Claim · model-written summary
With RNA 3 present, promoter transcripts reduced viral progeny accumulation by 60 to 80%.
- Taxon
- barley
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Hordeum vulgaree0 - Treatment
- either sense or antisense transcripts of the (-) strand promotere0
- Interactor
- BMV
Brome mosaic viruse0pathogen - Outcome
- Progeny accumulatione0
- Direction
- decreasede0
- Value
- 60 to 80%e0
- Qualifier
- when RNA-3 was also presente0
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Progeny accumulation in barley protoplasts transfected with either sense or antisense transcripts of the (-) strand promoter and BMV RNAs-1 and -2 was decreased by more than 90%, and by 60 to 80% when RNA-3 was also present”
Title
Interference with brome mosaic virus replication by targeting the minus strand promoter
C. C. Huntley; T. C. Hall Journal of General Virology 1993. doi:10.1099/0022-1317-74-11-2445
Other claims from this paper (7)
- Intervention effect Sense or antisense promoter transcripts reduced viral progeny RNA accumulation by more than 90% with RNAs 1 and 2.
- Association Trans interference depended on transcript concentration.
- Intervention effect Trans interference reduced positive and negative strand viral progeny similarly.
- Mechanism Sense interfering transcripts served as templates for minus-strand synthesis.
- Mechanism Deletion mutants indicated template activity partly mediated interference.
- Method / resource The protoplast assay was reproducible enough for rapid comparison of resistance strategies.
- Recommendation The authors suggested targeting viral minus-strand promoters for engineered plant virus resistance.