Abstract
The ERECT PANICLE 3 gene of rice encodes a peptide that exhibits more than 50% sequence identity with the Arabidopsis F-box protein HAWAIIAN SKIRT (HWS). Ectopic expression of the Os02g15950 coding sequence, driven by the HWS (At3g61950) promoter, rescued the hws-1 flower phenotype in Arabidopsis confirming that EP3 is a functional orthologue of HWS. In addition to displaying an erect inflorescence phenotype, loss-of-function mutants of Os02g15950 exhibited a decrease in leaf photosynthetic capacity and stomatal conductance. Analysis of a range of physiological and anatomical features related to leaf photosynthesis revealed no alteration in Rubisco content and no notable changes in mesophyll size or arrangement. However, both ep3 mutant plants and transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) gene exhibit smaller stomatal guard cells compared with their wild-type controls. This anatomical characteristic may account for the observed decrease in leaf photosynthesis and provides evidence that EP3 plays a role in regulating stomatal guard cell development.
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Claims 8
Claim · model-written summary
Rice EP3 functionally complemented the Arabidopsis hws-1 flower phenotype.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Ectopic expression of the Os02g15950 coding sequencee0
- Gene
- Os02g15950e0
- Outcome
- hws-1 flower phenotypee0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of the Os02g15950 coding sequence, driven by the HWS (At3g61950) promoter, rescued the hws-1 flower phenotype in Arabidopsis”
Claim · model-written summary
Os02g15950 loss-of-function mutants had erect inflorescences.
- Taxon
- rice
Oryza sativat0 - Material
- ep3t0
- Outcome
- erect inflorescence phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“displaying an erect inflorescence phenotype”
t0“erect panicle 3 (ep3) mutant of rice”
Claim · model-written summary
Os02g15950 loss-of-function mutants had lower leaf photosynthetic capacity and stomatal conductance.
- Taxon
- riceOryza sativat0
- Material
- loss-of-function mutants of Os02g15950e0
- Outcome
- leaf photosynthetic capacity and stomatal conductancee0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss-of-function mutants of Os02g15950 exhibited a decrease in leaf photosynthetic capacity and stomatal conductance”
t0“mutant of rice”
Claim · model-written summary
Rubisco content was unaltered in the mutants.
- Taxon
- riceOryza sativat0
- Outcome
- Rubisco contente0
- Direction
- no alteratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no alteration in Rubisco content”
t0“mutant of rice”
Claim · model-written summary
Mesophyll size and arrangement did not notably change.
- Taxon
- riceOryza sativat0
- Outcome
- mesophyll size or arrangemente0
- Direction
- no notable changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no notable changes in mesophyll size or arrangement”
t0“mutant of rice”
Claim · model-written summary
ep3 mutant plants had smaller guard cells than wild-type controls.
- Taxon
- riceOryza sativat0
- Material
- ep3 mutant plantse0
- Outcome
- stomatal guard cellse0
- Direction
- smallere0
- Comparator
- wild-type controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ep3 mutant plants and transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) gene exhibit smaller stomatal guard cells compared with their wild-type controls”
t0“mutant of rice”
Claim · model-written summary
Os02g15950 T-DNA insertion lines had smaller guard cells than wild-type controls.
- Taxon
- riceOryza sativat0
- Material
- transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) genee0
- Outcome
- stomatal guard cellse0
- Direction
- smallere0
- Comparator
- wild-type controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ep3 mutant plants and transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) gene exhibit smaller stomatal guard cells compared with their wild-type controls”
t0“mutant of rice”
Claim · model-written summary
Smaller guard cells may account for decreased leaf photosynthesis.
- Taxon
- riceOryza sativat0
- Outcome
- leaf photosynthesise0
- Direction
- decreasee0
- Qualifier
- This anatomical characteristice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This anatomical characteristic may account for the observed decrease in leaf photosynthesis”
t0“mutant of rice”