Abstract
The formation of tryptamine from tryptophan by extracts of cucumber hypocotyls is mediated by a tryptophan decarboxylase. The enzyme requires pyridoxal-5′-phosphate, and the pH optimum of this enzyme is 7.0. The activity of the enzyme is inhibited by potassium cyanide, but not by sodium azide or sodium fluoride; indicating that this enzyme is a decarboxylase rather than a peroxidase. The removal of contaminating epiphytic bacteria does not significantly affect the enzyme activity, and preincubation of enzyme extracts in streptomycin is also without effect. Neither aerobic nor anaerobic cultures of internal bacteria which contaminate cucumber hypocotyb exhibit enzymic activity at pH 7.0.
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Claims 9
Claim · model-written summary
A tryptophan decarboxylase mediates tryptamine formation from tryptophan in cucumber hypocotyl extracts.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- hypocotylse0
- Gene
- tryptophan decarboxylasee0
- Outcome
- formation of tryptamine from tryptophane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The formation of tryptamine from tryptophan by extracts of cucumber hypocotyls is mediated by a tryptophan decarboxylase”
Claim · model-written summary
The enzyme requires pyridoxal-5′-phosphate.
- Taxon
- cucumberCucumis sativust0
- Compound
- pyridoxal-5′-phosphatee0
- Outcome
- requires pyridoxal-5′-phosphatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme requires pyridoxal-5′-phosphate”
t0“cucumber seedlings”
Claim · model-written summary
The enzyme has a pH optimum of 7.0.
- Taxon
- cucumberCucumis sativust0
- Outcome
- pH optimume0
- Value
- 7.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pH optimum of this enzyme is 7.0”
t0“cucumber seedlings”
Claim · model-written summary
Potassium cyanide inhibited enzyme activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- potassium cyanidee0
- Outcome
- activity of the enzymee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme is inhibited by potassium cyanide”
t0“cucumber seedlings”
Claim · model-written summary
Sodium azide did not inhibit enzyme activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- sodium azidee0
- Outcome
- activity of the enzymee0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme is inhibited by potassium cyanide, but not by sodium azide or sodium fluoride”
t0“cucumber seedlings”
Claim · model-written summary
Sodium fluoride did not inhibit enzyme activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- sodium fluoridee0
- Outcome
- activity of the enzymee0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of the enzyme is inhibited by potassium cyanide, but not by sodium azide or sodium fluoride”
t0“cucumber seedlings”
Claim · model-written summary
Removing epiphytic bacteria did not significantly affect enzyme activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- removal of contaminating epiphytic bacteriae0
- Outcome
- enzyme activitye0
- Direction
- does not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The removal of contaminating epiphytic bacteria does not significantly affect the enzyme activity”
t0“cucumber seedlings”
Claim · model-written summary
Preincubating extracts in streptomycin had no effect.
- Taxon
- cucumberCucumis sativust0
- Treatment
- preincubation of enzyme extracts in streptomycine0
- Outcome
- without effecte0
- Direction
- without effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“preincubation of enzyme extracts in streptomycin is also without effect”
t0“cucumber seedlings”
Claim · model-written summary
Internal bacteria cultures did not show enzyme activity at pH 7.0.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- hypocotybe0
- Outcome
- enzymic activitye0
- Qualifier
- at pH 7.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither aerobic nor anaerobic cultures of internal bacteria which contaminate cucumber hypocotyb exhibit enzymic activity at pH 7.0”
The same questions in other papers
inoculation · soil: biology · Cucumis sativus (cucumber)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.