium ameliorated the salt-induced reduction in growth rate.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- increase in the Ca 2+ concentratione0
- Outcome
- growth ratee1
- Direction
- amelioratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this effect was ameliorated by increase in the Ca 2+ concentration”
t0“growth, potassium nutrition, and proline accumulation in callus cultures of Medicago sativa L.”
e1“With increase in NaCl concentration there was a progressive reduction in growth rate”
Claim · model-written summary
Callus sodium concentration increased with medium NaCl, but not proportionally.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- NaCl in the mediume0
- Outcome
- concentration of Na + in the calluse0
- Direction
- increasede0
- Qualifier
- not proportionallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentration of Na + in the callus increased in response to NaCl in the medium but not proportionally”
t0“Medicago sativa L.”
Claim · model-written summary
Callus potassium concentration was lowest at the highest NaCl concentration.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- highest NaCl concentratione0
- Outcome
- K + concentratione0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the K + concentration was lowest in calli grown at the highest NaCl concentration”
t0“Medicago sativa L.”
Claim · model-written summary
High calcium partially reversed the salt-induced decrease in callus potassium.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- High Ca 2+e0
- Outcome
- K +e0
- Direction
- partially reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High Ca 2+ partially reversed the salt‐induced decrease in K +”
t0“Medicago sativa L.”
Claim · model-written summary
Calcium decreased the callus sodium-to-potassium ratio.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- Ca 2+e0
- Outcome
- Na + /K + ratioe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effect of Ca 2+ was to decrease the Na + /K + ratio”
t0“Medicago sativa L.”
Claim · model-written summary
Callus proline concentration increased with NaCl.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- NaCle0
- Outcome
- Proline concentration in the calluse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proline concentration in the callus increased in response to NaCl”
t0“Medicago sativa L.”
Claim · model-written summary
Without supplemental NaCl, growth and proline concentration were uncorrelated.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- absence of supplemental NaCle0
- Compound
- prolinee0
- Outcome
- growthe0
- Direction
- no correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the absence of supplemental NaCl there was no correlation between growth and proline concentration”
t0“Medicago sativa L.”
Claim · model-written summary
At 150 mol m −3 NaCl, growth and proline concentration had a strong positive correlation.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- highest NaCl concentratione0
- Compound
- prolinee0
- Outcome
- growthe0
- Direction
- strong positive correlatione0
- Value
- 150 mol m −3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the highest NaCl concentration used (150 mol m −3 ) there was a strong positive correlation between growth and proline concentration of individual calli”
t0“Medicago sativa L.”
Claim · model-written summary
Increasing calcium raised callus proline concentration in both cultivars.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- Increased Ca 2+e0
- Outcome
- proline concentration in calluse0
- Direction
- increasede0
- Qualifier
- both cultivarse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased Ca 2+ increased the proline concentration in callus of both cultivars”
t0“Medicago sativa L.”
Claim · model-written summary
At the highest salt concentration, increased calcium raised callus growth.
- Taxon
- Medicago sativa L.Medicago sativat0
- Treatment
- Ca 2+e1
- Outcome
- growthe0
- Direction
- increasede0
- Qualifier
- highest salt concentratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the highest salt concentration resulted in higher proline concentration and increased growth”
t0“calcium chlorides and light on growth, potassium nutrition, and proline accumulation in callus cultures of Medicago sativa L.”
e1“this effect was ameliorated by increase in the Ca 2+ concentration”
Claim · model-written summary
Proline accumulation may support cell growth only above a salt-stress threshold, depending on other protective mechanisms.
- Taxon
- Medicago sativa L.Medicago sativat0
- Compound
- prolinee0
- Outcome
- cell growthe0
- Qualifier
- only when a certain level of salt‐stress is attainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is suggested that proline accumulation is important for cell growth only when a certain level of salt‐stress is attained, and this level will depend on the presence or absence of other protectant mechanisms in the tissue”
t0“Medicago sativa L.”
The same questions in other papers
sodium chloride · growth · Medicago sativa (alfalfa)Results differ
38 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
39 papers more in this group
See all 43 papers →calcium(2+) · growth · Medicago sativa (alfalfa)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.