Abstract
The response of developing maize (Zea mays L.) endosperm to elevated levels of abscisic acid (ABA) was investigated. Maize kernels and subtending cob sections were excised at 5 days after pollination (DAP) and placed in culture with or without 90 micromolar (+/-)-ABA in the medium. A decreased number of cells per endosperm was observed at 10 DAP (and later sampling times) in kernels cultured in medium containing ABA from 5 DAP, and in kernels transferred at 8 DAP to medium containing ABA, but not in kernels transferred at 11 DAP to medium containing ABA. The number of starch granules per endosperm was decreased in some treatments, but the reduction, when apparent, was comparable to the decreased number of endosperm cells. The effect on endosperm fresh weight was slight, transient, and appeared to be secondary to the effect on cell number. Mature endosperm dry weight was reduced when kernels were cultured continuously in medium containing ABA. Endosperm (+)-ABA content of kernels cultured in 0, 3, 10, 30, 100, or 300 micromolar (+/-)-ABA was measured at 10 DAP by indirect ELISA using a monoclonal antibody. Content of (+)-ABA in endosperms correlated negatively (R = -0.92) with endosperm cell number. On the basis of these studies we propose that during early kernel development, elevated levels of ABA decrease the rate of cell division in maize endosperm which, in turn, could limit the storage capacity of the kernel.
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Claims 9
Claim · model-written summary
ABA from 5 DAP reduced endosperm cell number at 10 DAP and later.
- Organ / stage
- endosperme0
- Treatment
- medium containing ABA from 5 DAPe0
- Outcome
- number of cells per endosperme0
- Direction
- decreasede0
- Qualifier
- at 10 DAP (and later sampling times)e0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decreased number of cells per endosperm was observed at 10 DAP (and later sampling times) in kernels cultured in medium containing ABA from 5 DAP”
t0“Cultured Maize Kernels”
Claim · model-written summary
Transfer to ABA at 8 DAP reduced endosperm cell number.
- Treatment
- transferred at 8 DAP to medium containing ABAe0
- Outcome
- number of cells per endosperme0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decreased number of cells per endosperm was observed at 10 DAP (and later sampling times) in kernels cultured in medium containing ABA from 5 DAP, and in kernels transferred at 8 DAP to medium containing ABA”
t0“Cultured Maize Kernels”
Claim · model-written summary
Transfer to ABA at 11 DAP did not reduce endosperm cell number.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in kernels transferred at 11 DAP to medium containing ABA”
t0“Cultured Maize Kernels”
t1“number of cells per endosperm”
Claim · model-written summary
Some ABA treatments reduced starch granule count in proportion to endosperm cell count.
- Treatment
- some treatmentse0
- Outcome
- number of starch granules per endosperme0
- Direction
- decreasede0
- Qualifier
- comparable to the decreased number of endosperm cellse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of starch granules per endosperm was decreased in some treatments, but the reduction, when apparent, was comparable to the decreased number of endosperm cells”
t0“Cultured Maize Kernels”
Claim · model-written summary
ABA had a slight, transient effect on endosperm fresh weight.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect on endosperm fresh weight was slight, transient, and appeared to be secondary to the effect on cell number”
t0“Cultured Maize Kernels”
t1“abscisic acid (ABA) was investigated”
Claim · model-written summary
Continuous ABA culture reduced mature endosperm dry weight.
- Treatment
- cultured continuously in medium containing ABAe0
- Outcome
- Mature endosperm dry weighte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature endosperm dry weight was reduced when kernels were cultured continuously in medium containing ABA”
t0“Cultured Maize Kernels”
Claim · model-written summary
Endosperm ABA content was negatively correlated with cell number, R = -0.92.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Content of (+)-ABA in endosperms correlated negatively (R = -0.92) with endosperm cell number”
t0“Cultured Maize Kernels”
Claim · model-written summary
The authors propose elevated ABA reduces cell division early in kernel development.
- Compound
- ABAe0
- Outcome
- rate of cell divisione0
- Direction
- decreasee0
- Qualifier
- during early kernel developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“during early kernel development, elevated levels of ABA decrease the rate of cell division in maize endosperm”
Claim · model-written summary
The authors propose reduced cell division could limit kernel storage capacity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which, in turn, could limit the storage capacity of the kernel”
t0“Cultured Maize Kernels”
t1“cell division”
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(+)-abscisic acid · growth · Zea mays (maize)Results differ
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See all 87 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.