Abstract
Callus cultures were initiated from leaf and hypocotyl explants isolated from 4 days old seedling of jatropha curcas L., on Murashige & Skoog (1962) basal medium supplemented with different growth regulator formulations including 2.4-D, BA, GA3, and coconut milk. Excellent growth of callus was obtained in medium supplemented with 0.5mg/L 2, 4-D alone and with 2% v/v coconut milk in gypocotyl explants. Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture then stabilized at a low growth rate. Calli cultured on this medium showed 8 fold increase in fresh weight by the fourth week of incubation. Callus was soft, friable, globular. lush green in color. Hypocotyl explant and 0.5mg/L 2, 4-D proved to be mose effective in inducing of callus on a large scale in short period of time. The friable green callus was then used for establishment of homogeneous and chlorophyllous suspension culture. Maximum growth of suspension culture was achieved in medium supplemented with 0.5mg/L 2, 4-D, with initial inoculum cell density of 1%. The growth rates of cells were initially slow but as the cultures proceeded, the growth increased significantly and accumulated a great amout of fresh weight (5fold) over a period of 21 days then the growth of cells was stable for 30 days. The fresh weight was balanced in terms of dry weight which almost corresponded to fresh weight. Total chlorophyll content in cell culture varied between 50.7 to 75.7ug/g FW with in growth cycle of these cultures.
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Claims 10
Claim · model-written summary
The 2,4-D and coconut milk culture condition yielded excellent callus growth from hypocotyl explants.
- Taxon
- JATROPHA CURCAS
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Jatropha curcast0 - Organ / stage
- gypocotyl explantse0
- Treatment
- 0.5mg/L 2, 4-D alone and with 2% v/v coconut milke0
- Outcome
- growth of calluse0
- Direction
- Excellente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Excellent growth of callus was obtained in medium supplemented with 0.5mg/L 2, 4-D alone and with 2% v/v coconut milk in gypocotyl explants.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Hypocotyl callus grew faster during days 7 to 30, then growth stabilized at a low rate.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Organ / stage
- hypocotyl explantse0
- Outcome
- grew fastere0
- Direction
- fastere0
- Qualifier
- during 7 to 30 days of culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture then stabilized at a low growth rate.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Callus fresh weight increased eightfold by week four.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- fresh weighte0
- Direction
- increasee0
- Value
- 8 folde0
- Qualifier
- by the fourth week of incubatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calli cultured on this medium showed 8 fold increase in fresh weight by the fourth week of incubation.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Callus was soft, friable, globular and green.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- Callus was soft, friable, globular. lush green in colore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was soft, friable, globular. lush green in color.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Hypocotyl explants with 0.5 mg/L 2,4-D were judged most effective for rapid large scale callus induction.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Organ / stage
- Hypocotyl explante0
- Treatment
- 0.5mg/L 2, 4-De0
- Outcome
- inducing of callus on a large scale in short period of timee0
- Direction
- mose effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hypocotyl explant and 0.5mg/L 2, 4-D proved to be mose effective in inducing of callus on a large scale in short period of time.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Friable green callus established homogeneous chlorophyllous suspension culture.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- homogeneous and chlorophyllous suspension culturee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The friable green callus was then used for establishment of homogeneous and chlorophyllous suspension culture.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Suspension culture growth was maximal with 0.5 mg/L 2,4-D and 1% initial cell density.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Treatment
- 0.5mg/L 2, 4-De0
- Outcome
- growth of suspension culturee0
- Direction
- Maximume0
- Qualifier
- initial inoculum cell density of 1%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum growth of suspension culture was achieved in medium supplemented with 0.5mg/L 2, 4-D, with initial inoculum cell density of 1%.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Cell fresh weight accumulated fivefold over 21 days, after which growth stabilized.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- fresh weighte0
- Direction
- increasede0
- Value
- 5folde0
- Qualifier
- over a period of 21 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth increased significantly and accumulated a great amout of fresh weight (5fold) over a period of 21 days then the growth of cells was stable for 30 days.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Dry weight almost corresponded to fresh weight.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- dry weighte0
- Qualifier
- almost corresponded to fresh weighte0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh weight was balanced in terms of dry weight which almost corresponded to fresh weight.”
t0“JATROPHA CURCAS”
Claim · model-written summary
Cell culture total chlorophyll content varied from 50.7 to 75.7 ug/g FW across the growth cycle.
- Taxon
- JATROPHA CURCASJatropha curcast0
- Outcome
- Total chlorophyll contente0
- Value
- 50.7 to 75.7ug/g FWe0
- Qualifier
- with in growth cycle of these culturese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total chlorophyll content in cell culture varied between 50.7 to 75.7ug/g FW with in growth cycle of these cultures.”
t0“JATROPHA CURCAS”
The same questions in other papers
2,4-D · growth · Jatropha curcasResults agree
10 papers in the same direction as this paper
- positive
- positive
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.