Abstract
SummaryAn appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants. Shoot regeneration from apical shoots, nodes, axillary bud-derived shoots, petioles and leaf explants were assessed on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) alone, or in combination with indole-3-yl-butyric acid (IBA). MS medium with 2.22 – 4.44 µM BA was effective for apical shoot culture, while 4.44 µM BA was suitable for node culture. MS medium with 2.22 µM BA and 0.049 µM IBA provided the best shoot proliferation from axillary bud-derived shoots. For petiole segments from leaves at the second, third and fourth nodes, the most effective growth regulator combination for callus induction during the first culture cycle, and subsequent shoot formation during the second culture cycle, was MS medium supplemented with 4.44 µM BA and 2.46 µM IBA. A combination of 8.88 µM BA and 4.90 µM IBA was suitable for callus induction and shoot formation from leaf segments at the second and third nodes of branches. For rooting, 4.90 µM IBA induced 85.71% rooting from shoots on basal rooting medium. The in vitro plantlets were acclimatised and transferred to potting mix, with 82% survival.
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Claims 7
Claim · model-written summary
MS medium with 2.22-4.44 µM BA was effective for apical shoot culture of Jatropha curcas.
- Taxon
- Jatropha curcas L.
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Jatropha curcase0 - Treatment
- MS medium with 2.22 – 4.44 µM BAe1
- Outcome
- apical shoot culturee1
- Direction
- effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“MS medium with 2.22 – 4.44 µM BA was effective for apical shoot culture”
Claim · model-written summary
MS medium with 4.44 µM BA was suitable for node culture.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Treatment
- 4.44 µM BAe1
- Outcome
- node culturee1
- Direction
- suitablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“4.44 µM BA was suitable for node culture”
Claim · model-written summary
MS with 2.22 µM BA and 0.049 µM IBA gave the best shoot proliferation from axillary bud-derived shoots.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Organ / stage
- axillary bud-derived shootse1
- Treatment
- MS medium with 2.22 µM BA and 0.049 µM IBAe1
- Outcome
- shoot proliferatione1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“MS medium with 2.22 µM BA and 0.049 µM IBA provided the best shoot proliferation from axillary bud-derived shoots”
Claim · model-written summary
For petiole segments, MS with 4.44 µM BA and 2.46 µM IBA was the most effective combination for callus induction and subsequent shoot formation.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Organ / stage
- petiole segmentse1
- Treatment
- MS medium supplemented with 4.44 µM BA and 2.46 µM IBAe1
- Outcome
- callus inductione1
- Direction
- most effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“For petiole segments from leaves at the second, third and fourth nodes, the most effective growth regulator combination for callus induction during the first culture cycle, and subsequent shoot formation during the second culture cycle, was MS medium supplemented with 4.44 µM BA and 2.46 µM IBA”
Claim · model-written summary
A combination of 8.88 µM BA and 4.90 µM IBA was suitable for callus induction and shoot formation from leaf segments.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Organ / stage
- leaf segmentse1
- Treatment
- 8.88 µM BA and 4.90 µM IBAe1
- Outcome
- callus induction and shoot formatione1
- Direction
- suitablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“A combination of 8.88 µM BA and 4.90 µM IBA was suitable for callus induction and shoot formation from leaf segments at the second and third nodes of branches”
Claim · model-written summary
4.90 µM IBA induced 85.71% rooting from shoots on basal rooting medium.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Treatment
- 4.90 µM IBAe1
- Outcome
- rootinge1
- Direction
- inducede1
- Value
- 85.71%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“4.90 µM IBA induced 85.71% rooting from shoots on basal rooting medium”
Claim · model-written summary
In vitro plantlets were acclimatised and transferred to potting mix with 82% survival.
- Taxon
- Jatropha curcas L.Jatropha curcase0
- Outcome
- survivale1
- Value
- 82%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants”
e1“The in vitro plantlets were acclimatised and transferred to potting mix, with 82% survival”
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.