Abstract
The receptor for activated C kinase 1 (RACK1) is one member of the most important WD repeat-containing family of proteins found in all eukaryotes and is involved in multiple signaling pathways. However, compared with the progress in the area of mammalian RACK1, our understanding of the functions and molecular mechanisms of RACK1 in the regulation of plant growth and development is still in its infancy. In the present study, we investigated the roles of rice RACK1A gene (OsRACK1A) in controlling seed germination and its molecular mechanisms by generating a series of transgenic rice lines, of which OsRACK1A was either over-expressed or under-expressed. Our results showed that OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2. Inhibition of ABA biosynthesis had no enhancing effect on germination, whereas inhibition of ABA catabolism significantly suppressed germination. ABA inhibition on seed germination was almost fully recovered by exogenous H2O2 treatment. Quantitative analyses showed that endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1A-down regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines. Quantitative real-time PCR analyses showed that the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines. It is concluded that OsRACK1A positively regulates seed germination by controlling endogenous levels of ABA and H2O2 and their interaction.
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Claims 10
Claim · model-written summary
OsRACK1A positively regulated rice seed germination.
- Taxon
- rice
Oryza sativat0 - Gene
- OsRACK1Ae0
- Outcome
- seed germinatione0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRACK1A positively regulated seed germination”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
OsRACK1A negatively regulated germination responses to exogenous ABA.
- Taxon
- riceOryza sativat0
- Treatment
- exogenous ABAe0
- Gene
- OsRACK1Ae0
- Outcome
- responses of seed germinatione0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
OsRACK1A negatively regulated germination responses to exogenous H2O2.
- Taxon
- riceOryza sativat0
- Treatment
- H2O2e0
- Gene
- OsRACK1Ae0
- Outcome
- responses of seed germinatione0
- Direction
- negatively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRACK1A positively regulated seed germination and negatively regulated the responses of seed germination to both exogenous ABA and H2O2”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
Inhibiting ABA biosynthesis did not enhance rice germination.
- Taxon
- riceOryza sativat0
- Treatment
- Inhibition of ABA biosynthesise0
- Outcome
- germinatione0
- Direction
- no enhancing effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of ABA biosynthesis had no enhancing effect on germination”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
Inhibiting ABA catabolism significantly suppressed rice germination.
- Taxon
- riceOryza sativat0
- Treatment
- inhibition of ABA catabolisme0
- Outcome
- germinatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of ABA catabolism significantly suppressed germination”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
Exogenous H2O2 almost fully restored rice germination inhibited by ABA.
- Taxon
- riceOryza sativat0
- Treatment
- exogenous H2O2 treatmente0
- Outcome
- seed germinatione0
- Direction
- recoverede0
- Qualifier
- almost fullye0
- Comparator
- ABA inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA inhibition on seed germination was almost fully recovered by exogenous H2O2 treatment”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
Endogenous ABA was significantly higher in OsRACK1A down regulated lines than wildtype or up regulated lines.
- Taxon
- riceOryza sativat0
- Material
- OsRACK1A-down regulated transgenic linese0
- Outcome
- endogenous ABA levelse0
- Direction
- highere0
- Comparator
- wildtype or OsRACK1A-up regulated linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1A-down regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
H2O2 was significantly lower in OsRACK1A down regulated lines than wildtype or up regulated lines.
- Taxon
- riceOryza sativat0
- Material
- OsRACK1A-down regulated transgenic linese0
- Outcome
- H2O2 levelse0
- Direction
- lowere0
- Comparator
- wildtype or OsRACK1A-up regulated linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous ABA levels were significantly higher and H2O2 levels significantly lower in OsRACK1A-down regulated transgenic lines as compared with those in wildtype or OsRACK1A-up regulated lines”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
OsRbohs transcript levels were significantly lower in OsRACK1A down regulated lines.
- Taxon
- riceOryza sativat0
- Material
- OsRACK1A-down regulated transgenic linese0
- Gene
- OsRbohse0
- Outcome
- transcript levelse0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines”
t0“Rice (Oryza sativa, L.)”
Claim · model-written summary
RAmy1A and RAmy3D transcript levels were significantly lower in OsRACK1A down regulated lines.
- Taxon
- riceOryza sativat0
- Material
- OsRACK1A-down regulated transgenic linese0
- Gene
- RAmy1A and RAmy3De0
- Outcome
- transcript levelse0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of OsRbohs and amylase genes, RAmy1A and RAmy3D, were significantly lower in OsRACK1A-down regulated transgenic lines”
t0“Rice (Oryza sativa, L.)”
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