Abstract
Nitrate assimilation is an important process for the growth and development of plants. It is regulated at both transcritptional and posttranscriptional level by various factors including nitrogen metabolites. In the present study the effect of nitrogen metabolites on the regulation of nitrate reductase (NR) and nitrite reductase (NiR) in the shoots of 11-days hydroponically grown rice seedlings were studied. Both ammonium and glutamine caused a decrease in the activity of nitrate-induced NR while these metabolites had only a partial inhibitory or no effect on NiR. These metabolites had no effect on the enzyme activities in the absence of nitrate and on the nitrate uptake. Methionine sulfoximine, (MSX) an inhibitor of glutamine synthetase, was used to check whether the observed inhibitory effect on NR and NiR is due to ammonium itself or some other downstream metabolites. The results obtained show that while MSX had a partial inhibitory effect on NR. However it had no effect on NiR activities. On the other hand it increased the inhibitory effect of ammonium on nitrate reductase activity. Increase in inhibition to such an extent was not observed with NiR activity. These results indicate that the inhibition of NR and NiR activities was a direct effect of ammonium accumulation rather than an effect of its assimilation products.
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Claims 9
Claim · model-written summary
Ammonium reduced nitrate induced NR activity.
- Taxon
- rice
Oryza sativat0 - Treatment
- ammoniume0
- Gene
- NRe0
- Outcome
- activity of nitrate-induced NRe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Both ammonium and glutamine caused a decrease in the activity of nitrate-induced NR”
Claim · model-written summary
Glutamine reduced nitrate induced NR activity.
- Taxon
- riceOryza sativat0
- Treatment
- glutaminee0
- Gene
- NRe0
- Outcome
- activity of nitrate-induced NRe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Both ammonium and glutamine caused a decrease in the activity of nitrate-induced NR”
Claim · model-written summary
Ammonium and glutamine partially inhibited or did not affect NiR.
- Taxon
- riceOryza sativat0
- Treatment
- these metabolitese0
- Gene
- NiRe0
- Outcome
- NiRe0
- Direction
- partial inhibitory or no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“these metabolites had only a partial inhibitory or no effect on NiR”
Claim · model-written summary
These metabolites did not affect enzyme activity without nitrate or nitrate uptake.
- Taxon
- riceOryza sativat0
- Treatment
- These metabolitese0
- Outcome
- enzyme activities in the absence of nitrate and on the nitrate uptakee0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“These metabolites had no effect on the enzyme activities in the absence of nitrate and on the nitrate uptake”
Claim · model-written summary
MSX partially inhibited NR activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“MSX had a partial inhibitory effect on NR”
Claim · model-written summary
MSX did not affect NiR activity.
- Taxon
- riceOryza sativat0
- Treatment
- Methionine sulfoximinee1
- Gene
- NiRe0
- Outcome
- NiR activitiese0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“it had no effect on NiR activities”
e1“Methionine sulfoximine, (MSX) an inhibitor of glutamine synthetase”
Claim · model-written summary
MSX increased ammonium inhibition of nitrate reductase.
- Taxon
- riceOryza sativat0
- Treatment
- Methionine sulfoximinee1
- Gene
- nitrate reductasee0
- Outcome
- inhibitory effect of ammonium on nitrate reductase activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“it increased the inhibitory effect of ammonium on nitrate reductase activity”
e1“Methionine sulfoximine, (MSX) an inhibitor of glutamine synthetase”
Claim · model-written summary
MSX did not similarly increase ammonium inhibition of NiR.
- Taxon
- riceOryza sativat0
- Gene
- NiRe0
- Outcome
- NiR activitye0
- Direction
- not observede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Increase in inhibition to such an extent was not observed with NiR activity”
Claim · model-written summary
The authors attributed enzyme inhibition to ammonium accumulation rather than assimilation products.
- Taxon
- riceOryza sativat0
- Compound
- ammoniume0
- Outcome
- inhibition of NR and NiR activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“the inhibition of NR and NiR activities was a direct effect of ammonium accumulation rather than an effect of its assimilation products”
The same questions in other papers
metabolite · soil: biology · Oryza sativa (rice)Positive and negative results
2 in the opposite direction
- positive
- positive
methionine sulfoximine · physiology: enzyme activity · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- no effect
nitrogen · physiology: enzyme activity · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 28 in the opposite direction
- positive
- positive
- positive
32 papers more in this group
See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.