Abstract
Few plant species can survive in Antarctica, the harshest environment for living organisms. Deschampsia antarctica is the only natural grass species to have adapted to and colonized the maritime Antarctic. To investigate the molecular mechanism of the Antarctic adaptation of this plant, we identified and characterized D. antarctica C-repeat binding factor 4 (DaCBF4), which belongs to monocot CBF group IV. The transcript level of DaCBF4 in D. antarctica was markedly increased by cold and dehydration stress. To assess the roles of DaCBF4 in plants, we generated a DaCBF4-overexpressing transgenic rice plant (Ubi:DaCBF4) and analyzed its abiotic stress response phenotype. Ubi:DaCBF4 displayed enhanced tolerance to cold stress without growth retardation under any condition compared to wild-type plants. Because the cold-specific phenotype of Ubi:DaCBF4 was similar to that of Ubi:DaCBF7 (Byun et al., 2015), we screened for the genes responsible for the improved cold tolerance in rice by selecting differentially regulated genes in both transgenic rice lines. By comparative transcriptome analysis using RNA-seq, we identified 9 and 15 genes under normal and cold-stress conditions, respectively, as putative downstream targets of the two D. antarctica CBFs. Overall, our results suggest that Antarctic hairgrass DaCBF4 mediates the cold-stress response of transgenic rice plants by adjusting the expression levels of a set of stress-responsive genes in transgenic rice plants. Moreover, selected downstream target genes will be useful for genetic engineering to enhance the cold tolerance of cereal plants, including rice.
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Claims 9
Claim · model-written summary
DaCBF4 was identified as a monocot CBF group IV factor in D. antarctica.
- Taxon
- D. antarctica
Deschampsia antarcticae0 - Gene
- DaCBF4e0
- Outcome
- belongs to monocot CBF group IVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified and characterized D. antarctica C-repeat binding factor 4 (DaCBF4), which belongs to monocot CBF group IV”
Claim · model-written summary
Cold stress markedly increased DaCBF4 transcript level in D. antarctica.
- Taxon
- D. antarcticaDeschampsia antarcticae0
- Treatment
- colde0
- Gene
- DaCBF4e0
- Outcome
- transcript level of DaCBF4e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of DaCBF4 in D. antarctica was markedly increased by cold and dehydration stress”
Claim · model-written summary
Dehydration stress markedly increased DaCBF4 transcript level in D. antarctica.
- Taxon
- D. antarcticaDeschampsia antarcticae0
- Treatment
- dehydration stresse0
- Gene
- DaCBF4e0
- Outcome
- transcript level of DaCBF4e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of DaCBF4 in D. antarctica was markedly increased by cold and dehydration stress”
Claim · model-written summary
Ubi:DaCBF4 rice showed enhanced cold tolerance relative to wild type.
- Taxon
- Rice
Oryza sativat0 - Material
- Ubi:DaCBF4e0
- Treatment
- cold stresse0
- Outcome
- tolerance to cold stresse0
- Direction
- enhancede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ubi:DaCBF4 displayed enhanced tolerance to cold stress without growth retardation under any condition compared to wild-type plants”
t0“Identification of Rice Genes”
Claim · model-written summary
Ubi:DaCBF4 rice showed no growth retardation relative to wild type under any condition.
- Taxon
- RiceOryza sativat0
- Material
- Ubi:DaCBF4e0
- Treatment
- cold stresse0
- Outcome
- growth retardatione0
- Direction
- withoute0
- Qualifier
- under any conditione0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ubi:DaCBF4 displayed enhanced tolerance to cold stress without growth retardation under any condition compared to wild-type plants”
t0“Identification of Rice Genes”
Claim · model-written summary
Comparative RNA-seq identified 9 putative downstream target genes under normal conditions.
- Taxon
- RiceOryza sativat0
- Outcome
- putative downstream targetse0
- Value
- 9e0
- Qualifier
- normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified 9 and 15 genes under normal and cold-stress conditions, respectively, as putative downstream targets of the two D. antarctica CBFs”
t0“Identification of Rice Genes”
Claim · model-written summary
Comparative RNA-seq identified 15 putative downstream target genes under cold stress.
- Taxon
- RiceOryza sativat0
- Outcome
- putative downstream targetse0
- Value
- 15e0
- Qualifier
- cold-stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified 9 and 15 genes under normal and cold-stress conditions, respectively, as putative downstream targets of the two D. antarctica CBFs”
t0“Identification of Rice Genes”
Claim · model-written summary
The authors propose DaCBF4 mediates cold response in transgenic rice by adjusting stress-responsive gene expression.
- Taxon
- riceOryza sativae0
- Gene
- DaCBF4e0
- Outcome
- cold-stress responsee0
- Qualifier
- adjusting the expression levels of a set of stress-responsive genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antarctic hairgrass DaCBF4 mediates the cold-stress response of transgenic rice plants by adjusting the expression levels of a set of stress-responsive genes in transgenic rice plants”
Claim · model-written summary
The authors suggest the selected downstream targets are useful for engineering cold-tolerant cereal plants.
- Taxon
- riceOryza sativae0
- Outcome
- genetic engineering to enhance the cold tolerance of cereal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“selected downstream target genes will be useful for genetic engineering to enhance the cold tolerance of cereal plants, including rice”
The same questions in other papers
cold · molecular: expression · Deschampsia antarctica (Antarctic hairgrass)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.