Abstract
To establish an efficient propagation protocol, plants were regenerated from mature leaf and petiole explants through direct organogenesis without intervening callus phase. The results show that leaf was the best explant for shoot regeneration when cultured on Murashige and Skoog (MS) medium supplemented with 0.2 mgl-1 α-naphthalene acetic acid (NAA), 0.3 mgl-1 zeatin and 0.3 mgl-1 6-benzylaminopurine(BAP). Rooting was enhanced on rooting medium containing activated charcoal (AC) supplemented with 0.25 mgl-1 indol-3-butyric acid (IBA). This protocol provides a basis for future studies on genetic improvement of Silybum Marianum and it can be basis for future studies on genetic improvement. Key words: Direct regeneration, activated charcoal, leaf, petiole, Silybum marianum.
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Claims 2
Claim · model-written summary
Leaf explants gave the best shoot regeneration of Silybum marianum by direct organogenesis, compared with petiole explants.
- Taxon
- Silybum marianum
Silybum marianumt0 - Organ / stage
- mature leafe1
- Treatment
- explant typet0
- Outcome
- shoot regeneratione0
- Direction
- beste0
- Comparator
- petiolee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of explant type, plant growth regulators and activated charcoal on direct organogenesis of Silybum marianum”
e0“The results show that leaf was the best explant for shoot regeneration when cultured on Murashige and Skoog (MS) medium supplemented with 0.2 mgl-1 α-naphthalene acetic acid (NAA), 0.3 mgl-1 zeatin and 0.3 mgl-1 6-benzylaminopurine(BAP)”
e1“plants were regenerated from mature leaf and petiole explants”
Claim · model-written summary
Rooting of Silybum marianum was enhanced on medium with activated charcoal and IBA.
- Taxon
- Silybum marianumSilybum marianumt0
- Treatment
- rooting medium containing activated charcoal (AC) supplemented with 0.25 mgl-1 indol-3-butyric acid (IBA)e2
- Outcome
- Rootinge2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Effects of explant type, plant growth regulators and activated charcoal on direct organogenesis of Silybum marianum”
e2“Rooting was enhanced on rooting medium containing activated charcoal (AC) supplemented with 0.25 mgl-1 indol-3-butyric acid (IBA)”
The same questions in other papers
in vitro · growth · Silybum marianum (milk thistle)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.