Abstract
Abstract The most common way to test seed quality is to use a simple and reliable but time- and space-consuming germination test. In this paper we present a fast and simple method to analyse cabbage seed deterioration by measuring ethanol production from partially imbibed seeds. The method uses a modified breath analyser and is simple compared to gas chromatographic or enzymatic procedures. A modified method using elevated temperatures (40°C instead of 20°C) shortened the assay time and improved its sensitivity. The analysis showed an inverse correlation between ethanol production and seed quality (e.g. the final percentages or speed of germination and the number of normal seedlings). The increase in ethanol production was observed when cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings. Premature seeds produced more ethanol upon imbibition than mature seeds. Ethanol production occurred simultaneously with oxygen consumption, indicating that lack of oxygen is not the major trigger for ethanol production.
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Claims 10
Claim · model-written summary
Running the ethanol assay at 40°C rather than 20°C shortened assay time.
- Taxon
- cabbage
Brassica oleracea var. capitatae1 - Treatment
- 40°Ce0
- Outcome
- assay timee0
- Direction
- shortenede0
- Value
- 40°Ce0
- Comparator
- 20°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A modified method using elevated temperatures (40°C instead of 20°C) shortened the assay time and improved its sensitivity.”
e1“cabbage seed deterioration”
Claim · model-written summary
Running the ethanol assay at 40°C rather than 20°C improved sensitivity.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Outcome
- sensitivitye0
- Direction
- improvede0
- Value
- 40°Ce0
- Comparator
- 20°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A modified method using elevated temperatures (40°C instead of 20°C) shortened the assay time and improved its sensitivity.”
e1“cabbage seed deterioration”
Claim · model-written summary
Ethanol production was inversely correlated with final germination percentage, germination speed and normal seedling number.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Compound
- ethanole0
- Outcome
- seed qualitye0
- Direction
- inversee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The analysis showed an inverse correlation between ethanol production and seed quality (e.g. the final percentages or speed of germination and the number of normal seedlings).”
e1“cabbage seed deterioration”
Claim · model-written summary
Ambient storage deterioration increased cabbage seed ethanol production.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Treatment
- storage under ambient conditionse0
- Outcome
- ethanol productione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in ethanol production was observed when cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings.”
Claim · model-written summary
Hot water deterioration increased cabbage seed ethanol production.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Treatment
- hot water treatmentse0
- Outcome
- ethanol productione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in ethanol production was observed when cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings.”
Claim · model-written summary
Ambient storage deterioration reduced the number of normal cabbage seedlings.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Treatment
- storage under ambient conditionse0
- Outcome
- number of normal seedlingse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings.”
Claim · model-written summary
Hot water deterioration reduced the number of normal cabbage seedlings.
- Taxon
- cabbageBrassica oleracea var. capitatae0
- Treatment
- hot water treatmentse0
- Outcome
- number of normal seedlingse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings.”
Claim · model-written summary
Premature seeds produced more ethanol upon imbibition than mature seeds.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Organ / stage
- Premature seedse0
- Outcome
- ethanole0
- Direction
- moree0
- Comparator
- mature seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Premature seeds produced more ethanol upon imbibition than mature seeds.”
e1“cabbage seed deterioration”
Claim · model-written summary
Ethanol production and oxygen consumption occurred simultaneously upon seed imbibition.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Outcome
- Ethanol production occurred simultaneously with oxygen consumptione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethanol production occurred simultaneously with oxygen consumption”
e1“cabbage seed deterioration”
Claim · model-written summary
The authors infer oxygen scarcity is not the main ethanol production trigger.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Outcome
- ethanol productione0
- Qualifier
- lack of oxygen is not the major triggere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating that lack of oxygen is not the major trigger for ethanol production.”
e1“cabbage seed deterioration”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.