Abstract
Expression of a class I patatin cDNA clone, SK24-1, in Escherichia coli revealed that the cDNA clone possessed lipid acyl hydrolase (LAH) activity. Transformed potato plants were obtained via Agrobacterium-mediated transformation using the chimeric constructs containing the sense and antisense cDNA under the control cauliflower mosaic virus 35S (CaMV 35S) promoter. In some sense transformed plants, both sense patatin RNA and LAH activity were increased and further resulted in a significant increase of percentage of plantlets that formed microtubers and numbers of microtubers per plantlet in vitro. All antisense plants displayed a reduction in LAH activity. Both sense and antisense RNA could be detected in antisense plants, but transcripts of antisense RNA resulted in a reduction of endogenous sense RNA. Moreover, expression of antisense cDNA in some antisense transformed plants led to a significant decrease in the number of microtubers formed. These results suggest that SK24-1 was involved in regulating microtuber formation. Key words: Patatin, potato, Escherichia coli, sense RNA, antisense RNA
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Claims 7
Claim · model-written summary
Expression of the class I patatin cDNA SK24-1 in E. coli showed the clone has lipid acyl hydrolase (LAH) activity.
- Taxon
- potatoes
Solanum tuberosumt0 - Gene
- SK24-1e1
- Outcome
- possessed lipid acyl hydrolase (LAH) activitye1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Expression of a class I patatin cDNA clone, SK24-1, in Escherichia coli revealed that the cDNA clone possessed lipid acyl hydrolase (LAH) activity”
t0“transgenic potatoes”
Claim · model-written summary
In some sense-transformed potato plants both sense patatin RNA and LAH activity increased.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- sense transformed plantse1
- Outcome
- sense patatin RNA and LAH activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In some sense transformed plants, both sense patatin RNA and LAH activity were increased and further resulted in a significant increase of percentage of plantlets that formed microtubers and numbers of microtubers per plantlet in vitro”
t0“transgenic potatoes”
Claim · model-written summary
In some sense-transformed plants the percentage of plantlets forming microtubers and microtubers per plantlet in vitro increased significantly.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- sense transformed plantse1
- Outcome
- percentage of plantlets that formed microtubers and numbers of microtubers per plantlete1
- Direction
- increasee1
- Setting
- in vitroe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In some sense transformed plants, both sense patatin RNA and LAH activity were increased and further resulted in a significant increase of percentage of plantlets that formed microtubers and numbers of microtubers per plantlet in vitro”
t0“transgenic potatoes”
Claim · model-written summary
All antisense transformed plants showed reduced LAH activity.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- antisense plantse1
- Outcome
- LAH activitye1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“All antisense plants displayed a reduction in LAH activity”
t0“transgenic potatoes”
Claim · model-written summary
Antisense RNA transcripts reduced endogenous sense RNA in antisense plants.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- antisense RNAe1
- Outcome
- endogenous sense RNAe1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“transcripts of antisense RNA resulted in a reduction of endogenous sense RNA”
t0“transgenic potatoes”
Claim · model-written summary
In some antisense transformed plants antisense cDNA expression significantly decreased the number of microtubers formed.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- antisense cDNAe1
- Outcome
- number of microtubers formede1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“expression of antisense cDNA in some antisense transformed plants led to a significant decrease in the number of microtubers formed”
t0“transgenic potatoes”
Claim · model-written summary
The authors suggest SK24-1 is involved in regulating microtuber formation.
- Taxon
- potatoesSolanum tuberosumt0
- Gene
- SK24-1e1
- Outcome
- microtuber formatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results suggest that SK24-1 was involved in regulating microtuber formation”
t0“transgenic potatoes”
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