Abstract
Sense and antisense strategies for interfering with the replication of brome mosaic virus (BMV) were examined. The effects of 200 nucleotide-long sense and antisense transcripts, corresponding to the viral 3' end (-) strand promoter, on the accumulation of progeny viral RNAs were studied by co-inoculation with wildtype BMV RNAs. Progeny accumulation in barley protoplasts transfected with either sense or antisense transcripts of the (-) strand promoter and BMV RNAs-1 and -2 was decreased by more than 90%, and by 60 to 80% when RNA-3 was also present. This trans interference was concentration-dependent, and reduced both (+) and (-) strand progeny accumulation to a similar extent. The appearance of complementary (-) strands indicated that sense interfering transcripts could serve as templates for (-) strand synthesis, and the use of deletion mutants revealed that the observed interference was in part mediated by this template activity. The reproducibility of the protoplast assay used here allows rapid evaluation of interference strategies and comparisons to be made of alternative approaches to engineered resistance. The results presented here suggest that targeting viral (-) strand promoters with sense and antisense transcripts may be an effective method for engineering plant resistance to viral infection.
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Claims 8
Claim · model-written summary
Sense or antisense promoter transcripts reduced viral progeny RNA accumulation by more than 90% with RNAs 1 and 2.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- either sense or antisense transcripts of the (-) strand promotere0
- Interactor
- BMV
Brome mosaic viruse0pathogen - Outcome
- Progeny accumulatione0
- Direction
- decreasede0
- Value
- more than 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Progeny accumulation in barley protoplasts transfected with either sense or antisense transcripts of the (-) strand promoter and BMV RNAs-1 and -2 was decreased by more than 90%”
Claim · model-written summary
With RNA 3 present, promoter transcripts reduced viral progeny accumulation by 60 to 80%.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- either sense or antisense transcripts of the (-) strand promotere0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- Progeny accumulatione0
- Direction
- decreasede0
- Value
- 60 to 80%e0
- Qualifier
- when RNA-3 was also presente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Progeny accumulation in barley protoplasts transfected with either sense or antisense transcripts of the (-) strand promoter and BMV RNAs-1 and -2 was decreased by more than 90%, and by 60 to 80% when RNA-3 was also present”
Claim · model-written summary
Trans interference depended on transcript concentration.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- concentration-dependente0
- Outcome
- trans interferencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This trans interference was concentration-dependent”
e1“barley protoplasts”
Claim · model-written summary
Trans interference reduced positive and negative strand viral progeny similarly.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- both (+) and (-) strand progeny accumulatione0
- Direction
- reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced both (+) and (-) strand progeny accumulation to a similar extent”
e1“barley protoplasts”
Claim · model-written summary
Sense interfering transcripts served as templates for minus-strand synthesis.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- templates for (-) strand synthesise0
- Qualifier
- sense interfering transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The appearance of complementary (-) strands indicated that sense interfering transcripts could serve as templates for (-) strand synthesis”
e1“barley protoplasts”
Claim · model-written summary
Deletion mutants indicated template activity partly mediated interference.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- observed interferencee0
- Qualifier
- template activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the use of deletion mutants revealed that the observed interference was in part mediated by this template activity”
e1“barley protoplasts”
Claim · model-written summary
The protoplast assay was reproducible enough for rapid comparison of resistance strategies.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- rapid evaluation of interference strategiese0
- Comparator
- alternative approaches to engineered resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The reproducibility of the protoplast assay used here allows rapid evaluation of interference strategies and comparisons to be made of alternative approaches to engineered resistance”
e1“barley protoplasts”
Claim · model-written summary
The authors suggested targeting viral minus-strand promoters for engineered plant virus resistance.
- Taxon
- barleyHordeum vulgaree1
- Outcome
- engineering plant resistance to viral infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“targeting viral (-) strand promoters with sense and antisense transcripts may be an effective method for engineering plant resistance to viral infection”
e1“barley protoplasts”
The same questions in other papers
loss of function · Brome mosaic virus · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.