Abstract
LeAGP-1 is a glycosylphosphatidylinositol (GPI)-anchored arabinogalactan-protein (AGP) in tomato (Lycopersicon esculentum). Patterns of mRNA expression and protein localization for LeAGP-1 indicate that it likely functions in certain aspects of plant growth and development. To elucidate LeAGP-1 function(s), transgenic tomato plants expressing enhanced green fluorescent protein (GFP) fused to LeAGP-1 [GFP-LeAGP-1] or two LeAGP-1 variants, one lacking the C-terminal GPI-anchor domain [GFP-LeAGP-1DeltaC] and the other lacking the lysine-rich domain [GFP-LeAGP-1DeltaK], under the control of the CaMV35S promoter were produced using Agrobacterium-mediated transformation. Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein: (i) were significantly shorter; (ii) were highly branched; (iii) produced more flower buds, but most of these flowers did not mature, resulting in less fruit production; and (iv) produced seeds that were significantly smaller than normal seeds. Overexpression of LeAGP-1DeltaK had a similar or even more pronounced effect on plant vegetative and reproductive growth, while the effect of LeAGP-1DeltaC overexpression on plant reproduction was minimal. These results indicate that the GPI anchor is critical for LeAGP-1 function. As the phenotype of GFP-LeAGP-1 overexpressing transgenic plants is similar to that of cytokinin-overproducing plants, mRNA expression patterns of LeAGP-1 under different hormone treatments were examined. Cytokinins upregulated LeAGP-1 mRNA expression, while auxins and ABA inhibited LeAGP-1 mRNA expression. Based on these results, GPI-anchored LeAGP-1 most likely functions in plant growth and development in concert with auxin/cytokinin signaling.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Tomato lines with high GFP-LeAGP-1 expression were significantly shorter.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Gene
- GFP-LeAGP-1e1
- Outcome
- shortere2
- Direction
- shortere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e2“(i) were significantly shorter”
Claim · model-written summary
Tomato lines with high GFP-LeAGP-1 expression were highly branched.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Outcome
- lateral branchingt1
- Direction
- promotest1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
t1“promotes lateral branching”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e3“(ii) were highly branched”
Claim · model-written summary
Tomato lines with high GFP-LeAGP-1 expression produced more flower buds.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Outcome
- flower budse4
- Direction
- moree4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e4“(iii) produced more flower buds”
Claim · model-written summary
Tomato lines with high GFP-LeAGP-1 expression produced less fruit.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Outcome
- fruit productione5
- Direction
- lesse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e5“resulting in less fruit production”
Claim · model-written summary
Most flowers on tomato lines with high GFP-LeAGP-1 expression did not mature.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Outcome
- flowers did not maturee6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e6“most of these flowers did not mature”
Claim · model-written summary
Tomato lines with high GFP-LeAGP-1 expression produced significantly smaller seeds than normal seeds.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- high levels of both GFP-LeAGP-1 mRNA and proteine1
- Outcome
- seedse7
- Direction
- smallere7
- Comparator
- normal seedse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e1“Transgenic T0 and T1 lines with high levels of both GFP-LeAGP-1 mRNA and protein”
e7“(iv) produced seeds that were significantly smaller than normal seeds”
Claim · model-written summary
LeAGP-1 lacking the lysine-rich domain had a similar or more pronounced effect on vegetative and reproductive growth.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Overexpression of LeAGP-1DeltaKe7
- Gene
- LeAGP-1DeltaKe7
- Outcome
- plant vegetative and reproductive growthe7
- Direction
- similar or even more pronouncede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“Overexpression of LeAGP-1DeltaK had a similar or even more pronounced effect on plant vegetative and reproductive growth”
Claim · model-written summary
LeAGP-1 lacking the GPI anchor domain had a minimal effect on plant reproduction.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- LeAGP-1DeltaC overexpressione7
- Gene
- LeAGP-1DeltaCe7
- Outcome
- plant reproductione7
- Direction
- minimale7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“the effect of LeAGP-1DeltaC overexpression on plant reproduction was minimal”
Claim · model-written summary
Cytokinin treatment upregulated LeAGP-1 mRNA expression in tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Cytokininse7
- Gene
- LeAGP-1e7
- Outcome
- LeAGP-1 mRNA expressione7
- Direction
- upregulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“Cytokinins upregulated LeAGP-1 mRNA expression”
Claim · model-written summary
Auxin treatment inhibited LeAGP-1 mRNA expression in tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- auxinse7
- Gene
- LeAGP-1e7
- Outcome
- LeAGP-1 mRNA expressione7
- Direction
- inhibitede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“while auxins and ABA inhibited LeAGP-1 mRNA expression”
Claim · model-written summary
ABA treatment inhibited LeAGP-1 mRNA expression in tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- ABAe7
- Gene
- LeAGP-1e7
- Outcome
- LeAGP-1 mRNA expressione7
- Direction
- inhibitede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“while auxins and ABA inhibited LeAGP-1 mRNA expression”
Claim · model-written summary
The GPI anchor is critical for LeAGP-1 function in tomato.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- LeAGP-1e7
- Outcome
- LeAGP-1 functione7
- Qualifier
- the GPI anchor is criticale7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“These results indicate that the GPI anchor is critical for LeAGP-1 function”
Claim · model-written summary
GPI-anchored LeAGP-1 likely functions in plant growth and development together with auxin/cytokinin signaling.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- GPI-anchored LeAGP-1e7
- Outcome
- plant growth and developmente7
- Qualifier
- in concert with auxin/cytokinin signalinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“GPI-anchored LeAGP-1 most likely functions in plant growth and development in concert with auxin/cytokinin signaling”
Claim · model-written summary
Tomato plants overexpressing GFP-LeAGP-1 have a phenotype similar to cytokinin-overproducing plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- GFP-LeAGP-1 overexpressing transgenic plantse7
- Outcome
- phenotypee7
- Direction
- similare7
- Comparator
- cytokinin-overproducing plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Overexpression of tomato”
e7“As the phenotype of GFP-LeAGP-1 overexpressing transgenic plants is similar to that of cytokinin-overproducing plants”
The same questions in other papers
overexpression · growth · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.