Paper · 2013 · Expedition

The effect of different wavelengths on the germination time of Arabidopsis thaliana wild type and mutant type seeds

Andy Byun; Michael W.H. Mao; Randeep Sidhu

6
claims
13
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

The main objective of this experiment was to investigate how different light wavelengths affect the seed germination of Arabidopsis thaliana mutant, cer10, and wild types. In this experiment, we covered trays of petri dishes that had wild-type and mutant seeds with different coloured acetate sheets: red, blue, green, and clear as control. The seeds were grown in a 17 degrees Celsius incubator, and we observed and recorded the growth of Arabidopsis thaliana using light microscopes for approximately one week. After 72 hours, it was observed that the mean number of germinated seeds was the highest (5.75 ± 2.387 for mutant and 5.25 ± 0.796 for wild type) for natural light compared to other colours even though the differences were not statistically significant. After 96 hours, red light had a significantly larger number of germinated seeds (9.5 ± 0.919) compared with other wavelengths of light for both mutant and wild types; red light correlated to faster seed germination. The difference was statistically significant between red and blue light, even though the trend was still present in the comparison of the red light with the other two wavelengths. We reject our null hypothesis; red wavelengths of light will slow or have no effect on the germination of Arabidopsis thaliana. Additionally, we tested another set of hypotheses regarding the difference in the number of germinated mutant cer10 and wild type seeds. According to the results, we fail to reject our null hypothesis; mutant type seeds germinate faster.

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Claims 6

Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

After 72 hours natural light gave the highest mutant germination mean, 5.75 ± 2.387, without significant differences.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Material
mutante0
Treatment
natural lighte0
Outcome
mean number of germinated seedse0
Direction
higheste0
Value
5.75 ± 2.387e0
Comparator
other colourse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 72 hours, it was observed that the mean number of germinated seeds was the highest (5.75 ± 2.387 for mutant and 5.25 ± 0.796 for wild type) for natural light compared to other colours even though the differences were not statistically significant”

✓ located verbatim in the source · abstract, characters 513–765 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑
Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

After 72 hours natural light gave the highest wild-type germination mean, 5.25 ± 0.796, without significant differences.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Material
wild typee0
Treatment
natural lighte0
Outcome
mean number of germinated seedse0
Direction
higheste0
Value
5.25 ± 0.796e0
Comparator
other colourse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 72 hours, it was observed that the mean number of germinated seeds was the highest (5.75 ± 2.387 for mutant and 5.25 ± 0.796 for wild type) for natural light compared to other colours even though the differences were not statistically significant”

✓ located verbatim in the source · abstract, characters 513–765 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

After 96 hours red light produced 9.5 ± 0.919 germinated seeds, significantly more than other wavelengths.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Treatment
red lighte0
Outcome
number of germinated seedse0
Direction
largere0
Value
9.5 ± 0.919e0
Comparator
other wavelengths of lighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“After 96 hours, red light had a significantly larger number of germinated seeds (9.5 ± 0.919) compared with other wavelengths of light for both mutant and wild types”

✓ located verbatim in the source · abstract, characters 767–932 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Red light was associated with faster germination.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Treatment
red lighte0
Outcome
seed germinatione0
Direction
fastere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“red light correlated to faster seed germination”

✓ located verbatim in the source · abstract, characters 934–981 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The red-blue germination difference was statistically significant.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Treatment
rede0
Outcome
number of germinated seedse1
Comparator
blue lighte0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The difference was statistically significant between red and blue light”

✓ located verbatim in the source · abstract, characters 983–1054 · show in text ↑
e1

“significantly larger number of germinated seeds”

✓ located verbatim in the source · abstract, characters 799–846 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑
Observationobservedoriginal resultnot significant (as stated)measured

Claim · model-written summary

The authors reported failing to reject the mutant versus wild-type null hypothesis.

Taxon
Arabidopsis thalianaArabidopsis thalianat0
Material
mutant typee0
Outcome
seeds germinate fastere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“we fail to reject our null hypothesis; mutant type seeds germinate faster”

✓ located verbatim in the source · abstract, characters 1454–1527 · show in text ↑
t0

“Arabidopsis thaliana”

✓ located verbatim in the source · title, characters 63–83 · show in text ↑

light · growth · Arabidopsis thaliana (thale cress)Results differ

44 papers: 30 positive, 14 negative or no effect

29 papers in the same direction as this paper · 14 in the opposite direction

  1. positive
  2. negative
  3. negative

40 papers more in this group

See all 44 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.