Abstract
The biodiesel plant, Jatropha curcas L. was micropropagated using nodal explants on MS supplemented with BAP (1.5 mg/l), Kn (0.5 mg/l) and IAA (0.1 mg/l). Somatic embryos were induced directly from green cotyledon explants on MS fortified with 2 mg/l of BAP. The results of histological studies showed that several layers of meristematic cells participated in the formation of somatic embryos. Rooting was effectively achieved on MS supplemented with IAA at 1.0 mg/l. The subsequent hardening experiment showed that the commercial medium containing a mixture of decomposed coir waste, perlite and organic compost in the ratio of 1:1:1 by volume was most effective, 80% plantlets survived. Key words: Jatropha curcas, Nodal explants, Cotyledons, Somatic Embryos, Multiple shoot formation D.O.I. 10.3329/ptcb.v17i2.3234 Plant Tissue Cult. & Biotech. 17(2): 137-147, 2007 (December)
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Claims 5
Claim · model-written summary
Nodal explants were micropropagated on MS with BAP, Kn and IAA.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Organ / stage
- nodal explantse0
- Treatment
- MS supplemented with BAP (1.5 mg/l), Kn (0.5 mg/l) and IAA (0.1 mg/l)e0
- Outcome
- micropropagatede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“The biodiesel plant, Jatropha curcas L. was micropropagated using nodal explants on MS supplemented with BAP (1.5 mg/l), Kn (0.5 mg/l) and IAA (0.1 mg/l).”
Claim · model-written summary
Green cotyledons formed somatic embryos directly on MS with BAP.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- green cotyledon explantse0
- Treatment
- MS fortified with 2 mg/l of BAPe0
- Outcome
- Somatic embryos were induced directlye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos were induced directly from green cotyledon explants on MS fortified with 2 mg/l of BAP.”
t0“Jatropha curcas L.”
Claim · model-written summary
Several meristematic cell layers participated in somatic embryo formation.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- meristematic cellse0
- Outcome
- formation of somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several layers of meristematic cells participated in the formation of somatic embryos”
t0“Jatropha curcas L.”
Claim · model-written summary
MS with IAA effectively achieved rooting.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS supplemented with IAA at 1.0 mg/le0
- Outcome
- Rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting was effectively achieved on MS supplemented with IAA at 1.0 mg/l.”
t0“Jatropha curcas L.”
Claim · model-written summary
A coir, perlite and compost medium gave the most effective hardening, with 80% survival.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- commercial medium containing a mixture of decomposed coir waste, perlite and organic compost in the ratio of 1:1:1 by volumee0
- Outcome
- plantlets survivede0
- Direction
- most effectivee0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the commercial medium containing a mixture of decomposed coir waste, perlite and organic compost in the ratio of 1:1:1 by volume was most effective, 80% plantlets survived”
t0“Jatropha curcas L.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.