Abstract
Summary: In this paper, the efficiency of three techniques (two-step freezing, vitrification; encapsulation-dehydion) for freezing in vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. Cold-hardening mother plants for 3 weeks at 5OC improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. Increasing the time during which mother-plants were maintained on standard' medium without subculture before sampling of apices decreased the water content of shoot tips from about 8548% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulationdehydration technique. Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apices, thus simplifying the protocol. This .simplified encapsulation-dehydration protocol was successfully applied. to 11 out of the 12 cultivars tested. a .?. 'vp
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Claims 6
Claim · model-written summary
Cold hardening donor apple plants improved shoot tip regrowth across freezing techniques.
- Taxon
- apple
Malus domesticat0 - Treatment
- Cold-hardening mother plants for 3 weeks at 5OCe0
- Outcome
- regrowth rate and pattern of shoot tipse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold-hardening mother plants for 3 weeks at 5OC improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed.”
t0“apple”
Claim · model-written summary
Longer periods without subculture reduced apple shoot tip water content.
- Taxon
- appleMalus domesticat0
- Treatment
- Increasing the time during which mother-plants were maintained on standard' medium without subculturee0
- Outcome
- water content of shoot tipse0
- Direction
- decreasede0
- Value
- 63-66%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the time during which mother-plants were maintained on standard' medium without subculture before sampling of apices decreased the water content of shoot tips from about 8548% to 63-66%”
t0“apple”
Claim · model-written summary
Longer periods without subculture increased apple shoot tip regrowth.
- Taxon
- appleMalus domesticat0
- Treatment
- Increasing the time during which mother-plants were maintained on standard' medium without subculturee0
- Outcome
- regrowth rate and patterne0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the time during which mother-plants were maintained on standard' medium without subculture before sampling of apices decreased the water content of shoot tips from about 8548% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed.”
t0“apple”
Claim · model-written summary
Encapsulation dehydration produced up to 86% apple shoot tip regrowth without callusing.
- Taxon
- appleMalus domesticat0
- Treatment
- encapsulationdehydration techniquee0
- Outcome
- regrowth after cryopreservatione0
- Value
- 86%e0
- Comparator
- best resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulationdehydration technique.”
t0“apple”
Claim · model-written summary
Unsubcultured donor plants allowed a shorter sucrose pregrowth treatment.
- Taxon
- appleMalus domesticat0
- Outcome
- duration of the sucrose pregrowth treatmente0
- Value
- 26 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apices”
t0“apple”
Claim · model-written summary
The simplified protocol worked for 11 of 12 tested apple cultivars.
- Taxon
- appleMalus domesticat0
- Outcome
- successfully appliede0
- Value
- 11 out of the 12 cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This .simplified encapsulation-dehydration protocol was successfully applied. to 11 out of the 12 cultivars tested.”
t0“apple”
The same questions in other papers
cold · growth · Malus domestica (apple)Positive and negative results
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- no effect
- negative
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33 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.