Abstract
The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica, represents a unique resource for the study of freeze tolerance mechanisms. We have previously characterized a multi-gene family in D. antarctica encoding ice recrystallization inhibition proteins (IRIPs) whose transcript levels are responsive to cold acclimation, and whose products confer ice recrystallization inhibition (RI) activity that can account for activity seen in cold acclimated plants. We used molecular and physiological analyses to investigate temporal responses of D. antarctica to cold acclimation and de-acclimation, and sub-zero acclimation. Quantitative profiling revealed that IRIP transcript levels significantly increased and decreased within hours of cold acclimation and de-acclimation, respectively, becoming up to 1000-fold more abundant in fully acclimated plants. Western analysis detected three major immuno-reactive bands whose pattern of accumulation mirrored that of transcript. These data correlated with the onset and decline of RI activity in acclimated and de-acclimated leaves. Plant survival-based testing revealed that cold acclimation enhanced freeze tolerance by 5 °C within 4 d, and that sub-zero acclimation conferred an additional 3 °C of tolerance. Thus, D. antarctica is highly responsive to temperature fluctuations, able to rapidly deploy IRIP based RI activity and enhance its freeze tolerance.
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Claims 6
Claim · model-written summary
Cold acclimation significantly increased IRIP transcript levels within hours, up to 1000-fold in fully acclimated plants.
- Taxon
- Deschampsia antarctica
Deschampsia antarcticae0 - Treatment
- cold acclimatione1
- Gene
- IRIPe1
- Outcome
- IRIP transcript levelse1
- Direction
- increasede1
- Value
- 1000-folde1
- Qualifier
- within hourse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e1“Quantitative profiling revealed that IRIP transcript levels significantly increased and decreased within hours of cold acclimation and de-acclimation, respectively, becoming up to 1000-fold more abundant in fully acclimated plants”
Claim · model-written summary
IRIP transcript levels decreased within hours of de-acclimation.
- Taxon
- Deschampsia antarcticaDeschampsia antarcticae0
- Treatment
- de-acclimatione1
- Gene
- IRIPe1
- Outcome
- IRIP transcript levelse1
- Direction
- decreasede1
- Qualifier
- within hourse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e1“Quantitative profiling revealed that IRIP transcript levels significantly increased and decreased within hours of cold acclimation and de-acclimation, respectively, becoming up to 1000-fold more abundant in fully acclimated plants”
Claim · model-written summary
The onset and decline of RI activity correlated with the IRIP transcript pattern in acclimated and de-acclimated leaves.
- Taxon
- Deschampsia antarcticaDeschampsia antarcticae0
- Organ / stage
- leavese3
- Outcome
- RI activitye3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e3“These data correlated with the onset and decline of RI activity in acclimated and de-acclimated leaves”
Claim · model-written summary
Three major immuno-reactive bands accumulated in a pattern that mirrored the IRIP transcript.
- Taxon
- Deschampsia antarcticaDeschampsia antarcticae0
- Outcome
- immuno-reactive bandse2
- Value
- three majore2
- Qualifier
- pattern of accumulation mirrored that of transcripte2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e2“Western analysis detected three major immuno-reactive bands whose pattern of accumulation mirrored that of transcript”
Claim · model-written summary
Cold acclimation enhanced freeze tolerance by 5 °C within 4 d.
- Taxon
- Deschampsia antarcticaDeschampsia antarcticae0
- Treatment
- cold acclimatione4
- Outcome
- freeze tolerancee4
- Direction
- enhancede4
- Value
- 5 °Ce4
- Qualifier
- within 4 de4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e4“Plant survival-based testing revealed that cold acclimation enhanced freeze tolerance by 5 °C within 4 d, and that sub-zero acclimation conferred an additional 3 °C of tolerance”
Claim · model-written summary
Sub-zero acclimation increased freeze tolerance by an additional 3 °C relative to cold acclimation.
- Taxon
- Deschampsia antarcticaDeschampsia antarcticae0
- Treatment
- sub-zero acclimatione4
- Outcome
- freeze tolerancee4
- Direction
- increaseinferrede4
- Value
- 3 °Ce4
- Qualifier
- an additionale4
- Comparator
- cold acclimatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cryophilic Antarctic hair grass, Deschampsia antarctica E. Desv., one of two higher plants indigenous to Antarctica”
e4“Plant survival-based testing revealed that cold acclimation enhanced freeze tolerance by 5 °C within 4 d, and that sub-zero acclimation conferred an additional 3 °C of tolerance”
The same questions in other papers
cold · molecular: expression · Deschampsia antarctica (Antarctic hairgrass)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.