Abstract
The expression of asparagine synthetase (AS; EC 6.3.5.4) in response to externally supplied nitrogen was investigated with respect to enzyme activity and protein levels as detected immunologically in rice (Oryza sativa) seedlings. The asparagine content was very low in leaves and roots of nitrogen-starved rice plants but increased significantly after the supply of 1 mM NH4+ to the nutrient solution. While neither AS activity nor AS protein could be detected in leaves and roots prior to the supply of nitrogen, levels became detectable in roots but not in leaves within 12 h of the supply of 1 mM NH4+ or 10 mM glutamine. Other nitrogen compounds, such as nitrate, glutamate, aspartate and asparagine had no effect. Methionine sulfoximine completely inhibited the NH4+-induced accumulation of AS protein but did not affect the glutamine-induced accumulation of the enzyme. The results suggested that glutamine or glutamine-derived metabolites regulate AS expression in rice roots.
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Claims 8
Claim · model-written summary
Supplying NH4+ significantly increased asparagine content in leaves and roots of nitrogen-starved rice.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- leaves and rootse0
- Treatment
- supply of 1 mM NH4+e0
- Outcome
- asparagine contente0
- Direction
- increasede0
- Comparator
- nitrogen-starvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The asparagine content was very low in leaves and roots of nitrogen-starved rice plants but increased significantly after the supply of 1 mM NH4+ to the nutrient solution.”
Claim · model-written summary
NH4+ made AS protein and activity detectable in rice roots within 12 h.
- Taxon
- riceOryza sativat0
- Organ / stage
- rootse0
- Treatment
- supply of 1 mM NH4+e0
- Outcome
- levels became detectable in rootse0
- Value
- 12 he0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels became detectable in roots but not in leaves within 12 h of the supply of 1 mM NH4+ or 10 mM glutamine.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Glutamine made AS protein and activity detectable in rice roots within 12 h.
- Taxon
- riceOryza sativat0
- Organ / stage
- rootse0
- Treatment
- 10 mM glutaminee0
- Outcome
- levels became detectable in rootse0
- Value
- 12 he0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels became detectable in roots but not in leaves within 12 h of the supply of 1 mM NH4+ or 10 mM glutamine.”
t0“rice (Oryza sativa)”
Claim · model-written summary
AS remained undetectable in leaves after NH4+ supply.
- Taxon
- riceOryza sativat0
- Organ / stage
- leavese0
- Treatment
- supply of 1 mM NH4+e0
- Outcome
- not in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“levels became detectable in roots but not in leaves within 12 h of the supply of 1 mM NH4+ or 10 mM glutamine.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Nitrate, glutamate, aspartate and asparagine had no effect on AS expression.
- Taxon
- riceOryza sativat0
- Treatment
- nitrate, glutamate, aspartate and asparaginee0
- Outcome
- no effecte0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other nitrogen compounds, such as nitrate, glutamate, aspartate and asparagine had no effect.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Methionine sulfoximine completely inhibited NH4+-induced AS protein accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Methionine sulfoximinee0
- Gene
- AS proteine0
- Outcome
- NH4+-induced accumulation of AS proteine0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methionine sulfoximine completely inhibited the NH4+-induced accumulation of AS protein”
t0“rice (Oryza sativa)”
Claim · model-written summary
Methionine sulfoximine did not affect glutamine-induced enzyme accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Methionine sulfoximinee0
- Outcome
- glutamine-induced accumulation of the enzymee0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methionine sulfoximine completely inhibited the NH4+-induced accumulation of AS protein but did not affect the glutamine-induced accumulation of the enzyme.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Glutamine or its metabolites may regulate AS expression in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Compound
- glutamine or glutamine-derived metabolitese0
- Outcome
- AS expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggested that glutamine or glutamine-derived metabolites regulate AS expression in rice roots.”
The same questions in other papers
ammonium · molecular: metabolites · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
methionine sulfoximine · physiology: enzyme activity · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.