Abstract
F-box proteins play important roles in plant growth/development and responses to environmental stimuli through targeting substrates into degradation machinery. A rice defense-related F-box protein gene, OsDRF1, was cloned and identified during a course of study aimed at elucidating the molecular basis of induced immunity in rice. OsDRF1 encodes a protein of 328 amino acids, containing a highly conserved F-box domain. Expression of OsDRF1 was induced upon treatment with benzothiadiazole (BTH), a chemical inducer of defense responses in rice. Moreover, in BTH-treated rice seedlings, expression of OsDRF1 was further induced by infection with Magnaporthe grisea, the rice blast fungus, compared with those in water-treated seedlings. OsDRF1 was also upregulated in rice seedlings after treatment with ABA. Overexpression of OsDRF1 in transgenic tobacco resulted in enhanced disease resistance against tomato mosaic virus (ToMV) and Pseudomonas syringae pv. tabaci and strengthened expression of defense-related genes after salicylic acid treatment or ToMV infection. Root elongation of the OsDRF1-overexpressing transgenic seedlings was significantly inhibited by ABA, indicating that overexpression of OsDRF1 resulted in increased ABA sensitivity. The results suggest that OsDRF1 plays a role in disease resistance via upregulating defense-related gene expression and that OsDRF1 may also be involved in the response to ABA.
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Claims 10
Claim · model-written summary
Rice OsDRF1 encodes a 328 amino acid protein with an F-box domain.
- Taxon
- rice
Oryza sativat0 - Gene
- OsDRF1e0
- Outcome
- protein of 328 amino acidse0
- Value
- 328 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDRF1 encodes a protein of 328 amino acids, containing a highly conserved F-box domain.”
t0“Overexpression of a rice defense‐related F‐box protein gene OsDRF1 in tobacco improves disease resistance through potentiation of defense gene expression †”
Claim · model-written summary
BTH induced OsDRF1 expression in rice.
- Taxon
- riceOryza sativae0
- Treatment
- benzothiadiazole (BTH)e0
- Gene
- OsDRF1e0
- Outcome
- Expression of OsDRF1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsDRF1 was induced upon treatment with benzothiadiazole (BTH), a chemical inducer of defense responses in rice.”
Claim · model-written summary
Magnaporthe grisea infection further induced OsDRF1 in BTH-treated rice seedlings compared with water-treated seedlings.
- Taxon
- riceOryza sativae0
- Treatment
- infection with Magnaporthe griseae0
- Interactor
- Magnaporthe grisea
Pyricularia griseae0pathogen - Gene
- OsDRF1e0
- Outcome
- expression of OsDRF1e0
- Direction
- further inducede0
- Comparator
- water-treated seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in BTH-treated rice seedlings, expression of OsDRF1 was further induced by infection with Magnaporthe grisea, the rice blast fungus, compared with those in water-treated seedlings.”
Claim · model-written summary
ABA upregulated OsDRF1 in rice seedlings.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDRF1 was also upregulated in rice seedlings after treatment with ABA.”
Claim · model-written summary
OsDRF1 overexpression enhanced tobacco resistance to tomato mosaic virus.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of OsDRF1e0
- Interactor
- tomato mosaic virus (ToMV)
Tomato mosaic viruse0pathogen - Gene
- OsDRF1e0
- Outcome
- disease resistance against tomato mosaic virus (ToMV)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsDRF1 in transgenic tobacco resulted in enhanced disease resistance against tomato mosaic virus (ToMV) and Pseudomonas syringae pv. tabaci”
Claim · model-written summary
OsDRF1 overexpression enhanced tobacco resistance to Pseudomonas syringae pv. tabaci.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of OsDRF1e0
- Interactor
- Pseudomonas syringae pv. tabaciPseudomonas amygdali pv. tabacie0pathogen
- Gene
- OsDRF1e0
- Outcome
- disease resistance against tomato mosaic virus (ToMV) and Pseudomonas syringae pv. tabacie0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsDRF1 in transgenic tobacco resulted in enhanced disease resistance against tomato mosaic virus (ToMV) and Pseudomonas syringae pv. tabaci”
Claim · model-written summary
OsDRF1 overexpression strengthened defense gene expression after salicylic acid treatment or ToMV infection in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of OsDRF1e0
- Interactor
- ToMVe0pathogen
- Gene
- OsDRF1e0
- Outcome
- expression of defense-related genese0
- Direction
- strengthenede0
- Qualifier
- after salicylic acid treatment or ToMV infectione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsDRF1 in transgenic tobacco resulted in enhanced disease resistance against tomato mosaic virus (ToMV) and Pseudomonas syringae pv. tabaci and strengthened expression of defense-related genes after salicylic acid treatment or ToMV infection.”
Claim · model-written summary
ABA significantly inhibited root elongation in OsDRF1-overexpressing seedlings.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- ABAe0
- Gene
- OsDRF1e0
- Outcome
- Root elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root elongation of the OsDRF1-overexpressing transgenic seedlings was significantly inhibited by ABA”
t0“Overexpression of a rice defense‐related F‐box protein gene OsDRF1 in tobacco improves disease resistance through potentiation of defense gene expression †”
Claim · model-written summary
The authors suggest OsDRF1 acts in disease resistance through defense gene upregulation.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- OsDRF1e0
- Outcome
- disease resistancee0
- Qualifier
- upregulating defense-related gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDRF1 plays a role in disease resistance via upregulating defense-related gene expression”
t0“Overexpression of a rice defense‐related F‐box protein gene OsDRF1 in tobacco improves disease resistance through potentiation of defense gene expression †”
Claim · model-written summary
The authors suggest OsDRF1 participates in ABA response.
- Taxon
- riceOryza sativat0
- Gene
- OsDRF1e0
- Outcome
- response to ABAe0
- Qualifier
- may also be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDRF1 may also be involved in the response to ABA”
t0“Overexpression of a rice defense‐related F‐box protein gene OsDRF1 in tobacco improves disease resistance through potentiation of defense gene expression †”
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See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.