Abstract
Root colonization of Arabidopsis thaliana by the nonpathogenic, rhizosphere-colonizing, biocontrol bacterium Pseudomonas fluorescens WCS417r has been shown to elicit induced systemic resistance (ISR) against Pseudomonas syringae pv. tomato (Pst). The ISR response differs from the pathogen-inducible systemic acquired resistance (SAR) response in that ISR is independent of salicylic acid and not associated with pathogenesis-related proteins. Several ethylene-response mutants were tested and showed essentially normal symptoms of Pst infection. ISR was abolished in the ethylene-insensitive mutant etr1-1, whereas SAR was unaffected. Similar results were obtained with the ethylene-insensitive mutants ein2 through ein7, indicating that the expression of ISR requires the complete signal-transduction pathway of ethylene known so far. The induction of ISR by WCS417r was not accompanied by increased ethylene production in roots or leaves, nor by increases in the expression of the genes encoding the ethylene biosynthetic enzymes 1-aminocyclopropane-1-carboxylic (ACC) synthase and ACC oxidase. The eir1 mutant, displaying ethylene insensitivity in the roots only, did not express ISR upon application of WCS417r to the roots, but did exhibit ISR when the inducing bacteria were infiltrated into the leaves. These results demonstrate that, for the induction of ISR, ethylene responsiveness is required at the site of application of inducing rhizobacteria.
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Claims 8
Claim · model-written summary
Ethylene response mutants had essentially normal symptoms after Pst infection.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- ethylene-response mutantse0
- Interactor
- Pste0pathogen
- Outcome
- normal symptoms of Pst infectione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several ethylene-response mutants were tested and showed essentially normal symptoms of Pst infection.”
t0“Arabidopsis”
Claim · model-written summary
ISR was abolished in etr1-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- etr1-1e0
- Interactor
- Pseudomonas fluorescens WCS417r
.JPG?width=60)
Pseudomonas fluorescense1biocontrol_agent - Outcome
- ISRe0
- Direction
- abolishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“ISR was abolished in the ethylene-insensitive mutant etr1-1, whereas SAR was unaffected.”
t0“Arabidopsis”
e1“Pseudomonas fluorescens WCS417r”
Claim · model-written summary
SAR was unaffected in etr1-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- etr1-1e0
- Outcome
- SARe0
- Direction
- unaffectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ISR was abolished in the ethylene-insensitive mutant etr1-1, whereas SAR was unaffected.”
t0“Arabidopsis”
Claim · model-written summary
The ein2 through ein7 mutants gave similar ISR results.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ein2 through ein7e0
- Outcome
- ISRe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar results were obtained with the ethylene-insensitive mutants ein2 through ein7, indicating that the expression of ISR requires the complete signal-transduction pathway of ethylene known so far.”
t0“Arabidopsis”
Claim · model-written summary
WCS417r induction of ISR did not increase ethylene production in roots or leaves.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- WCS417re0
- Interactor
- WCS417re0biocontrol_agent
- Outcome
- ethylene production in roots or leavese0
- Direction
- not accompanied by increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of ISR by WCS417r was not accompanied by increased ethylene production in roots or leaves, nor by increases in the expression of the genes encoding the ethylene biosynthetic enzymes 1-aminocyclopropane-1-carboxylic (ACC) synthase and ACC oxidase.”
t0“Arabidopsis”
Claim · model-written summary
WCS417r induction did not increase expression of ACC synthase and ACC oxidase genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- WCS417re0
- Interactor
- WCS417re0biocontrol_agent
- Gene
- ACC oxidasee0
- Outcome
- expression of the genes encoding the ethylene biosynthetic enzymese0
- Direction
- nor by increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of ISR by WCS417r was not accompanied by increased ethylene production in roots or leaves, nor by increases in the expression of the genes encoding the ethylene biosynthetic enzymes 1-aminocyclopropane-1-carboxylic (ACC) synthase and ACC oxidase.”
t0“Arabidopsis”
Claim · model-written summary
Root application of WCS417r did not induce ISR in eir1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eir1e0
- Treatment
- application of WCS417r to the rootse0
- Interactor
- WCS417re0biocontrol_agent
- Outcome
- ISRe0
- Direction
- did not expresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The eir1 mutant, displaying ethylene insensitivity in the roots only, did not express ISR upon application of WCS417r to the roots, but did exhibit ISR when the inducing bacteria were infiltrated into the leaves.”
t0“Arabidopsis”
Claim · model-written summary
Leaf infiltration of WCS417r induced ISR in eir1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- eir1e0
- Treatment
- inducing bacteria were infiltrated into the leavese0
- Interactor
- WCS417re0biocontrol_agent
- Outcome
- ISRe0
- Direction
- did exhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The eir1 mutant, displaying ethylene insensitivity in the roots only, did not express ISR upon application of WCS417r to the roots, but did exhibit ISR when the inducing bacteria were infiltrated into the leaves.”
t0“Arabidopsis”
The same questions in other papers
inoculation · disease · ArabidopsisPositive and negative results
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- negative
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.