Abstract
Abstract In plants, acid invertases are known to be the key enzymes cleaving sucrose into reducing sugars (RS) (glucose and fructose). To improve the quality of potato ( Solanum tuberosum L.) chips, which is largely influenced by RS accumulation in tubers stored at low temperature, a part of acid invertase cDNA with hairpin RNA (hpRNA) structure was transformed into potato cv. N2. Detection of polymerase chain reaction (PCR) amplification and Northern blotting suggested that the RNA interference (RNAi) vector was successfully transformed into cv. N2. The analysis of acid invertase activity in the plantlets and microtubers of RNAi transgenic lines indicated that the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1) with a maximal decrease of 78% (line Ni-4), and the highest decrease of activity in microtubers of 68%. Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity and a potential strategy for post-transcriptional gene silencing (PTGS) in modulation of cold-sweetening in potato.
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Claims 5
Claim · model-written summary
The RNA interference vector was successfully transformed into potato cv. N2.
- Taxon
- potato
Solanum tuberosumt0 - Material
- cv. N2e0
- Outcome
- RNA interference (RNAi) vector was successfully transformede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the RNA interference (RNAi) vector was successfully transformed into cv. N2”
t0“potato (Solanum tuberosumL.) tubers”
Claim · model-written summary
RNAi significantly repressed acid invertase expression in plantlets by an average 69.8%, except line Ni-1.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- plantletse0
- Treatment
- RNAie0
- Outcome
- expression of the acid invertasee0
- Direction
- repressede0
- Value
- 69.8%e0
- Qualifier
- with the exception of line Ni-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets by an average 69.8% (with the exception of line Ni-1)”
t0“potato (Solanum tuberosumL.) tubers”
Claim · model-written summary
Line Ni-4 showed a maximal 78% decrease in acid invertase expression in plantlets.
- Taxon
- potatoSolanum tuberosumt0
- Material
- line Ni-4e0
- Organ / stage
- plantletse1
- Treatment
- RNAie1
- Outcome
- expression of the acid invertasee1
- Direction
- decreasee0
- Value
- 78%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a maximal decrease of 78% (line Ni-4)”
e1“the expression of the acid invertase was significantly repressed by the activity of RNAi of plantlets”
t0“potato (Solanum tuberosumL.) tubers”
Claim · model-written summary
RNAi lines had a highest decrease of 68% in microtuber acid invertase activity.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- microtuberse0
- Treatment
- RNAie1
- Outcome
- activity in microtuberse0
- Direction
- decreasee0
- Value
- 68%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest decrease of activity in microtubers of 68%”
e1“the activity of RNAi”
t0“potato (Solanum tuberosumL.) tubers”
Claim · model-written summary
Compared with antisense inv plants, RNAi produced distinct downregulation of endogenous acid invertase activity.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- RNAie0
- Outcome
- endogenous acid invertase activitye0
- Direction
- downregulatione0
- Comparator
- well-inhibited antisense inv transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with that of well-inhibited antisense inv transgenic plants, the comparative downregulation of RNAi suggests a distinct alteration of endogenous acid invertase activity”
t0“potato (Solanum tuberosumL.) tubers”
The same questions in other papers
loss of function · molecular: expression · Solanum tuberosum (potato)Positive and negative results
5 papers in the same direction as this paper · 11 in the opposite direction
- negative
- positive
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.