Abstract
Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to minimize sprouting and losses due to disease. However, cold temperatures strongly induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulation. This process, referred to as "cold-induced sweetening," is a major postharvest problem for the potato industry. We discovered that the cold-induced expression of VInv is controlled by a 200 bp enhancer, VInvIn2En, located in its second intron. We identified several DNA motifs in VInvIn2En that bind transcription factors involved in the plant cold stress response. Mutation of these DNA motifs abolished VInvIn2En function as a transcriptional enhancer. We developed VInvIn2En deletion lines in both diploid and tetraploid potato using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated gene editing. VInv transcription in cold-stored tubers was significantly reduced in the deletion lines. Interestingly, the VInvIn2En sequence is highly conserved among distantly related Solanum species, including tomato (Solanum lycopersicum) and other non-tuber-bearing species. We conclude that the VInv gene and the VInvIn2En enhancer have adopted distinct roles in the cold stress response in tubers of tuber-bearing Solanum species.
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Claims 6
Claim · model-written summary
A 200 bp intronic enhancer VInvIn2En controls cold-induced VInv expression.
- Taxon
- potato
Solanum tuberosumt0 - Organ / stage
- second introne0
- Gene
- VInvIn2Ene0
- Outcome
- cold-induced expression of VInve0
- Value
- 200 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We discovered that the cold-induced expression of VInv is controlled by a 200 bp enhancer, VInvIn2En, located in its second intron.”
t0“potato”
Claim · model-written summary
DNA motifs in VInvIn2En bind transcription factors involved in cold stress response.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- VInvIn2Ene0
- Outcome
- DNA motifs in VInvIn2En that bind transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified several DNA motifs in VInvIn2En that bind transcription factors involved in the plant cold stress response.”
t0“potato”
Claim · model-written summary
Mutation of the DNA motifs abolished VInvIn2En enhancer function.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Mutation of these DNA motifse0
- Gene
- VInvIn2Ene0
- Outcome
- VInvIn2En function as a transcriptional enhancere0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of these DNA motifs abolished VInvIn2En function as a transcriptional enhancer.”
e1“We identified several DNA motifs in VInvIn2En”
t0“potato”
Claim · model-written summary
VInv transcription was significantly reduced in cold-stored tubers after VInvIn2En deletion.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- cold-stored tuberse0
- Treatment
- VInvIn2En deletion linese1
- Outcome
- VInv transcriptione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VInv transcription in cold-stored tubers was significantly reduced in the deletion lines.”
e1“We developed VInvIn2En deletion lines in both diploid and tetraploid potato”
t0“potato”
Claim · model-written summary
VInvIn2En is highly conserved among distantly related Solanum species.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- VInvIn2Ene0
- Outcome
- highly conserved among distantly related Solanum speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the VInvIn2En sequence is highly conserved among distantly related Solanum species, including tomato (Solanum lycopersicum) and other non-tuber-bearing species.”
t0“potato”
Claim · model-written summary
The authors infer distinct cold stress roles for VInv and VInvIn2En in tuber-bearing Solanum tubers.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Gene
- VInv gene and the VInvIn2En enhancere0
- Outcome
- distinct roles in the cold stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that the VInv gene and the VInvIn2En enhancer have adopted distinct roles in the cold stress response in tubers of tuber-bearing Solanum species.”
t0“potato”
The same questions in other papers
loss of function · molecular: expression · Solanum tuberosum (potato)Positive and negative results
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