Abstract
Cadmium (Cd) is a widespread pollutant that is toxic to plant growth. However, only a few genes that contribute to Cd resistance in plants have been identified. To identify additional Cd(II) resistance genes, we screened an Arabidopsis cDNA library using a yeast (Saccharomyces cerevisiae) expression system employing the Cd(II)-sensitive yeast mutant ycf1. This screening process yielded a small Cys-rich membrane protein (Arabidopsis plant cadmium resistance, AtPcrs). Database searches revealed that there are nine close homologs in Arabidopsis. Homologs were also found in other plants. Four of the five homologs that were tested also increased resistance to Cd(II) when expressed in ycf1. AtPcr1 localizes at the plasma membrane in both yeast and Arabidopsis. Arabidopsis plants overexpressing AtPcr1 exhibited increased Cd(II) resistance, whereas antisense plants that showed reduced AtPcr1 expression were more sensitive to Cd(II). AtPcr1 overexpression reduced Cd uptake by yeast cells and also reduced the Cd contents of both yeast and Arabidopsis protoplasts treated with Cd. Thus, it appears that the Pcr family members may play an important role in the Cd resistance of plants.
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Claims 9
Claim · model-written summary
A screen identified Arabidopsis plant cadmium resistance proteins.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- Arabidopsis plant cadmium resistance, AtPcrse0
- Outcome
- small Cys-rich membrane proteine0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This screening process yielded a small Cys-rich membrane protein (Arabidopsis plant cadmium resistance, AtPcrs)”
Claim · model-written summary
Arabidopsis has nine close Pcr homologs.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- close homologse0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there are nine close homologs in Arabidopsis”
Claim · model-written summary
Four of five tested homologs increased cadmium resistance in yeast.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- expressed in ycf1e0
- Gene
- homologse0
- Outcome
- resistance to Cd(II)e0
- Direction
- increasede0
- Value
- Four of the fivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four of the five homologs that were tested also increased resistance to Cd(II) when expressed in ycf1”
t0“Arabidopsis”
Claim · model-written summary
AtPcr1 localized at the plasma membrane in yeast and Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtPcr1e0
- Outcome
- localizes at the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPcr1 localizes at the plasma membrane in both yeast and Arabidopsis”
Claim · model-written summary
AtPcr1 overexpression increased Arabidopsis cadmium resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing AtPcr1e0
- Gene
- AtPcr1e0
- Outcome
- Cd(II) resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis plants overexpressing AtPcr1 exhibited increased Cd(II) resistance”
Claim · model-written summary
Reduced AtPcr1 expression increased cadmium sensitivity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- antisense plantse0
- Gene
- AtPcr1e0
- Outcome
- sensitive to Cd(II)e0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antisense plants that showed reduced AtPcr1 expression were more sensitive to Cd(II)”
t0“Arabidopsis”
Claim · model-written summary
AtPcr1 overexpression reduced cadmium uptake in yeast.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- AtPcr1 overexpressione0
- Gene
- AtPcr1e0
- Outcome
- Cd uptake by yeast cellse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtPcr1 overexpression reduced Cd uptake by yeast cells”
t0“Arabidopsis”
Claim · model-written summary
AtPcr1 overexpression reduced cadmium content in yeast and Arabidopsis protoplasts.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- treated with Cde0
- Gene
- Cys-Rich Membrane Proteinst0
- Outcome
- Cd contents of both yeast and Arabidopsis protoplastse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced the Cd contents of both yeast and Arabidopsis protoplasts treated with Cd”
t0“Cys-Rich Membrane Proteins”
Claim · model-written summary
Pcr proteins may contribute to plant cadmium resistance.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- Pcr family memberse0
- Outcome
- Cd resistance of plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pcr family members may play an important role in the Cd resistance of plants”
t0“Arabidopsis”
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