Abstract
The S-like ribonucleases (RNases) RNS1 and RNS2 of Arabidopsis are members of the widespread T2 ribonuclease family, whose members also include the S-RNases, involved in gametophytic self-incompatibility in plants. Both RNS1 and RNS2 mRNAs have been shown previously to be induced by inorganic phosphate (Pi) starvation. In our study we examined this regulation at the protein level and determined the effects of diminishing RNS1 and RNS2 expression using antisense techniques. The Pi-starvation control of RNS1 and RNS2 was confirmed using antibodies specific for each protein. These specific antibodies also demonstrated that RNS1 is secreted, whereas RNS2 is intracellular. By introducing antisense constructs, mRNA accumulation was inhibited by up to 90% for RNS1 and up to 65% for RNS2. These plants contained abnormally high levels of anthocyanins, the production of which is often associated with several forms of stress, including Pi starvation. This effect demonstrates that diminishing the amounts of either RNS1 or RNS2 leads to effects that cannot be compensated for by the actions of other RNases, even though Arabidopsis contains a large number of different RNase activities. These results, together with the differential localization of the proteins, imply that RNS1 and RNS2 have distinct functions in the plant.
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Claims 8
Claim · model-written summary
Phosphate starvation regulation of RNS1 and RNS2 proteins was confirmed.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- Pi-starvatione0
- Gene
- RNS1 and RNS2e0
- Outcome
- control of RNS1 and RNS2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Pi-starvation control of RNS1 and RNS2 was confirmed using antibodies specific for each protein”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
RNS1 was found to be secreted.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RNS1e0
- Outcome
- secretede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These specific antibodies also demonstrated that RNS1 is secreted, whereas RNS2 is intracellular”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
RNS2 was found to be intracellular.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RNS2e0
- Outcome
- intracellulare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These specific antibodies also demonstrated that RNS1 is secreted, whereas RNS2 is intracellular”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
Antisense constructs inhibited RNS1 mRNA accumulation by up to 90%.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- antisense constructse0
- Gene
- RNS1e0
- Outcome
- mRNA accumulatione0
- Direction
- inhibitede0
- Value
- 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By introducing antisense constructs, mRNA accumulation was inhibited by up to 90% for RNS1 and up to 65% for RNS2”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
Antisense constructs inhibited RNS2 mRNA accumulation by up to 65%.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- antisense constructse0
- Gene
- RNS2e0
- Outcome
- mRNA accumulatione0
- Direction
- inhibitede0
- Value
- 65%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By introducing antisense constructs, mRNA accumulation was inhibited by up to 90% for RNS1 and up to 65% for RNS2”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
Plants with reduced RNS1 expression accumulated abnormally high anthocyanins.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Antisense Inhibition of RNS1t0
- Gene
- RNS1t0
- Outcome
- levels of anthocyaninse0
- Direction
- abnormally highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants contained abnormally high levels of anthocyanins”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
Plants with reduced RNS2 expression accumulated abnormally high anthocyanins.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Antisense Inhibition of RNS1 orRNS2t0
- Gene
- RNS2t0
- Outcome
- levels of anthocyaninse0
- Direction
- abnormally highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants contained abnormally high levels of anthocyanins”
t0“Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation1”
Claim · model-written summary
The authors infer distinct functions for Arabidopsis RNS1 and RNS2.
Verbatim
e0“These results, together with the differential localization of the proteins, imply that RNS1 and RNS2 have distinct functions in the plant”
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