Abstract
The apoplast of plant cells, which carries out multiple functions in plant metabolism and signaling, is not only a barrier but also the linker between the environment and the protoplast. To investigate the role of apoplastic proteins in the salt stress response, 10-d-old rice (Oryza sativa) plants were treated with 200 mM NaCl for 1, 3, or 6 h, and the soluble apoplast proteins were extracted for differential analysis compared with untreated controls using two-dimensional electrophoresis. Ten protein spots that increased or decreased significantly in abundance were identified by mass spectrometry. These proteins included some well-known biotic and abiotic stress-related proteins. Among them, an apoplastic protein, with extracellular domain-like cysteine-rich motifs (DUF26), O. sativa root meander curling (OsRMC), has shown drastically increased abundance in response to salt stress during the initial phase. OsRMC RNA interference transgenic rice has been generated to assess the function of OsRMC in the salt stress response. The results show that knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition, and improved salt stress tolerance to NaCl than in untransgenic plants. These results indicate that plant apoplastic proteins may have important roles in the plant salt stress response.
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Claims 5
Claim · model-written summary
Ten apoplast protein spots changed significantly in abundance after salt treatment.
- Taxon
- rice
Oryza sativat0 - Treatment
- 200 mM NaCle1
- Outcome
- protein spotse0
- Direction
- increased or decreasede0
- Value
- Tene0
- Qualifier
- significantly in abundancee0
- Comparator
- untreated controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ten protein spots that increased or decreased significantly in abundance were identified by mass spectrometry.”
t0“Rice”
e1“treated with 200 mM NaCl for 1, 3, or 6 h, and the soluble apoplast proteins were extracted for differential analysis compared with untreated controls”
Claim · model-written summary
OsRMC apoplastic protein abundance increased drastically during initial salt stress.
- Taxon
- riceOryza sativat0
- Treatment
- salt stresse0
- Gene
- O. sativa root meander curling (OsRMC)e0
- Outcome
- abundancee0
- Direction
- increasede0
- Qualifier
- during the initial phasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an apoplastic protein, with extracellular domain-like cysteine-rich motifs (DUF26), O. sativa root meander curling (OsRMC), has shown drastically increased abundance in response to salt stress during the initial phase.”
t0“Rice”
Claim · model-written summary
OsRMC knockdown produced insensitive seed germination under salt stress.
- Taxon
- riceOryza sativae0
- Treatment
- knocking down the expression level of OsRMCe0
- Gene
- OsRMCe0
- Outcome
- insensitive seed germinatione0
- Qualifier
- NaCle0
- Comparator
- untransgenic plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition, and improved salt stress tolerance to NaCl than in untransgenic plants.”
Claim · model-written summary
OsRMC knockdown enhanced growth inhibition relative to untransgenic plants under NaCl.
- Taxon
- riceOryza sativae0
- Treatment
- knocking down the expression level of OsRMCe0
- Gene
- OsRMCe0
- Outcome
- growth inhibitione0
- Direction
- enhancede0
- Qualifier
- NaCle0
- Comparator
- untransgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition, and improved salt stress tolerance to NaCl than in untransgenic plants.”
Claim · model-written summary
OsRMC knockdown improved salt stress tolerance relative to untransgenic plants.
- Taxon
- riceOryza sativae0
- Treatment
- knocking down the expression level of OsRMCe0
- Gene
- OsRMCe0
- Outcome
- salt stress tolerance to NaCle0
- Direction
- improvede0
- Comparator
- untransgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knocking down the expression level of OsRMC in transgenic rice led to insensitive seed germination, enhanced growth inhibition, and improved salt stress tolerance to NaCl than in untransgenic plants.”
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