Abstract
The relationships between organogenesis of oil flax ( Linum usitatissimum L., cv. ‘Szafir’) in vitro, cyanogenic potential (HCN-p) of these tissues and light were investigated. Shoot multiplication obtained on Murashige and Skoog medium containing 0.05 mg L −1 2,4-dichloro-phenoxyacetic acid and 1 mg L −1 6-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness. Light-grown explants showed also higher rate of roots regeneration (in medium containing 1 mg L −1 α-naphtaleneacetic acid and 0.05 mg L -1 BA) than dark-grown ones. The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness. The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots, and its amount was twice as high as in roots, and about threefold higher than in callus tissue. The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness. Thus, higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues. We suggest that free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency.
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Claims 12
Claim · model-written summary
Shoot multiplication was about twice as high in light as darkness.
- Taxon
- Linum usitatissimum L.
Linum usitatissimumt0 - Treatment
- light-grown culturese0
- Outcome
- Shoot multiplicatione0
- Direction
- highere0
- Value
- about twicee0
- Comparator
- those in darknesse0
- Setting
- Murashige and Skoog mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot multiplication obtained on Murashige and Skoog medium containing 0.05 mg L −1 2,4-dichloro-phenoxyacetic acid and 1 mg L −1 6-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness”
t0“Linum usitatissimum L.”
Claim · model-written summary
Light grown explants had higher root regeneration than dark grown explants.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Treatment
- Light-grown explantse0
- Outcome
- rate of roots regeneratione0
- Direction
- highere0
- Comparator
- dark-grown onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light-grown explants showed also higher rate of roots regeneration (in medium containing 1 mg L −1 α-naphtaleneacetic acid and 0.05 mg L -1 BA) than dark-grown ones”
t0“Linum usitatissimum L.”
Claim · model-written summary
Cyanogenic potential varied by tissue.
- Taxon
- flaxLinum usitatissimume0
- Outcome
- cyanogenic potentiale0
- Qualifier
- tissue-specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific”
Claim · model-written summary
Cyanogenic potential was generally higher in light than darkness.
- Taxon
- flaxLinum usitatissimume0
- Treatment
- light conditionse0
- Outcome
- cyanogenic potentiale0
- Direction
- highere0
- Qualifier
- generallye0
- Comparator
- darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness”
Claim · model-written summary
Linamarin concentration was highest in light regenerated shoots.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Organ / stage
- light-regenerated shootse0
- Outcome
- concentration of linamarine0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots”
t0“Linum usitatissimum L.”
Claim · model-written summary
Lotaustralin concentration was highest in light regenerated shoots.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Organ / stage
- light-regenerated shootse0
- Outcome
- lotaustraline0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots”
t0“Linum usitatissimum L.”
Claim · model-written summary
Cyanogenic compounds in shoots were twice the root amount.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Outcome
- amounte0
- Direction
- highe0
- Value
- twicee0
- Comparator
- rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its amount was twice as high as in roots”
t0“Linum usitatissimum L.”
Claim · model-written summary
Cyanogenic compounds in shoots were about threefold the callus amount.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Outcome
- about threefold highere0
- Direction
- highere0
- Value
- about threefolde0
- Comparator
- callus tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“about threefold higher than in callus tissue”
t0“Linum usitatissimum L.”
Claim · model-written summary
Linamarase activity was higher in light regenerated organs than darkness.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Treatment
- light-regenerated organse0
- Gene
- linamarasee0
- Outcome
- activities of linamarasee0
- Direction
- highere0
- Comparator
- those in darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness”
t0“Linum usitatissimum L.”
Claim · model-written summary
β-cyanoalanine synthase activity was higher in light regenerated organs than darkness.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Treatment
- light-regenerated organse0
- Gene
- β-cyanoalanine synthasee0
- Outcome
- β-cyanoalanine synthasee0
- Direction
- highere0
- Comparator
- those in darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness”
t0“Linum usitatissimum L.”
Claim · model-written summary
Regeneration frequency was correlated with cyanogenic potential.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Organ / stage
- tissuese0
- Outcome
- frequency of regeneratione0
- Direction
- highere0
- Qualifier
- seems to be correlated with higher HCN-pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues”
t0“Linum usitatissimum L.”
Claim · model-written summary
The authors propose free HCN as a contributor to organogenesis and regeneration.
- Taxon
- Linum usitatissimum L.Linum usitatissimumt0
- Compound
- free HCNe0
- Outcome
- regeneration efficiencye0
- Qualifier
- may be involved in some organogenetic processese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency”
t0“Linum usitatissimum L.”
The same questions in other papers
light · growth · Linum usitatissimum (flax)Results differ
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
1 paper more in this group
See all 5 papers →in vitro · growth · Linum usitatissimum (flax)Positive and negative results
8 papers in the same direction as this paper · 2 in the opposite direction
- positive
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- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.