Abstract
Plant infections by the soil bacterium Agrobacterium rhizogenes result in neoplastic disease with the formation of hairy roots at the site of infection. Expression of a set of oncogenes residing on the stably integrated T-DNA is responsible for the disease symptoms. Besides the rol (root locus) genes, which are essential for the formation of hairy roots, the open reading frame orf13 mediates cytokinin-like effects, suggesting an interaction with hormone signaling pathways. Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum). ORF13 has a retinoblastoma (RB)-binding motif and interacted with maize (Zea mays) RB in vitro, whereas ORF13, bearing a point mutation in the RB-binding motif (ORF13*), did not. Increased cell divisions in the vegetative shoot apical meristem and accelerated formation of leaf primordia were observed in plants expressing orf13, whereas the expression of orf13* had no influence on cell division rates in the shoot apical meristem, suggesting a role of RB in the regulation of the cell cycle in meristematic tissues. On the other hand, ectopic expression of LeT6 was not dependent on a functional RB-binding motif. Hormone homeostasis was only altered in explants of leaves, whereas in the root no effects were observed. We suggest that ORF13 confers meristematic competence to cells infected by A. rhizogenes by inducing the expression of KNOX genes and promotes the transition of infected cells from the G1 to the S phase by binding to RB.
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Claims 10
Claim · model-written summary
ORF13 induced ectopic expression of KNOX class transcription factors and several cell cycle control genes in tomato.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- ORF13e1
- Gene
- KNOXe1
- Outcome
- ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle controle1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
Claim · model-written summary
ORF13 interacted in vitro with maize (Zea mays) retinoblastoma (RB) protein.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“ORF13 has a retinoblastoma (RB)-binding motif and interacted with maize (Zea mays) RB in vitro, whereas ORF13, bearing a point mutation in the RB-binding motif (ORF13*), did not”
Claim · model-written summary
The point-mutated ORF13* (mutation in the RB-binding motif) did not interact with maize RB in vitro.
- Treatment
- point mutation in the RB-binding motife2
- Gene
- RBe2
- Outcome
- interacted with maize (Zea mays) RBe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“ORF13 has a retinoblastoma (RB)-binding motif and interacted with maize (Zea mays) RB in vitro, whereas ORF13, bearing a point mutation in the RB-binding motif (ORF13*), did not”
Claim · model-written summary
Plants expressing orf13 showed increased cell divisions in the vegetative shoot apical meristem.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- vegetative shoot apical meristeme3
- Treatment
- plants expressing orf13e3
- Outcome
- cell divisions in the vegetative shoot apical meristeme3
- Direction
- Increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e3“Increased cell divisions in the vegetative shoot apical meristem and accelerated formation of leaf primordia were observed in plants expressing orf13”
Claim · model-written summary
Plants expressing orf13 showed accelerated formation of leaf primordia.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- plants expressing orf13e3
- Outcome
- formation of leaf primordiae3
- Direction
- acceleratede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e3“Increased cell divisions in the vegetative shoot apical meristem and accelerated formation of leaf primordia were observed in plants expressing orf13”
Claim · model-written summary
The expression of orf13* had no influence on cell division rates in the shoot apical meristem.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- the expression of orf13*e4
- Outcome
- cell division rates in the shoot apical meristeme4
- Direction
- no influencee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e4“whereas the expression of orf13* had no influence on cell division rates in the shoot apical meristem”
Claim · model-written summary
Ectopic expression of LeT6 was not dependent on a functional RB-binding motif.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- a functional RB-binding motife5
- Gene
- LeT6e5
- Outcome
- ectopic expression of LeT6e5
- Direction
- not dependente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e5“ectopic expression of LeT6 was not dependent on a functional RB-binding motif”
Claim · model-written summary
Hormone homeostasis was altered only in leaf explants of tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- explants of leavese6
- Outcome
- Hormone homeostasise6
- Direction
- alterede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e6“Hormone homeostasis was only altered in explants of leaves, whereas in the root no effects were observed”
Claim · model-written summary
No hormone homeostasis effects were observed in tomato roots.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- roote6
- Outcome
- Hormone homeostasise6
- Direction
- no effectse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e6“Hormone homeostasis was only altered in explants of leaves, whereas in the root no effects were observed”
Claim · model-written summary
ORF13 is suggested to promote the transition of infected cells from G1 to S phase by binding RB.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- RBe7
- Outcome
- transition of infected cells from the G1 to the S phasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum)”
e7“promotes the transition of infected cells from the G1 to the S phase by binding to RB”
The same questions in other papers
transgenic · cytology · Solanum lycopersicum (tomato)Results agree
4 papers in the same direction as this paper
- negative
- no effect
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.