Abstract
Increasing evidence suggests that heat acclimation and exogenous salicylic acid (SA) and abscisic acid (ABA) may lead to the enhancement of thermotolerance in plants. In this study, the roles that free SA, conjugated SA, ABA, and phosphatidylinositol-4,5-bisphosphate (PIP(2))-specific phospholipase C (PLC) play in thermotolerance development induced by heat acclimation (38 degrees C) were investigated. To evaluate their potential functions, three inhibitors of synthesis or activity were infiltrated into pea leaves prior to heat acclimation treatment. The results showed that the burst of free SA in response to heat acclimation could be attributed to the conversion of SA 2-O-D-glucose, the main conjugated form of SA, to free SA. Inhibition of ABA biosynthesis also resulted in a defect in the free SA peak during heat acclimation. In acquired thermotolerance assessment, the greatest weakness of antioxidant enzyme activity and the most severe heat injury (malondialdehyde content and degree of wilting) were found in pea leaves pre-treated with neomycin, a well-known inhibitor of PIP(2)-PLC activity. PsPLC gene expression was activated by exogenous ABA, SA treatments, and heat acclimation after pre-treatments with a SA biosynthesis inhibitor. From these results, PIP(2)-PLC appears to play a key role in free SA- and ABA-associated reinforcement of thermotolerance resulting from heat acclimation.
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Claims 6
Claim · model-written summary
The burst of free SA during heat acclimation may be attributable to conversion of SA 2-O-D-glucose to free SA in pea leaves.
- Taxon
- pea
Lathyrus oleraceust0 - Compound
- SA 2-O-D-glucosee1
- Outcome
- burst of free SA in response to heat acclimatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e1“the burst of free SA in response to heat acclimation could be attributed to the conversion of SA 2-O-D-glucose, the main conjugated form of SA, to free SA”
Claim · model-written summary
Inhibition of ABA biosynthesis produced a defect in the free SA peak during heat acclimation in pea leaves.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- Inhibition of ABA biosynthesise2
- Outcome
- free SA peak during heat acclimatione2
- Direction
- defecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e2“Inhibition of ABA biosynthesis also resulted in a defect in the free SA peak during heat acclimation”
Claim · model-written summary
Pre-treatment with neomycin gave the greatest weakness of antioxidant enzyme activity in heat-acclimated pea leaves.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- neomycine3
- Outcome
- antioxidant enzyme activitye3
- Direction
- greatest weaknesse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e3“the greatest weakness of antioxidant enzyme activity and the most severe heat injury (malondialdehyde content and degree of wilting) were found in pea leaves pre-treated with neomycin, a well-known inhibitor of PIP(2)-PLC activity”
Claim · model-written summary
Pre-treatment with neomycin gave the most severe heat injury (malondialdehyde content and wilting) in pea leaves.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- neomycine3
- Outcome
- heat injury (malondialdehyde content and degree of wilting)e3
- Direction
- most severee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e3“the greatest weakness of antioxidant enzyme activity and the most severe heat injury (malondialdehyde content and degree of wilting) were found in pea leaves pre-treated with neomycin, a well-known inhibitor of PIP(2)-PLC activity”
Claim · model-written summary
PsPLC gene expression was activated by exogenous ABA, SA treatments and heat acclimation after SA biosynthesis inhibitor pre-treatment in pea.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- exogenous ABA, SA treatments, and heat acclimatione4
- Gene
- PsPLCe4
- Outcome
- PsPLC gene expressione4
- Direction
- activatede4
- Qualifier
- after pre-treatments with a SA biosynthesis inhibitore4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e4“PsPLC gene expression was activated by exogenous ABA, SA treatments, and heat acclimation after pre-treatments with a SA biosynthesis inhibitor”
Claim · model-written summary
PIP(2)-PLC appears to play a key role in SA- and ABA-associated reinforcement of thermotolerance from heat acclimation in pea.
- Taxon
- peaLathyrus oleraceust0
- Gene
- PIP(2)-PLCe5
- Outcome
- thermotolerancee5
- Qualifier
- free SA- and ABA-associated reinforcemente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea leaves”
e5“PIP(2)-PLC appears to play a key role in free SA- and ABA-associated reinforcement of thermotolerance resulting from heat acclimation”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- no effect
(+)-abscisic acid · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
salicylic acid · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
heat · molecular: expression · Lathyrus oleraceus (pea)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.