Abstract
Using Arabidopsis, we analyzed the effect of omission of a nitrogen source and of the addition of different nitrogen-containing compounds on the extractable activity and the enzyme and mRNA accumulation of adenosine 5'-phosphosulfate reductase (APR). During 72 h without a nitrogen source, the APR activity decreased to 70% and 50% of controls in leaves and roots, respectively, while cysteine (Cys) and glutathione contents were not affected. Northern and western analysis revealed that the decrease of APR activity was correlated with decreased mRNA and enzyme levels. The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS). (35)SO(4)(2-) feeding showed that after addition of NH(4)(+), Gln, or OAS to nitrogen-starved plants, incorporation of (35)S into proteins significantly increased in roots; however, glutathione and Cys labeling was higher only with Gln and OAS or with OAS alone, respectively. OAS strongly increased mRNA levels of all three APR isoforms in roots and also those of sulfite reductase, Cys synthase, and serine acetyltransferase. Our data demonstrate that sulfate reduction is regulated by nitrogen nutrition at the transcriptional level and that OAS plays a major role in this regulation.
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Claims 15
Claim · model-written summary
Without a nitrogen source for 72 h, APR activity in Arabidopsis leaves decreased to 70% of controls.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- leavese0
- Treatment
- without a nitrogen sourcee0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- decreasede0
- Value
- 70%e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“During 72 h without a nitrogen source, the APR activity decreased to 70% and 50% of controls in leaves and roots, respectively”
Claim · model-written summary
Without a nitrogen source for 72 h, APR activity in Arabidopsis roots decreased to 50% of controls.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- without a nitrogen sourcee0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- decreasede0
- Value
- 50%e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“During 72 h without a nitrogen source, the APR activity decreased to 70% and 50% of controls in leaves and roots, respectively”
Claim · model-written summary
Cysteine and glutathione contents were not affected by 72 h without a nitrogen source.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- without a nitrogen sourcee0
- Outcome
- cysteine (Cys) and glutathione contentse1
- Direction
- not affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“During 72 h without a nitrogen source”
e1“while cysteine (Cys) and glutathione contents were not affected”
Claim · model-written summary
The decrease of APR activity was correlated with decreased mRNA and enzyme levels.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- APRe0
- Outcome
- decrease of APR activitye0
- Direction
- decreasede0
- Qualifier
- decreased mRNA and enzyme levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“the decrease of APR activity was correlated with decreased mRNA and enzyme levels”
Claim · model-written summary
The reduced APR activity in N-starved roots was fully restored within 24 h by 4 mM nitrate.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- addition of 4 mM each of NO(3)(-)e0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- restorede0
- Value
- 4 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS)”
Claim · model-written summary
The reduced APR activity in N-starved roots was fully restored within 24 h by 4 mM ammonium.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- NH(4)(+)e0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- restorede0
- Value
- 4 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS)”
Claim · model-written summary
The reduced APR activity in N-starved roots was fully restored within 24 h by 4 mM glutamine.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- glutamine (Gln)e0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- restorede0
- Value
- 4 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS)”
Claim · model-written summary
The reduced APR activity in N-starved roots was fully restored within 24 h by 1 mM O-acetylserine.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- 1 mM O-acetylserine (OAS)e0
- Gene
- APRe0
- Outcome
- APR activitye0
- Direction
- restorede0
- Value
- 1 mMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS)”
Claim · model-written summary
After NH4+, Gln or OAS was added to nitrogen-starved plants, incorporation of 35S into root proteins significantly increased.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- addition of NH(4)(+), Gln, or OASe0
- Outcome
- incorporation of (35)S into proteinse0
- Direction
- increasede0
- Comparator
- nitrogen-starved plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“after addition of NH(4)(+), Gln, or OAS to nitrogen-starved plants, incorporation of (35)S into proteins significantly increased in roots”
Claim · model-written summary
Glutathione labeling was higher only with Gln and OAS after addition to nitrogen-starved plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Gln and OASe0
- Outcome
- glutathionee0
- Direction
- highere0
- Qualifier
- only with Gln and OASe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“glutathione and Cys labeling was higher only with Gln and OAS or with OAS alone, respectively”
Claim · model-written summary
Cysteine labeling was higher only with OAS after addition to nitrogen-starved plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- OAS alonee0
- Outcome
- Cys labelinge0
- Direction
- highere0
- Qualifier
- onlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“glutathione and Cys labeling was higher only with Gln and OAS or with OAS alone, respectively”
Claim · model-written summary
OAS strongly increased mRNA levels of all three APR isoforms in roots.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Treatment
- OASe0
- Gene
- all three APR isoformse0
- Outcome
- mRNA levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“OAS strongly increased mRNA levels of all three APR isoforms in roots and also those of sulfite reductase, Cys synthase, and serine acetyltransferase”
Claim · model-written summary
OAS also increased mRNA levels of sulfite reductase, Cys synthase and serine acetyltransferase.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- OASe0
- Gene
- sulfite reductase, Cys synthase, and serine acetyltransferasee0
- Outcome
- mRNA levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“OAS strongly increased mRNA levels of all three APR isoforms in roots and also those of sulfite reductase, Cys synthase, and serine acetyltransferase”
Claim · model-written summary
Sulfate reduction is regulated by nitrogen nutrition at the transcriptional level.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- nitrogen nutritione0
- Outcome
- sulfate reductione0
- Qualifier
- transcriptional levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“sulfate reduction is regulated by nitrogen nutrition at the transcriptional level”
Claim · model-written summary
OAS plays a major role in the regulation of sulfate reduction by nitrogen.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- OASe0
- Outcome
- major role in this regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“OAS plays a major role in this regulation”
The same questions in other papers
glutamine · molecular: metabolites · ArabidopsisResults agree
1 paper in the same direction as this paper
- positive
nitrogen · molecular: metabolites · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 9 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.