Abstract
Several cold-regulated (COR) polypeptides, which have little or no amino acid sequence identity with known proteins, are synthesized during cold acclimation of Arabidopsis thaliana. However, the function of the polypeptides has yet to be elucidated. The objective of this study was to determine if COR6.6 and COR15am influence the incidence of either freeze-induced fusion or freeze-induced leakage of small unilamellar vesicles (SUVs) composed of either a single species of phosphatidylcholine (either 1-palmitoyl-2-oleoyl-,dioleoyl-, or dilinoleoylphosphatidylcholine), a mixture of dioleoylphosphatidylcholine, dioleoylphosphatidylethanolamine, and free sterols (1:1:1, mol:mol), or the total lipid extract of the plasma membrane of either nonacclimated or cold-acclimated rye leaves. When the SUVs were suspended in a dilute tris(hydroxymethyl)-aminomethane/2-(N-morpholino) ethanesulfonic acid buffer, both COR6.6 and COR15am invariably decreased the incidence of freeze-induced fusion regardless of the lipid composition. However, if the SUVs were suspended in a dilute solution of either sucrose or NaCl, the COR polypeptides had little or no effect on the incidence of freeze-induced fusion. Moreover, the COR polypeptides did not decrease the incidence of freeze-induced leakage--regardless of whether the SUVs were suspended in either the dilute buffer alone or with added sucrose or NaCl. In fact, with SUVs composed of a single species of phosphatidylcholine suspended in the dilute buffer, the COR polypeptides resulted in an anomalous increase in freeze-induced leakage. When considered collectively, these results suggest that neither COR6.6 nor COR15am has a direct cryoprotective effect on SUVs frozen in vitro.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
COR6.6 decreased freeze-induced fusion of SUVs in dilute buffer regardless of lipid composition.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- COR6.6e1
- Gene
- COR6.6e1
- Outcome
- incidence of freeze-induced fusione1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“When the SUVs were suspended in a dilute tris(hydroxymethyl)-aminomethane/2-(N-morpholino) ethanesulfonic acid buffer, both COR6.6 and COR15am invariably decreased the incidence of freeze-induced fusion regardless of the lipid composition.”
Claim · model-written summary
COR15am decreased freeze-induced fusion of SUVs in dilute buffer regardless of lipid composition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- COR15ame1
- Gene
- COR15ame1
- Outcome
- incidence of freeze-induced fusione1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“When the SUVs were suspended in a dilute tris(hydroxymethyl)-aminomethane/2-(N-morpholino) ethanesulfonic acid buffer, both COR6.6 and COR15am invariably decreased the incidence of freeze-induced fusion regardless of the lipid composition.”
Claim · model-written summary
In dilute sucrose or NaCl, COR polypeptides had little or no effect on freeze-induced fusion.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- COR polypeptidese1
- Outcome
- incidence of freeze-induced fusione1
- Direction
- little or no effecte1
- Qualifier
- a dilute solution of either sucrose or NaCle1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“if the SUVs were suspended in a dilute solution of either sucrose or NaCl, the COR polypeptides had little or no effect on the incidence of freeze-induced fusion.”
Claim · model-written summary
COR polypeptides did not decrease freeze-induced leakage in the tested suspensions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- COR polypeptidese1
- Outcome
- incidence of freeze-induced leakagee1
- Direction
- did not decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“the COR polypeptides did not decrease the incidence of freeze-induced leakage--regardless of whether the SUVs were suspended in either the dilute buffer alone or with added sucrose or NaCl.”
Claim · model-written summary
COR polypeptides anomalously increased freeze-induced leakage for single-phosphatidylcholine SUVs in dilute buffer.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- COR polypeptidese1
- Outcome
- freeze-induced leakagee1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“with SUVs composed of a single species of phosphatidylcholine suspended in the dilute buffer, the COR polypeptides resulted in an anomalous increase in freeze-induced leakage.”
Claim · model-written summary
The authors conclude that neither COR6.6 nor COR15am directly cryoprotects frozen SUVs in vitro.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- direct cryoprotective effect on SUVs frozen in vitroe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold acclimation of Arabidopsis thaliana”
e1“these results suggest that neither COR6.6 nor COR15am has a direct cryoprotective effect on SUVs frozen in vitro.”
The same questions in other papers
cold · disease · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.