Abstract
The Fusarium mycotoxin deoxynivalenol (DON) can cause cell death in wheat (Triticum aestivum), but can also reduce the level of cell death caused by heat shock in Arabidopsis (Arabidopsis thaliana) cell cultures. We show that 10 μg mL(-1) DON does not cause cell death in Arabidopsis cell cultures, and its ability to retard heat-induced cell death is light dependent. Under dark conditions, it actually promoted heat-induced cell death. Wheat cultivars differ in their ability to resist this toxin, and we investigated if the ability of wheat to mount defense responses was light dependent. We found no evidence that light affected the transcription of defense genes in DON-treated roots of seedlings of two wheat cultivars, namely cultivar CM82036 that is resistant to DON-induced bleaching of spikelet tissue and cultivar Remus that is not. However, DON treatment of roots led to genotype-dependent and light-enhanced defense transcript accumulation in coleoptiles. Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene (previously associated with Fusarium resistance), non-expressor of pathogenesis-related genes-1 (NPR1) and a class III plant peroxidase (POX) were DON-upregulated in coleoptiles of wheat cultivar CM82036 but not of cultivar Remus, and DON-upregulation of these transcripts in cultivar CM82036 was light enhanced. Light and genotype-dependent differences in the DON/DON derivative content of coleoptiles were also observed. These results, coupled with previous findings regarding the effect of DON on plants, show that light either directly or indirectly influences the plant defense responses to DON.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
DON at 10 μg mL(-1) does not cause cell death in Arabidopsis cell cultures.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- cell culturese0
- Treatment
- 10 μg mL(-1) DONe0
- Outcome
- cell deathe0
- Direction
- does not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 μg mL(-1) DON does not cause cell death in Arabidopsis cell cultures”
Claim · model-written summary
DON's ability to retard heat-induced cell death in Arabidopsis cell cultures depends on light.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- lighte0
- Outcome
- heat-induced cell deathe0
- Qualifier
- light dependente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that 10 μg mL(-1) DON does not cause cell death in Arabidopsis cell cultures, and its ability to retard heat-induced cell death is light dependent.”
Claim · model-written summary
Under darkness, DON promotes heat-induced cell death in Arabidopsis cell cultures.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- DONe1
- Outcome
- heat-induced cell deathe0
- Direction
- promotede0
- Qualifier
- Under dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under dark conditions, it actually promoted heat-induced cell death.”
e1“10 μg mL(-1) DON does not cause cell death in Arabidopsis cell cultures”
Claim · model-written summary
No evidence was found that light affects defense-gene transcription in DON-treated roots of CM82036 seedlings.
- Taxon
- wheat
Triticum aestivume0 - Material
- CM82036e0
- Organ / stage
- roots of seedlingse0
- Treatment
- lighte0
- Outcome
- transcription of defense genese0
- Direction
- no evidence that light affectede0
- Qualifier
- DON-treatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found no evidence that light affected the transcription of defense genes in DON-treated roots of seedlings of two wheat cultivars, namely cultivar CM82036 that is resistant to DON-induced bleaching of spikelet tissue and cultivar Remus that is not.”
Claim · model-written summary
No evidence was found that light affects defense-gene transcription in DON-treated roots of Remus seedlings.
- Taxon
- wheatTriticum aestivume0
- Material
- Remuse0
- Organ / stage
- roots of seedlingse0
- Treatment
- lighte0
- Outcome
- transcription of defense genese0
- Direction
- no evidence that light affectede0
- Qualifier
- DON-treatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We found no evidence that light affected the transcription of defense genes in DON-treated roots of seedlings of two wheat cultivars, namely cultivar CM82036 that is resistant to DON-induced bleaching of spikelet tissue and cultivar Remus that is not.”
Claim · model-written summary
Root DON treatment produces genotype-dependent, light-enhanced defense transcript accumulation in wheat coleoptiles.
- Taxon
- WheatTriticum aestivume1
- Organ / stage
- coleoptilese0
- Treatment
- DON treatment of rootse0
- Outcome
- defense transcript accumulatione0
- Direction
- light-enhancede0
- Qualifier
- genotype-dependente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, DON treatment of roots led to genotype-dependent and light-enhanced defense transcript accumulation in coleoptiles.”
e1“Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene”
Claim · model-written summary
DON upregulates PAL, NPR1 and POX transcripts in CM82036 coleoptiles.
- Taxon
- wheatTriticum aestivume0
- Material
- CM82036e0
- Organ / stage
- coleoptilese0
- Treatment
- DONe0
- Outcome
- transcriptse0
- Direction
- DON-upregulatede0
- Qualifier
- a phenylalanine ammonia lyase (PAL) gene (previously associated with Fusarium resistance), non-expressor of pathogenesis-related genes-1 (NPR1) and a class III plant peroxidase (POX)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene (previously associated with Fusarium resistance), non-expressor of pathogenesis-related genes-1 (NPR1) and a class III plant peroxidase (POX) were DON-upregulated in coleoptiles of wheat cultivar CM82036 but not of cultivar Remus”
Claim · model-written summary
DON does not upregulate PAL, NPR1 and POX transcripts in Remus coleoptiles.
- Taxon
- wheatTriticum aestivume0
- Material
- Remuse0
- Organ / stage
- coleoptilese0
- Treatment
- DONe0
- Outcome
- transcriptse0
- Direction
- no_changeinferrede0
- Qualifier
- but not of cultivar Remuse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene (previously associated with Fusarium resistance), non-expressor of pathogenesis-related genes-1 (NPR1) and a class III plant peroxidase (POX) were DON-upregulated in coleoptiles of wheat cultivar CM82036 but not of cultivar Remus”
Claim · model-written summary
Light enhances DON upregulation of defense transcripts in CM82036 coleoptiles.
- Taxon
- WheatTriticum aestivume1
- Material
- CM82036e0
- Organ / stage
- coleoptilese1
- Treatment
- lighte0
- Outcome
- DON-upregulation of these transcriptse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DON-upregulation of these transcripts in cultivar CM82036 was light enhanced.”
e1“Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene (previously associated with Fusarium resistance), non-expressor of pathogenesis-related genes-1 (NPR1) and a class III plant peroxidase (POX) were DON-upregulated in coleoptiles”
Claim · model-written summary
Wheat coleoptile DON and DON derivative content differs with light and genotype.
- Taxon
- WheatTriticum aestivume1
- Organ / stage
- coleoptilese0
- Treatment
- Lighte0
- Outcome
- DON/DON derivative contente0
- Qualifier
- genotype-dependent differencese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light and genotype-dependent differences in the DON/DON derivative content of coleoptiles were also observed.”
e1“Wheat transcripts encoding a phenylalanine ammonia lyase (PAL) gene”
Claim · model-written summary
The authors infer that light directly or indirectly influences plant defense responses to DON.
Verbatim
e0“light either directly or indirectly influences the plant defense responses to DON.”
The same questions in other papers
deoxynivalenol · cytology · ArabidopsisResults agree
1 paper in the same direction as this paper
- negative
deoxynivalenol · molecular: expression · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- positive
light · molecular: expression · Triticum aestivum (wheat)Positive and negative results
12 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- negative
- negative
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.