Abstract
In the present investigation, in vitro propagation of Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog's (MS) medium supplemented with 20 μM N6-benzyl- adenine (BA) and 50 μ M adenine sulphate, in which cultures produced 8.2 ± 0.56 shoots per nodal explant with 3.0 cm length after 3-5 weeks. The rate of shoot multiplication was significantly enhanced after transfer to MS medium supplemented with 2.5 μ M 6-furfuryl amino purine (Kn), 0.5 μ M indole- 3-butyric acid (IBA) and 25 μ M adenine sulphate for 4 weeks. Internode explant segments of Jatropha curcas plants responded in vitro and formed callus tissue when cultured on Murashige-Skoog (MS) full strength nutrient medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D - 4 mg/L) and N6-Benzyl adenine (BA- 4 mg/L). The internode-derived callus tissues were found non-embryogenic and hence did not regenerate into shoot and root, respectively. The internode segments when cultured on MS (full strength) media supplemented with BA - 5 mg/L) were found to grow forming two to three buds. However, these shooting explants did not form roots upon hormonal regulation. On the contrary, endosperm tissue cultured on full strength MS media supplemented with 3 mg/L BA and 1 mg/L Indole-3-butyric acid (IBA) along with activated charcoal (100 mg/L) and ascorbic acid (50 mg/L) yielded simultaneous shooting and rooting response after four weeks of incubation.
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Claims 7
Claim · model-written summary
The stated medium initiated 8.2 ± 0.56 shoots per nodal explant.
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Treatment
- Murashige and Skoog's (MS) medium supplemented with 20 μM N6-benzyl- adenine (BA) and 50 μ M adenine sulphatee0
- Outcome
- shoots per nodal explante0
- Direction
- beste0
- Value
- 8.2 ± 0.56 shoots per nodal explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Axillary shoot bud proliferation was best initiated on Murashige and Skoog's (MS) medium supplemented with 20 μM N6-benzyl- adenine (BA) and 50 μ M adenine sulphate, in which cultures produced 8.2 ± 0.56 shoots per nodal explant with 3.0 cm length after 3-5 weeks.”
t0“Jatropha curcas”
Claim · model-written summary
Shoot multiplication was significantly enhanced on the transfer medium.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- MS medium supplemented with 2.5 μ M 6-furfuryl amino purine (Kn), 0.5 μ M indole- 3-butyric acid (IBA) and 25 μ M adenine sulphatee0
- Outcome
- rate of shoot multiplicatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rate of shoot multiplication was significantly enhanced after transfer to MS medium supplemented with 2.5 μ M 6-furfuryl amino purine (Kn), 0.5 μ M indole- 3-butyric acid (IBA) and 25 μ M adenine sulphate for 4 weeks.”
t0“Jatropha curcas”
Claim · model-written summary
Internode explants formed callus on the stated medium.
- Taxon
- Jatropha curcasJatropha curcase0
- Treatment
- Murashige-Skoog (MS) full strength nutrient medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D - 4 mg/L) and N6-Benzyl adenine (BA- 4 mg/L)e0
- Outcome
- formed callus tissuee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Internode explant segments of Jatropha curcas plants responded in vitro and formed callus tissue when cultured on Murashige-Skoog (MS) full strength nutrient medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D - 4 mg/L) and N6-Benzyl adenine (BA- 4 mg/L).”
Claim · model-written summary
Internode callus was nonembryogenic and failed to regenerate shoots or roots.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- non-embryogenic and hence did not regenerate into shoot and roote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The internode-derived callus tissues were found non-embryogenic and hence did not regenerate into shoot and root, respectively.”
t0“Jatropha curcas”
Claim · model-written summary
Internode segments formed two to three buds on BA medium.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- MS (full strength) media supplemented with BA - 5 mg/L)e0
- Outcome
- budse0
- Direction
- forminge0
- Value
- two to three budse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The internode segments when cultured on MS (full strength) media supplemented with BA - 5 mg/L) were found to grow forming two to three buds.”
t0“Jatropha curcas”
Claim · model-written summary
The shooting internode explants failed to root after hormonal regulation.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- did not form rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, these shooting explants did not form roots upon hormonal regulation.”
t0“Jatropha curcas”
Claim · model-written summary
Endosperm tissue yielded simultaneous shoot and root formation after four weeks.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- endosperm tissuee0
- Treatment
- full strength MS media supplemented with 3 mg/L BA and 1 mg/L Indole-3-butyric acid (IBA) along with activated charcoal (100 mg/L) and ascorbic acid (50 mg/L)e0
- Outcome
- simultaneous shooting and rooting responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endosperm tissue cultured on full strength MS media supplemented with 3 mg/L BA and 1 mg/L Indole-3-butyric acid (IBA) along with activated charcoal (100 mg/L) and ascorbic acid (50 mg/L) yielded simultaneous shooting and rooting response after four weeks of incubation.”
t0“Jatropha curcas”
The same questions in other papers
N-benzyladenine · growth · Jatropha curcasPositive and negative results
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- no effect
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adenine · pest · Jatropha curcasResults agree
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sulfate · pest · Jatropha curcasResults agree
1 paper in the same direction as this paper
- positive
ascorbic acid · growth · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
indole-3-butyric acid · pest · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
2,4-D · growth · Jatropha curcasResults agree
10 papers in the same direction as this paper
- positive
- positive
- positive
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See all 11 papers →indole-3-butyric acid · growth · Jatropha curcasResults agree
23 papers in the same direction as this paper
- positive
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See all 24 papers →8 of 10 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.