Abstract
Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes and ovule development. It was first isolated from tomato plants and has been successfully overexpressed in these plants using the Cauliflower Mosaic Virus (CaMV) 35S promoter. In this experiment, plant transformation was performed on J. curcas as the target. Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants. PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.
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Claims 3
Claim · model-written summary
Callus formation was good in treated and control Jatropha explants.
- Taxon
- Jatropha curcas
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Jatropha curcast0 - Outcome
- Explant callus formatione0
- Comparator
- control and treated samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants.”
t0“Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L.”
Claim · model-written summary
Shoot formation decreased dramatically in transformed Jatropha explants.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- treated explantse0
- Outcome
- shoot formatione0
- Direction
- decreased dramaticallye0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants.”
t0“Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L.”
Claim · model-written summary
PCR indicated IMA gene insertion into Jatropha, with a 500 bp gene size.
- Taxon
- J. curcasJatropha curcase0
- Gene
- IMA genese0
- Outcome
- insertede0
- Value
- 500 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.”
The same questions in other papers
in vitro · growth · Jatropha curcasPositive and negative results
1 paper in the same direction as this paper · 25 in the opposite direction
- positive
- positive
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