Abstract
Effects of age and orientation of the explant on callus induction and de novo shoot regeneration from cotyledonary leaf segments of Jatropha curcas were studied. The callus induction and shoot regeneration capacity of cotyledonary leaf segments were found significantly related to the age of the explants and their orientation in culture medium. The youngest explant, derived from the cotyledonary leaf of germinated seed induced the highest regeneration response as compared to one- and two-week-old explants. A gradient response with age of the explant was observed in percentage of callus induction, shoot regeneration from callus and the number of shoots per regenerating callus. The explants cultured with their abaxial side in medium showed significantly higher regeneration response. The youngest explant was found to be most amenable to Agrobacterium-mediated transformation as compared to older explants. The fact that callus induced from the edges of the explant followed by de novo shoot induction, and strong transient gus expression observed in the edges of the explant are significant for routine Agrobacterium-mediated transformation and generation of stable transgenic plants in J. curcas.
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Claims 9
Claim · model-written summary
Callus induction and shoot regeneration capacity were significantly related to explant age and orientation.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Organ / stage
- cotyledonary leaf segmentse0
- Treatment
- age of the explants and their orientation in culture mediume0
- Outcome
- callus induction and shoot regeneration capacitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“The callus induction and shoot regeneration capacity of cotyledonary leaf segments were found significantly related to the age of the explants and their orientation in culture medium.”
t0“Jatropha curcas L.”
Claim · model-written summary
The youngest explants had the highest regeneration response compared with one- and two-week-old explants.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- cotyledonary leafe0
- Treatment
- The youngest explante0
- Outcome
- regeneration responsee0
- Direction
- higheste0
- Comparator
- one- and two-week-old explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The youngest explant, derived from the cotyledonary leaf of germinated seed induced the highest regeneration response as compared to one- and two-week-old explants.”
t0“Jatropha curcas L.”
Claim · model-written summary
Callus induction percentage varied with explant age.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- explante0
- Outcome
- percentage of callus inductione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gradient response with age of the explant was observed in percentage of callus induction, shoot regeneration from callus and the number of shoots per regenerating callus.”
t0“Jatropha curcas L.”
Claim · model-written summary
Shoot regeneration from callus varied with explant age.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- explante0
- Outcome
- shoot regeneration from calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gradient response with age of the explant was observed in percentage of callus induction, shoot regeneration from callus and the number of shoots per regenerating callus.”
t0“Jatropha curcas L.”
Claim · model-written summary
Shoots per regenerating callus varied with explant age.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- explante0
- Outcome
- number of shoots per regenerating calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gradient response with age of the explant was observed in percentage of callus induction, shoot regeneration from callus and the number of shoots per regenerating callus.”
t0“Jatropha curcas L.”
Claim · model-written summary
Abaxial-side-down explants had significantly higher regeneration response.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- abaxial side in mediume0
- Outcome
- regeneration responsee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The explants cultured with their abaxial side in medium showed significantly higher regeneration response.”
t0“Jatropha curcas L.”
Claim · model-written summary
The youngest explants were most amenable to Agrobacterium-mediated transformation.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- The youngest explante0
- Interactor
- Agrobacterium
.jpg?width=60)
Agrobacteriume0other - Outcome
- Agrobacterium-mediated transformatione0
- Direction
- most amenablee0
- Comparator
- older explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The youngest explant was found to be most amenable to Agrobacterium-mediated transformation as compared to older explants.”
t0“Jatropha curcas L.”
Claim · model-written summary
Callus formed at explant edges and was followed by de novo shoot induction.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- explante0
- Outcome
- callus induced from the edges of the explant followed by de novo shoot inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus induced from the edges of the explant followed by de novo shoot induction”
t0“Jatropha curcas L.”
Claim · model-written summary
Strong transient gus expression was seen at explant edges.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- explante0
- Gene
- guse0
- Outcome
- transient gus expressione0
- Direction
- stronge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong transient gus expression observed in the edges of the explant”
t0“Jatropha curcas L.”
The same questions in other papers
in vitro · growth · Jatropha curcasPositive and negative results
24 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
- negative
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.