Abstract
The successful regeneration of Jatropha curcas requires easily reproducible methods. Therefore, this study was undertaken to evaluate the effects of sources of explants, growth regulators, coconut water, nitrogen and flask sealing on the quality and quantity of shoots derived from leaf segments of Jatropha curcas after in vitro manipulation. The highest morphogenetic responses, 4.8 and 4.56 (numbers of adventitious shoots), were obtained from leaf cotyledon plantlets cultured in half-strength MS medium in the presence of BAP separately or combined with IAA (at a concentration of 4.4 muM of BAP + 2.8 muM of IAA). Enhanced shoot multiplication rates of 2.07 and 1.84 were observed in MS medium without coconut water and with the flask sealed with plastic film or a polypropylene lid with a filter, respectively. This result demonstrated the positive effects of minimum air circulation between the internal and external environments of the culture medium during regeneration. Concentrations of KNO3 and NH4NO3 between 50 to 100 % in MS medium favored the induction of callus and shoot formation at the 60th day of culture. Observations using a scanning electron microscope permitted visualization of dedifferentiated cells, and the reinitiation of meristematic activities resulted in the formation of a unipolar structure called a vegetative primordium. From these results, it is possible to conclude that regeneration efficiency (quality and quantity of material regenerated) in Jatropha curcas can be increased with the use of balanced medium constituents, growth regulators and modifications of culture conditions that include the absence of light.
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Claims 7
Claim · model-written summary
Cotyledon leaf explants on half-strength MS medium with BAP produced the highest adventitious shoot response, 4.8.
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Organ / stage
- leaf cotyledon plantletse0
- Treatment
- BAP separatelye0
- Outcome
- numbers of adventitious shootse0
- Direction
- higheste0
- Value
- 4.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“The highest morphogenetic responses, 4.8 and 4.56 (numbers of adventitious shoots), were obtained from leaf cotyledon plantlets cultured in half-strength MS medium in the presence of BAP separately or combined with IAA (at a concentration of 4.4 muM of BAP + 2.8 muM of IAA).”
t0“Jatropha curcas”
Claim · model-written summary
Cotyledon leaf explants with BAP and IAA yielded 4.56 adventitious shoots.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- leaf cotyledon plantletse0
- Treatment
- 4.4 muM of BAP + 2.8 muM of IAAe0
- Outcome
- numbers of adventitious shootse0
- Direction
- higheste0
- Value
- 4.56e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest morphogenetic responses, 4.8 and 4.56 (numbers of adventitious shoots), were obtained from leaf cotyledon plantlets cultured in half-strength MS medium in the presence of BAP separately or combined with IAA (at a concentration of 4.4 muM of BAP + 2.8 muM of IAA).”
t0“Jatropha curcas”
Claim · model-written summary
MS medium without coconut water and plastic film sealing gave a shoot multiplication rate of 2.07.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- MS medium without coconut water and with the flask sealed with plastic filme0
- Outcome
- shoot multiplication ratese0
- Direction
- Enhancede0
- Value
- 2.07e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced shoot multiplication rates of 2.07 and 1.84 were observed in MS medium without coconut water and with the flask sealed with plastic film or a polypropylene lid with a filter, respectively.”
t0“Jatropha curcas”
Claim · model-written summary
MS medium without coconut water and a filtered polypropylene lid gave a shoot multiplication rate of 1.84.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- a polypropylene lid with a filtere0
- Outcome
- shoot multiplication ratese0
- Direction
- Enhancede0
- Value
- 1.84e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced shoot multiplication rates of 2.07 and 1.84 were observed in MS medium without coconut water and with the flask sealed with plastic film or a polypropylene lid with a filter, respectively.”
t0“Jatropha curcas”
Claim · model-written summary
KNO3 and NH4NO3 at 50 to 100% in MS medium favored callus induction and shoot formation by day 60.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- Concentrations of KNO3 and NH4NO3 between 50 to 100 % in MS mediume0
- Outcome
- induction of callus and shoot formatione0
- Direction
- favorede0
- Qualifier
- 60th day of culturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concentrations of KNO3 and NH4NO3 between 50 to 100 % in MS medium favored the induction of callus and shoot formation at the 60th day of culture.”
t0“Jatropha curcas”
Claim · model-written summary
Microscopy showed dedifferentiated cells and resumed meristem activity forming a vegetative primordium.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- formation of a unipolar structure called a vegetative primordiume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Observations using a scanning electron microscope permitted visualization of dedifferentiated cells, and the reinitiation of meristematic activities resulted in the formation of a unipolar structure called a vegetative primordium.”
t0“Jatropha curcas”
Claim · model-written summary
The authors conclude that balanced medium, growth regulators and culture modifications including darkness can increase regeneration efficiency.
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- balanced medium constituents, growth regulators and modifications of culture conditions that include the absence of lighte0
- Outcome
- regeneration efficiencye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regeneration efficiency (quality and quantity of material regenerated) in Jatropha curcas can be increased with the use of balanced medium constituents, growth regulators and modifications of culture conditions that include the absence of light.”
The same questions in other papers
in vitro · molecular: expression · Jatropha curcasResults agree
1 paper in the same direction as this paper
- positive
in vitro · pest · Jatropha curcasResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.