Abstract
Potato plantlets inoculated with strain PsJN of the bacterium Burkholderia phytofirmans exhibit consistent and significant increases in plant growth under in vitro conditions, when compared with uninoculated plants. The greatest influence on the degree and type of growth enhancement that develops has been shown to be mediated by the sugar concentration in the agar media. Bacterial growth promotion has been suggested in other studies to be regulated by the sugar sensor enzyme hexokinase1, the role of which is activation of glucose phosphorylation. In this present study, we examined the co-relationship between root and stem development in potato plants treated with PsJN and the activity of hexokinase1. Plants grown in the presence of 1.5% and 3% sucrose showed increased levels of hexokinase1 activity only in the roots of inoculated plants, suggesting that the increased enzyme levels may be associated with root growth. Analysis for mRNA using reverse transcriptase did not reveal any significant differences in transcription levels of the gene between inoculated and uninoculated plants. When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased. No growth promotion occurred when PsJN-inoculated plants were grown in 3% maltose. Subsequently, a hexokinase1 activity assay showed that PsJN-induced growth of potato plants was found to only occur when plants were grown in the presence of sugars that are recognized by the plant hexokinase1. The results suggest that PsJN may enhance sugar uptake in plants by direct or indirect stimulation of hexokinase1 activity in roots and this results in enhanced overall plant growth.
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Claims 11
Claim · model-written summary
Inoculated potato plantlets had significantly increased growth relative to uninoculated plants in vitro.
- Taxon
- Potato
Solanum tuberosume0 - Treatment
- inoculated with strain PsJNe0
- Interactor
- Burkholderia phytofirmansParaburkholderia phytofirmanse0symbiont
- Outcome
- plant growthe0
- Direction
- increasese0
- Comparator
- uninoculated plantse0
- Setting
- in vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Potato plantlets inoculated with strain PsJN of the bacterium Burkholderia phytofirmans exhibit consistent and significant increases in plant growth under in vitro conditions, when compared with uninoculated plants.”
Claim · model-written summary
Sugar concentration in agar media had the greatest influence on the degree and type of bacterial growth enhancement.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- sugar concentration in the agar mediae0
- Interactor
- PsJNe1symbiont
- Outcome
- growth enhancemente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The greatest influence on the degree and type of growth enhancement that develops has been shown to be mediated by the sugar concentration in the agar media.”
e1“potato plants treated with PsJN”
Claim · model-written summary
At 1.5% and 3% sucrose, hexokinase1 activity increased only in roots of inoculated plants.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- rootse0
- Treatment
- 1.5% and 3% sucrosee0
- Interactor
- PsJNe1symbiont
- Gene
- hexokinase1e0
- Outcome
- hexokinase1 activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants grown in the presence of 1.5% and 3% sucrose showed increased levels of hexokinase1 activity only in the roots of inoculated plants”
e1“potato plants treated with PsJN”
Claim · model-written summary
Hexokinase1 transcription did not differ significantly between inoculated and uninoculated plants.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- inoculatede0
- Interactor
- PsJNe1symbiont
- Gene
- hexokinase1e2
- Outcome
- transcription levels of the genee0
- Direction
- did not reveal any significant differencese0
- Comparator
- uninoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis for mRNA using reverse transcriptase did not reveal any significant differences in transcription levels of the gene between inoculated and uninoculated plants.”
e1“potato plants treated with PsJN”
e2“activity of hexokinase1”
Claim · model-written summary
With 1.5% and 3% glucose, PsJN-inoculated potato plants increased in stem height and mass and leaf number.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- 1.5% and 3% concentrations of glucosee0
- Interactor
- PsJNe0symbiont
- Outcome
- stem height and mass, leaf numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased.”
e1“potato plants treated with PsJN”
Claim · model-written summary
With 1.5% and 3% fructose, PsJN-inoculated potato plants increased in stem height and mass and leaf number.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- fructosee0
- Interactor
- PsJNe0symbiont
- Outcome
- stem height and mass, leaf numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased.”
e1“potato plants treated with PsJN”
Claim · model-written summary
With 1.5% and 3% glucose, PsJN-inoculated potato plants increased in root mass and overall biomass.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- 1.5% and 3% concentrations of glucosee0
- Interactor
- PsJNe0symbiont
- Outcome
- root mass, and overall biomasse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased.”
e1“potato plants treated with PsJN”
Claim · model-written summary
With 1.5% and 3% fructose, PsJN-inoculated potato plants increased in root mass and overall biomass.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- fructosee0
- Interactor
- PsJNe0symbiont
- Outcome
- root mass, and overall biomasse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When PsJN-inoculated plants were grown in 1.5% and 3% concentrations of glucose and fructose, stem height and mass, leaf number, root mass, and overall biomass increased.”
e1“potato plants treated with PsJN”
Claim · model-written summary
PsJN-inoculated potato plants showed no growth promotion at 3% maltose.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- 3% maltosee0
- Interactor
- PsJNe0symbiont
- Outcome
- growth promotione0
- Direction
- No growth promotione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No growth promotion occurred when PsJN-inoculated plants were grown in 3% maltose.”
e1“potato plants treated with PsJN”
Claim · model-written summary
PsJN-induced potato growth occurred only with sugars recognized by plant hexokinase1.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- sugars that are recognized by the plant hexokinase1e0
- Interactor
- PsJNe0symbiont
- Gene
- plant hexokinase1e0
- Outcome
- PsJN-induced growthe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsJN-induced growth of potato plants was found to only occur when plants were grown in the presence of sugars that are recognized by the plant hexokinase1.”
Claim · model-written summary
The authors suggest PsJN enhances sugar uptake through stimulation of root hexokinase1 activity, enhancing overall growth.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- rootse0
- Interactor
- PsJNe0symbiont
- Gene
- hexokinase1e0
- Outcome
- enhanced overall plant growthe0
- Qualifier
- direct or indirect stimulation of hexokinase1 activity in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggest that PsJN may enhance sugar uptake in plants by direct or indirect stimulation of hexokinase1 activity in roots and this results in enhanced overall plant growth.”
e1“potato plants treated with PsJN”
The same questions in other papers
glucose · growth · Solanum tuberosum (potato)Positive and negative results
1 in the opposite direction
- no effect
maltose · growth · Solanum tuberosum (potato)Positive and negative results
2 in the opposite direction
- positive
- positive
inoculation · molecular: expression · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.