Abstract
Soybean seeds [Glycine max (L.) Merr. ev. Bragg] were mutagenized with ethyl methanesulfonate. The M(2) progeny (i.e., the first generation after mutagenesis) of these seeds were screened for increased nodulation under high nitrate culture conditions. Fifteen independent nitrate-tolerant symbiotic (nts) mutants were obtained from 2500 M(2) families. In culture on sand with KNO(3), nodule mass and nodule number in mutant lines were several-fold those of the wild type cultured under the same conditions. Inheritance of the nts character through to subsequent generations was observed in the 10 mutants tested. Mutant nts382 also nodulated more than the wild type in the absence of nitrate. Furthermore, nitrate stimulated growth in both the wild type and nts382, and these lines had similar nitrate reductase activity. These results indicate that nts382 is affected in a nodule-development regulatory gene and not in a gene related to nitrate assimilation.
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Claims 9
Claim · model-written summary
Fifteen independent nitrate-tolerant symbiotic mutants were recovered from 2500 M(2) families.
- Taxon
- Soybean
Glycine maxe1 - Material
- nitrate-tolerant symbiotic (nts) mutantse0
- Outcome
- mutants were obtainede0
- Value
- Fifteene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifteen independent nitrate-tolerant symbiotic (nts) mutants were obtained from 2500 M(2) families.”
e1“Soybean seeds”
Claim · model-written summary
Mutant lines had several-fold greater nodule mass than wild type on sand with KNO(3).
- Taxon
- SoybeanGlycine maxe1
- Material
- mutant linese0
- Treatment
- KNO(3)e0
- Outcome
- nodule masse0
- Direction
- several-folde0
- Value
- several-folde0
- Comparator
- wild typee0
- Setting
- sande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In culture on sand with KNO(3), nodule mass and nodule number in mutant lines were several-fold those of the wild type cultured under the same conditions.”
e1“Soybean seeds”
Claim · model-written summary
Mutant lines had several-fold more nodules than wild type on sand with KNO(3).
- Taxon
- SoybeanGlycine maxe1
- Material
- mutant linese0
- Treatment
- KNO(3)e0
- Outcome
- nodule numbere0
- Direction
- several-folde0
- Value
- several-folde0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In culture on sand with KNO(3), nodule mass and nodule number in mutant lines were several-fold those of the wild type cultured under the same conditions.”
e1“Soybean seeds”
Claim · model-written summary
The nts character was inherited in the 10 mutants tested.
- Taxon
- SoybeanGlycine maxe1
- Material
- ntse0
- Outcome
- Inheritance of the nts charactere0
- Value
- 10 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inheritance of the nts character through to subsequent generations was observed in the 10 mutants tested.”
e1“Soybean seeds”
Claim · model-written summary
nts382 formed more nodules than wild type without nitrate.
- Taxon
- SoybeanGlycine maxe1
- Material
- nts382e0
- Treatment
- absence of nitratee0
- Outcome
- nodulatede0
- Direction
- moree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant nts382 also nodulated more than the wild type in the absence of nitrate.”
e1“Soybean seeds”
Claim · model-written summary
Nitrate stimulated growth in wild type soybean.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate stimulated growth in both the wild type and nts382”
e1“Soybean seeds”
Claim · model-written summary
Nitrate stimulated growth in nts382 soybean.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate stimulated growth in both the wild type and nts382”
e1“Soybean seeds”
Claim · model-written summary
Wild type and nts382 had similar nitrate reductase activity.
- Taxon
- SoybeanGlycine maxe2
- Material
- nts382e1
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- similare0
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these lines had similar nitrate reductase activity”
e1“both the wild type and nts382”
e2“Soybean seeds”
Claim · model-written summary
The authors infer nts382 affects a nodule-development regulatory gene.
- Taxon
- SoybeanGlycine maxe1
- Material
- nts382e0
- Gene
- nodule-development regulatory genee0
- Outcome
- nodule-development regulatory genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nts382 is affected in a nodule-development regulatory gene and not in a gene related to nitrate assimilation”
e1“Soybean seeds”
The same questions in other papers
nitrogen · growth · Glycine max (soybean)Positive and negative results
116 papers in the same direction as this paper · 34 in the opposite direction · 2 reporting no difference from an alternative
- negative
- positive
- negative
149 papers more in this group
See all 153 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.