Abstract
CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana. The CRY2 protein is found primarily in the nucleus, and it is known to undergo blue light-dependent phosphorylation and degradation. However, the subcellular location where CRY2 exerts its function or undergoes blue light-dependent phosphorylation and degradation remains unclear. In this study, we analyzed the function and regulation of conditionally nuclear-localized CRY2. Our results show that CRY2 mediates blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation in the nucleus. Consistent with this result and a hypothesis that blue light-dependent phosphorylation is associated with CRY2 function, we demonstrate that CRY2 undergoes blue light-dependent phosphorylation in the nucleus. CRY2 phosphorylation is required for blue light-dependent CRY2 degradation, but only a limited quantity of CRY2 is phosphorylated at any given moment in seedlings exposed to blue light, which explains why continuous blue light illumination is required for CRY2 degradation. Finally, we showed that CRY2 is ubiquitinated in response to blue light and that ubiquitinated CRY2 is degraded by the 26S proteasome in the nucleus. These findings demonstrate that a photoreceptor can complete its posttranslational life cycle (from protein modification, to function, to degradation) inside the nucleus.
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Claims 8
Claim · model-written summary
CRY2 mediates blue light inhibition of hypocotyl elongation in the nucleus.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- hypocotyle1
- Treatment
- blue lighte1
- Gene
- CRY2e1
- Outcome
- hypocotyl elongatione1
- Direction
- inhibitione1
- Qualifier
- in the nucleuse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“Our results show that CRY2 mediates blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation in the nucleus”
Claim · model-written summary
CRY2 mediates photoperiodic promotion of floral initiation in the nucleus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- photoperiodice1
- Gene
- CRY2e1
- Outcome
- floral initiatione1
- Direction
- promotione1
- Qualifier
- in the nucleuse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“Our results show that CRY2 mediates blue light inhibition of hypocotyl elongation and photoperiodic promotion of floral initiation in the nucleus”
Claim · model-written summary
CRY2 undergoes blue light-dependent phosphorylation in the nucleus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- CRY2e1
- Outcome
- blue light-dependent phosphorylatione1
- Qualifier
- in the nucleuse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“we demonstrate that CRY2 undergoes blue light-dependent phosphorylation in the nucleus”
Claim · model-written summary
CRY2 phosphorylation is required for blue light-dependent CRY2 degradation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- CRY2e1
- Outcome
- blue light-dependent CRY2 degradatione1
- Qualifier
- CRY2 phosphorylation is requirede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“CRY2 phosphorylation is required for blue light-dependent CRY2 degradation”
Claim · model-written summary
Only a limited quantity of CRY2 is phosphorylated at any given moment in seedlings exposed to blue light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- seedlingse1
- Treatment
- blue lighte1
- Gene
- CRY2e1
- Outcome
- a limited quantity of CRY2 is phosphorylatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“only a limited quantity of CRY2 is phosphorylated at any given moment in seedlings exposed to blue light”
Claim · model-written summary
Continuous blue light illumination is required for CRY2 degradation (author interpretation).
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- continuous blue light illuminatione1
- Gene
- CRY2e1
- Outcome
- CRY2 degradatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“continuous blue light illumination is required for CRY2 degradation”
Claim · model-written summary
CRY2 is ubiquitinated in response to blue light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- blue lighte1
- Gene
- CRY2e1
- Outcome
- CRY2 is ubiquitinatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“we showed that CRY2 is ubiquitinated in response to blue light”
Claim · model-written summary
Ubiquitinated CRY2 is degraded by the 26S proteasome in the nucleus.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- CRY2e1
- Outcome
- ubiquitinated CRY2 is degradede1
- Qualifier
- by the 26S proteasomee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRY2 is a blue light receptor regulating light inhibition of hypocotyl elongation and photoperiodic flowering in Arabidopsis thaliana”
e1“ubiquitinated CRY2 is degraded by the 26S proteasome in the nucleus”
The same questions in other papers
light · growth · Arabidopsis thaliana (thale cress)Results differ
29 papers in the same direction as this paper · 14 in the opposite direction
- negative
- negative
- negative
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See all 44 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.