Abstract
Pseudomonas syringae pv. tomato DC3000 causes bacterial speck disease in tomato, and it elicits the hypersensitive response (HR) in non-host plants such as Nicotiana tabacum and Nicotiana benthamiana. The compatible and incompatible interactions of DC3000 with tomato and Nicotiana spp., respectively, result in plant cell death, but the HR cell death occurs more rapidly and is associated with effective plant defense. Both interactions require the Hrp (HR and pathogenicity) type III secretion system (TTSS), which injects Hop (Hrp outer protein) effectors into plant cells. Here, we demonstrate that HopPtoN is translocated into tomato cells via the Hrp TTSS. A hopPtoN mutant produced eightfold more necrotic 'speck' lesions on tomato leaves than did DC3000, but the mutant and the wild-type strain grew to the same level in infected leaves. In non-host N. tabacum leaves, the hopPtoN mutant produced more cell death, whereas a DC3000 strain overexpressing HopPtoN produced less cell death and associated electrolyte leakage in comparison with wild-type DC3000. Transient expression of HopPtoN via infection with a PVX viral vector enabled tomato and N. benthamiana plants to tolerate, with reduced disease lesions, challenge infections with DC3000 and P. syringae pv. tabaci 11528, respectively. HopPtoN showed cysteine protease activity in vitro, and hopPtoN mutants altered in the predicted cysteine protease catalytic triad (C172S, H283A and D299A) lost HR suppression activity. These observations reveal that HopPtoN is a TTSS effector that can suppress plant cell death events in both compatible and incompatible interactions.
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Claims 9
Claim · model-written summary
HopPtoN was translocated into tomato cells through Hrp TTSS.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- Pseudomonas syringae
Pseudomonas syringaet0pathogen - Gene
- HopPtoNe0
- Outcome
- translocated into tomato cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HopPtoN is translocated into tomato cells via the Hrp TTSS”
t0“Pseudomonas syringae”
Claim · model-written summary
The hopPtoN mutant caused eightfold more necrotic lesions than DC3000.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- hopPtoN mutante0
- Interactor
- DC3000e0pathogen
- Gene
- hopPtoNe0
- Outcome
- necrotic 'speck' lesionse0
- Direction
- moree0
- Value
- eightfolde0
- Comparator
- DC3000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A hopPtoN mutant produced eightfold more necrotic 'speck' lesions on tomato leaves than did DC3000”
Claim · model-written summary
The mutant and wild type reached the same level in infected leaves.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- DC3000e0pathogen
- Outcome
- grew to the same level in infected leavese0
- Direction
- same levele0
- Comparator
- wild-type straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A hopPtoN mutant produced eightfold more necrotic 'speck' lesions on tomato leaves than did DC3000, but the mutant and the wild-type strain grew to the same level in infected leaves.”
Claim · model-written summary
The hopPtoN mutant caused more cell death in N. tabacum leaves.
- Taxon
- N. tabacum
Nicotiana tabacume0 - Treatment
- hopPtoN mutante0
- Interactor
- Pseudomonas syringaePseudomonas syringaet0pathogen
- Outcome
- cell deathe0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In non-host N. tabacum leaves, the hopPtoN mutant produced more cell death”
t0“Pseudomonas syringae”
Claim · model-written summary
HopPtoN overexpression reduced cell death and electrolyte leakage in N. tabacum.
- Taxon
- N. tabacumNicotiana tabacume0
- Treatment
- DC3000 strain overexpressing HopPtoNe0
- Interactor
- DC3000e0pathogen
- Outcome
- cell death and associated electrolyte leakagee0
- Direction
- lesse0
- Comparator
- wild-type DC3000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In non-host N. tabacum leaves, the hopPtoN mutant produced more cell death, whereas a DC3000 strain overexpressing HopPtoN produced less cell death and associated electrolyte leakage in comparison with wild-type DC3000.”
t0“Nicotiana tabacum”
Claim · model-written summary
PVX-mediated HopPtoN expression reduced tomato lesions after DC3000 challenge.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Transient expression of HopPtoN via infection with a PVX viral vectore0
- Interactor
- DC3000e0pathogen
- Outcome
- disease lesionse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of HopPtoN via infection with a PVX viral vector enabled tomato and N. benthamiana plants to tolerate, with reduced disease lesions, challenge infections with DC3000 and P. syringae pv. tabaci 11528, respectively.”
Claim · model-written summary
PVX-mediated HopPtoN expression reduced N. benthamiana lesions after P. syringae pv. tabaci challenge.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- Transient expression of HopPtoN via infection with a PVX viral vectore0
- Interactor
- P. syringae pv. tabaci 11528Pseudomonas amygdali pv. tabacie0pathogen
- Outcome
- disease lesionse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of HopPtoN via infection with a PVX viral vector enabled tomato and N. benthamiana plants to tolerate, with reduced disease lesions, challenge infections with DC3000 and P. syringae pv. tabaci 11528, respectively.”
Claim · model-written summary
HopPtoN had cysteine protease activity in vitro.
- Interactor
- Pseudomonas syringaePseudomonas syringaet0pathogen
- Gene
- HopPtoNe0
- Outcome
- cysteine protease activitye0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HopPtoN showed cysteine protease activity in vitro”
t0“Pseudomonas syringae”
Claim · model-written summary
Catalytic-triad mutants lost HR suppression.
- Taxon
- Nicotiana tabacumNicotiana tabacume1
- Treatment
- hopPtoN mutants altered in the predicted cysteine protease catalytic triade0
- Interactor
- Pseudomonas syringaePseudomonas syringaet0pathogen
- Gene
- hopPtoNe0
- Outcome
- HR suppression activitye0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hopPtoN mutants altered in the predicted cysteine protease catalytic triad (C172S, H283A and D299A) lost HR suppression activity”
t0“Pseudomonas syringae”
e1“Nicotiana tabacum”
The same questions in other papers
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- negative
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transgenic · disease · Solanum lycopersicum (tomato)Positive and negative results
15 papers in the same direction as this paper · 10 in the opposite direction · 1 reporting no difference from an alternative
- negative
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See all 27 papers →infection · disease · Solanum lycopersicum (tomato)Positive and negative results
28 papers in the same direction as this paper · 27 in the opposite direction
- negative
- no effect
- negative
52 papers more in this group
See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.