Abstract
Micropropagation protocols depend on several factors, particularly on the types, combinations, and levels of plant growth regulators used in the culture media. The aim of this study was to determine the influence of 6-Benzylaminopurine (BAP) and Naphtalen-3acetic acid (NAA) concentrations on callus induction of Jatropha curcas L. explants. The plant material used was a jatropha hybrid accession developed through intraspecific crosses between Costa Rica and Mexico genetic resources (TREC 49 x TREC 33 accession codes). Callus cultures were initiated from hypocotyl explants isolated from 15-days-old seedlings, on Murashige and Skoog (MS) basal medium supplemented with 0; 2 or 4 mg L -1 BAP and 0; 1 or 2 mg L -1 NAA in different combinations. Better-quality callus formation was obtained in medium supplemented with BAP 2 mg L -1 and NAA 1 mg L -1 . This combination of plant growth regulators resulted in higher amount of white colored and friable calli, which are more likely to differentiate in bud shoots and roots, depending upon the plant growth regulations added to the media. Our results suggest that BAP (2 mg L -1 ) and NAA (1 mg L -1 ) combined can be used for in vitro callogenesis of J. curcas hybrid accession and that it can be adapted to a large-scale in vitro mass production system or to support biotechnological protocols.
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Claims 4
Claim · model-written summary
BAP at 2 mg L -1 with NAA at 1 mg L -1 produced better-quality callus.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Treatment
- BAP 2 mg L -1 and NAA 1 mg L -1e0
- Outcome
- Better-quality callus formatione0
- Direction
- Better-qualitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
t0“Jatropha curcas L.”
e0“Better-quality callus formation was obtained in medium supplemented with BAP 2 mg L -1 and NAA 1 mg L -1 .”
Claim · model-written summary
The BAP and NAA combination produced a higher amount of white, friable calli.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- BAP 2 mg L -1 and NAA 1 mg L -1e1
- Outcome
- white colored and friable callie0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“This combination of plant growth regulators resulted in higher amount of white colored and friable calli”
e1“BAP 2 mg L -1 and NAA 1 mg L -1”
Claim · model-written summary
The authors recommend combined BAP and NAA for in vitro callogenesis of the Jatropha hybrid accession.
- Taxon
- J. curcasJatropha curcase0
- Material
- hybrid accessione0
- Treatment
- BAP (2 mg L -1 ) and NAA (1 mg L -1 ) combinede0
- Outcome
- in vitro callogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP (2 mg L -1 ) and NAA (1 mg L -1 ) combined can be used for in vitro callogenesis of J. curcas hybrid accession”
Claim · model-written summary
The authors suggest adapting the combination to large-scale in vitro mass production or biotechnological protocols.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- large-scale in vitro mass production system or to support biotechnological protocolse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“it can be adapted to a large-scale in vitro mass production system or to support biotechnological protocols.”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.