Abstract
.Using immature zygotic embryos as explants, we have developed an efficient method for somatic embryogenesis in three germplasm accessions collected from China, India and Indonesia. Indirect somatic embryogenesis was achieved when endosperm tissue and immature embryos between 0.5-1.0 cm in length were cultured in a medium with 2,4-D, preferably at 5-10 mg/l, followed by a shift to a hormone-free medium supplemented with glutamine and asparagine. Production of secondary embryos was improved by supplementing KNO3, glutamine and asparagine. 2,4-D (0.1-0.2 mg/l). PEG 8000 (5-10%) were essential for maintenance of embryogenic calli in liquid medium. Regeneration of soil-ready plants took as short as 3 months using the suspension cultures. Over 95% of the regenerated trees were able to flower and set seeds with no discernable morphological abnormality. This regeneration method is expected to facilitate the development of more efficient transformation system for Jatropha curcas.
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Claims 5
Claim · model-written summary
Indirect somatic embryogenesis was achieved with endosperm tissue and immature embryos under the specified culture sequence.
- Taxon
- Jatropha curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Organ / stage
- endosperm tissue and immature embryose0
- Treatment
- cultured in a medium with 2,4-D, preferably at 5-10 mg/l, followed by a shift to a hormone-free medium supplemented with glutamine and asparaginee0
- Outcome
- Indirect somatic embryogenesis was achievede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Indirect somatic embryogenesis was achieved when endosperm tissue and immature embryos between 0.5-1.0 cm in length were cultured in a medium with 2,4-D, preferably at 5-10 mg/l, followed by a shift to a hormone-free medium supplemented with glutamine and asparagine.”
t0“Jatropha curcas”
Claim · model-written summary
KNO3, glutamine and asparagine supplementation improved secondary embryo production.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- supplementing KNO3, glutamine and asparaginee0
- Outcome
- Production of secondary embryose0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Production of secondary embryos was improved by supplementing KNO3, glutamine and asparagine.”
t0“Jatropha curcas”
Claim · model-written summary
2,4-D and PEG 8000 were reported as essential to maintain embryogenic calli in liquid medium.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- 2,4-D (0.1-0.2 mg/l). PEG 8000 (5-10%)e0
- Outcome
- maintenance of embryogenic callie0
- Setting
- liquid mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2,4-D (0.1-0.2 mg/l). PEG 8000 (5-10%) were essential for maintenance of embryogenic calli in liquid medium.”
t0“Jatropha curcas”
Claim · model-written summary
Suspension cultures regenerated soil-ready plants in as little as three months.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- suspension culturese0
- Outcome
- Regeneration of soil-ready plantse0
- Value
- 3 monthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration of soil-ready plants took as short as 3 months using the suspension cultures.”
t0“Jatropha curcas”
Claim · model-written summary
More than 95% of regenerated trees flowered and set seeds without discernable morphological abnormality.
- Taxon
- Jatropha curcasJatropha curcast0
- Organ / stage
- regenerated treese0
- Outcome
- able to flower and set seeds with no discernable morphological abnormalitye0
- Value
- Over 95%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over 95% of the regenerated trees were able to flower and set seeds with no discernable morphological abnormality.”
t0“Jatropha curcas”
The same questions in other papers
glutamine · growth · Jatropha curcasResults agree
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- positive
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- positive
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- no effect
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.