Abstract
BACKGROUND AND AIMS: Abscisic acid (ABA) plays crucial roles in plants' responses to abiotic stresses. ABA 8'-hydroxylation controlled by CYP707A genes has been well studied in Arabidopsis and rice, but not in legumes. The aims of the present study were to identify and functionally analyse the soybean CYP707A gene family, and to explore their expression patterns under dehydration and salt stresses. METHODS: A complementation experiment was employed to verify the function of soybean CYP707A1a in ABA catabolism. Genomic and cDNA sequences of other soybean CYP707A genes were isolated from the Phytozome database based on soybean CYP707A1a. The structure and phylogenetic relationship of this gene family was further analysed. The expression patterns of soybean CYP707A genes under dehydration and salt stress were analysed via quantitative real-time PCR. KEY RESULTS: Over-expression of GmCYP707A1a in the atcyp707a2 T-DNA insertion mutant decreased its sensitivity to ABA, indicating that GmCYP707A1a indeed functions as an ABA 8'-hydroxylase in higher plants. The soybean genome contains ten CYP707A genes. Gene structure and phylogenetic analysis showed high conservation of ten GmCYP707A genes to the other CYP707A genes from monocots and dicots. Seed imbibition induced expression of A1a, A1b, A2a, A2b, A2c, A3a and A5 in embryo, and expression of A1a, A1b, A2a and A2b in cotyledon. Dehydration induced expression of A1a, A1b, A2b, A2c, A3a, A3b, A4a, A4b and A5 both in roots and in leaves, whereas rehydration stimulated transcription of A2a, A2b, A3b, A4a and A5 in roots, and only A3b and A5 in leaves. Expression of all soybean CYP707A genes was induced either by short- or by long-term salt stress. CONCLUSIONS: The first biological evidence is provided that GmCYP7071a encodes an ABA 8'-hydroxylase through transgenic studies. Ten soybean GmCYP707A genes were identified, most of them expressed in multiple soybean tissues, and were induced by imbibition, dehydration and salinity.
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Claims 10
Claim · model-written summary
Overexpression of GmCYP707A1a decreased ABA sensitivity in an atcyp707a2 mutant.
- Material
- atcyp707a2 T-DNA insertion mutante0
- Treatment
- Over-expression of GmCYP707A1ae0
- Gene
- GmCYP707A1ae0
- Outcome
- sensitivity to ABAe0
- Direction
- decreasede0
Not reportedTaxonComparator
Verbatim
e0“Over-expression of GmCYP707A1a in the atcyp707a2 T-DNA insertion mutant decreased its sensitivity to ABA”
Claim · model-written summary
GmCYP707A1a was inferred to function as an ABA 8-hydroxylase.
Not reportedTaxon
Verbatim
e0“indicating that GmCYP707A1a indeed functions as an ABA 8'-hydroxylase in higher plants.”
Claim · model-written summary
The soybean genome contains ten CYP707A genes.
- Taxon
- soybean
Glycine maxe0 - Outcome
- CYP707A genese0
- Value
- tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The soybean genome contains ten CYP707A genes.”
Claim · model-written summary
Ten GmCYP707A genes showed high structural and phylogenetic conservation with monocot and dicot CYP707A genes.
- Gene
- GmCYP707A genese0
- Outcome
- high conservatione0
- Comparator
- other CYP707A genes from monocots and dicotse0
Not reportedTaxon
Verbatim
e0“Gene structure and phylogenetic analysis showed high conservation of ten GmCYP707A genes to the other CYP707A genes from monocots and dicots.”
Claim · model-written summary
Seed imbibition induced seven named CYP707A genes in embryos.
- Organ / stage
- embryoe0
- Treatment
- Seed imbibitione0
- Outcome
- expression of A1a, A1b, A2a, A2b, A2c, A3a and A5e0
- Direction
- inducede0
Not reportedTaxonComparator
Verbatim
e0“Seed imbibition induced expression of A1a, A1b, A2a, A2b, A2c, A3a and A5 in embryo”
Claim · model-written summary
Seed imbibition induced four named CYP707A genes in cotyledons.
- Organ / stage
- cotyledone0
- Treatment
- Seed imbibitione1
- Outcome
- expression of A1a, A1b, A2a and A2be0
- Direction
- inducede1
Not reportedTaxonComparator
Verbatim
e0“expression of A1a, A1b, A2a and A2b in cotyledon”
e1“Seed imbibition induced expression of A1a, A1b, A2a, A2b, A2c, A3a and A5 in embryo”
Claim · model-written summary
Dehydration induced nine named CYP707A genes in roots and leaves.
- Organ / stage
- roots and in leavese0
- Treatment
- Dehydratione0
- Outcome
- expression of A1a, A1b, A2b, A2c, A3a, A3b, A4a, A4b and A5e0
- Direction
- inducede0
Not reportedTaxonComparator
Verbatim
e0“Dehydration induced expression of A1a, A1b, A2b, A2c, A3a, A3b, A4a, A4b and A5 both in roots and in leaves”
Claim · model-written summary
Rehydration stimulated five named CYP707A genes in roots.
- Organ / stage
- rootse0
- Treatment
- rehydratione0
- Outcome
- transcription of A2a, A2b, A3b, A4a and A5e0
- Direction
- stimulatede0
Not reportedTaxonComparator
Verbatim
e0“rehydration stimulated transcription of A2a, A2b, A3b, A4a and A5 in roots”
Claim · model-written summary
Rehydration stimulated A3b and A5 in leaves.
Not reportedTaxonComparator
Verbatim
e0“only A3b and A5 in leaves”
e1“rehydration stimulated transcription of A2a, A2b, A3b, A4a and A5 in roots”
Claim · model-written summary
Short or long salt stress induced expression of all soybean CYP707A genes.
- Taxon
- soybeanGlycine maxe0
- Treatment
- short- or by long-term salt stresse0
- Outcome
- Expression of all soybean CYP707A genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of all soybean CYP707A genes was induced either by short- or by long-term salt stress.”
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See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.