Abstract
Jatropha curcas L. is monoecious with a low female-to-male ratio, which is one of the factors restricting its seed yield. Because the phytohormone cytokinins play an essential role in flower development, particularly pistil development, in this study, we elevated the cytokinin levels in J. curcas flowers through transgenic expression of a cytokinin biosynthetic gene (AtIPT4) from Arabidopsis under the control of a J. curcas orthologue of TOMATO MADS BOX GENE 6 (JcTM6) promoter that is predominantly active in flowers. As expected, the levels of six cytokinin species in the inflorescences were elevated, and flower development was modified without any alterations in vegetative growth. In the transgenic J. curcas plants, the flower number per inflorescence was significantly increased, and most flowers were pistil-predominantly bisexual, i.e., the flowers had a huge pistil surrounded with small stamens. Unfortunately, both the male and the bisexual flowers of transgenic J. curcas were infertile, which might have resulted from the continuously high expression of the transgene during flower development. However, the number and position of floral organs in the transgenic flowers were well defined, which suggested that the determinacy of the floral meristem was not affected. These results suggest that fine-tuning the endogenous cytokinins can increase the flower number and the female-to-male ratio in J. curcas.
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Claims 8
Claim · model-written summary
In transgenic Jatropha curcas, flower number per inflorescence was significantly increased by flower-specific cytokinin overproduction.
- Taxon
- Jatropha curcas
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Jatropha curcast0 - Treatment
- transgenic expression of a cytokinin biosynthetic gene (AtIPT4)e1
- Gene
- AtIPT4e1
- Outcome
- flower number per inflorescencee3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e1“through transgenic expression of a cytokinin biosynthetic gene (AtIPT4) from Arabidopsis under the control of a J. curcas orthologue of TOMATO MADS BOX GENE 6 (JcTM6) promoter”
e3“In the transgenic J. curcas plants, the flower number per inflorescence was significantly increased, and most flowers were pistil-predominantly bisexual”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
Most flowers of the transgenic Jatropha curcas plants were pistil-predominantly bisexual.
- Taxon
- Jatropha curcasJatropha curcast0
- Material
- transgenic J. curcas plantse3
- Organ / stage
- flowerse3
- Outcome
- pistil-predominantly bisexuale3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In the transgenic J. curcas plants, the flower number per inflorescence was significantly increased, and most flowers were pistil-predominantly bisexual”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
The male and bisexual flowers of transgenic Jatropha curcas were infertile.
- Taxon
- Jatropha curcasJatropha curcast0
- Material
- transgenic J. curcase4
- Organ / stage
- the male and the bisexual flowerse4
- Outcome
- infertilee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Unfortunately, both the male and the bisexual flowers of transgenic J. curcas were infertile”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
The number and position of floral organs in transgenic flowers were well defined.
- Taxon
- Jatropha curcasJatropha curcast0
- Material
- transgenic flowerse5
- Outcome
- number and position of floral organse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the number and position of floral organs in the transgenic flowers were well defined, which suggested that the determinacy of the floral meristem was not affected”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
The determinacy of the floral meristem was not affected in transgenic Jatropha curcas.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- determinacy of the floral meristeme5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the number and position of floral organs in the transgenic flowers were well defined, which suggested that the determinacy of the floral meristem was not affected”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
Fine-tuning endogenous cytokinins is suggested to increase flower number and the female-to-male ratio in Jatropha curcas.
- Taxon
- Jatropha curcasJatropha curcast0
- Outcome
- fine-tuning the endogenous cytokinins can increase the flower number and the female-to-male ratioe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These results suggest that fine-tuning the endogenous cytokinins can increase the flower number and the female-to-male ratio in J. curcas”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
The levels of six cytokinin species in the inflorescences of transgenic Jatropha curcas were elevated.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- transgenic expression of a cytokinin biosynthetic gene (AtIPT4)e1
- Outcome
- levels of six cytokinin species in the inflorescencese2
- Direction
- elevatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“through transgenic expression of a cytokinin biosynthetic gene (AtIPT4) from Arabidopsis under the control of a J. curcas orthologue of TOMATO MADS BOX GENE 6 (JcTM6) promoter”
e2“the levels of six cytokinin species in the inflorescences were elevated, and flower development was modified without any alterations in vegetative growth”
t0“Perennial Woody Plant Jatropha curcas”
Claim · model-written summary
Flower-specific cytokinin overproduction did not alter vegetative growth in Jatropha curcas.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- transgenic expression of a cytokinin biosynthetic gene (AtIPT4)e1
- Outcome
- vegetative growthe2
- Direction
- without any alterationse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“through transgenic expression of a cytokinin biosynthetic gene (AtIPT4) from Arabidopsis under the control of a J. curcas orthologue of TOMATO MADS BOX GENE 6 (JcTM6) promoter”
e2“the levels of six cytokinin species in the inflorescences were elevated, and flower development was modified without any alterations in vegetative growth”
t0“Perennial Woody Plant Jatropha curcas”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.